Optical excitation and detection method of nucleic acid amplifier

Abstract

The present invention provides an optical excitation and detection method of a nucleic acid amplifier. The optical excitation and detection method comprises the following steps: nucleic acid is injected into a first reaction cavity; a heating device works to enable the nucleic acid in the first reaction cavity to be heated to split, a first control valve is opened, so that the split nucleic acid enters a second reaction cavity through a flow guide pipeline; an output material control valve is opened to enable an intermediate material in an output material storage bin to flow into the second reaction cavity; a controller controls a heat dissipation device to start to work to cool the second reaction cavity; a heating device in the second reaction cavity starts to work, the controller controls a second control valve to be opened, meanwhile, the controller further controls a pump body to work, so that the nucleic acid in the second reaction cavity is conveyed back into the first reactioncavity; the controller analyzes data acquired by a fluorescence detection unit and calculates a relationship between a system stability value delta s and a measurement interval K to select a corresponding working mode; and the reaction is repeated for 20-30 times.

Description

technical field [0001] The present invention generally relates to a nucleic acid amplification instrument, and more particularly relates to an optical excitation and detection method for a nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification is a general term for a large class of technical methods, currently including conventional PCR, real-time fluorescent PCR, isothermal nucleic acid amplification technology, etc. Nucleic acid amplification is very useful in molecular biology and is widely used in biology, therapeutics, diagnostics, forensic And every aspect of the study has broad applicability. Typically, one or more primers are used to generate an amplicon from a starting template, wherein the amplicon corresponds to or is complementary to the template from which the amplicon was generated. Multiplex amplification also simplifies the process and reduces costs. [0003] The existing nucleic acid amplification technology mainly ...

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Problems solved by technology

[0004] The existing nucleic acid amplification instrument is not highly intelligent. In the process of nucleic acid amplification, the addition of primers, buffers, dNTP and other components requires manual addition by staff; at the same time, in the process of nucleic acid amplification, it is often necessary To amplify nucleic acid multiple times, temperature control is required in each nucleic acid amplification process, and these operations need to be performed manually, which is time-consuming and laborious, and is not conducive to production and research; therefore, the present invention provides a nucleic acid amplification method. Intensified optical excitation and detection methods to at least partially address the above-mentioned problems

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