A control method for a digital nucleic acid amplification instrument

Abstract

The invention provides a control method for a digital nucleic acid amplification instrument, which includes: step 1, injecting nucleic acid into the first reaction chamber; step 2, operating a heating device, so that the nucleic acid in the first reaction chamber is thermally split , the first control valve is opened, so that the split nucleic acid enters the second reaction chamber through the diversion pipeline; Step 3, the output control valve is opened, so that the intermediate in the output storage bin flows into the second reaction chamber ; Step 4, the controller controls the cooling device to start working to cool down the second reaction chamber; Step 5, the heating device in the second reaction chamber starts to work, and the controller controls the second control valve to open , the controller also controls the pump body to work at the same time, so as to transport the nucleic acid in the second reaction chamber back to the first reaction chamber; step 6, setting the step 1 to the step 5 as a cycle Reaction, repeat this cycle reaction 20-30 times.

Description

technical field [0001] The present invention generally relates to a nucleic acid amplification instrument, and more particularly relates to a control method of a digital nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification is a general term for a large class of technical methods, currently including conventional PCR, real-time fluorescent PCR, isothermal nucleic acid amplification technology, etc. Nucleic acid amplification is very useful in molecular biology and is widely used in biology, therapeutics, diagnostics, forensic And every aspect of the study has broad applicability. Typically, one or more primers are used to generate an amplicon from a starting template, wherein the amplicon corresponds to or is complementary to the template from which the amplicon was generated. Multiplex amplification also simplifies the process and reduces costs. [0003] The existing nucleic acid amplification technology mainly includes: heating t...

AI Technical Summary

Problems solved by technology

[0004] The existing nucleic acid amplification instrument is not highly intelligent. In the process of nucleic acid amplification, the addition of primers, buffers, and dNTPs and other components requires manual addition by staff; at the same time, in the process of nucleic acid amplification, often need To amplify nucleic acid multiple times, there are requirements for temperature control in each nucleic acid amplification process, and these operations need to be performed manually, which is time-consuming and laborious, which is not conducive to production and research; therefore, the present invention provides a digital nucleic acid A regulation method of an amplification instrument, to at least partially solve the above-mentioned problems

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