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Preparation and application of a polycyclic aromatic hydrocarbon (pahs) degrading bacterial agent

A technology for polycyclic aromatic hydrocarbons and degrading bacteria, which can be used in the restoration of polluted soil, fixing on or in inorganic carriers, separating microorganisms, etc. and density, enhancing soil fertility

Active Publication Date: 2022-03-22
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The PAH-degrading bacteria agents in the market are often composed of free bacteria artificially domesticated in the laboratory, which are not suitable for practical engineering applications in the environment

Method used

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  • Preparation and application of a polycyclic aromatic hydrocarbon (pahs) degrading bacterial agent
  • Preparation and application of a polycyclic aromatic hydrocarbon (pahs) degrading bacterial agent
  • Preparation and application of a polycyclic aromatic hydrocarbon (pahs) degrading bacterial agent

Examples

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Effect test

Embodiment 1

[0026] Embodiment 1, a kind of preparation method of polycyclic aromatic hydrocarbons (PAHs) degrading bacterial agent, comprises the following steps:

[0027] In the first step, the iron-rich sludge was roasted at 500°C for 5 hours, cooled to room temperature, ground into powder, and passed through a 300-mesh sieve to obtain iron-rich sludge biochar, such as figure 1 shown, from figure 1 It can be seen that a large number of pore structures are formed on the surface of the iron-rich sludge biochar at this time;

[0028] Step 2: Weigh 5g of soil sample into 100mL of oil-reducing medium, culture at 140rpm, shake at 25°C for 7 days to obtain culture medium; pipette 1mL of culture solution and add it to 100mL of oil-reducing medium to continue acclimatization for 1d; repeat acclimation culture Step, continuous enrichment culture 10 times, realize the enrichment of petroleum degrading bacteria, obtain the enrichment solution;

[0029] The third step is to take the enrichment sol...

Embodiment 2

[0033] Embodiment 2, a kind of preparation method of polycyclic aromatic hydrocarbons (PAHs) degrading bacterial agent, comprises the following steps:

[0034] In the first step, the iron-rich sludge is roasted at 300°C for 5 hours, cooled to room temperature, ground into powder, and passed through a 300-mesh sieve to obtain iron-rich sludge biochar;

[0035] The second step is to weigh 200g of soil samples into 5000mL of oil-reducing medium, 140rpm, 25°C shaker culture for 7d to obtain the culture solution; pipette 20mL of culture solution and add it to 2000mL of oil-reducing medium to continue acclimatization for 4 days; repeat the acclimatization culture step, continuously enriching and culturing 5 times to realize the enrichment of petroleum-degrading bacteria and obtain the enrichment solution;

[0036] The third step is to take the enrichment solution and use the dilution factor method to serially dilute 10 -4 ~10 -7 Then spread it on the solid oil-reduced medium with ...

Embodiment 3

[0040] Embodiment 3, a kind of preparation method of polycyclic aromatic hydrocarbons (PAHs) degrading bacterial agent, comprises the following steps:

[0041] In the first step, iron-rich sludge is roasted at 700°C for 1 hour, cooled to room temperature, ground into powder, and passed through a 300-mesh sieve to obtain iron-rich sludge biochar;

[0042] Step 2: Weigh 1g of soil sample into 500mL oil-reduced medium, culture at 140rpm, shake on a shaker at 25°C for 2 days to obtain a culture solution; pipette 5mL culture solution and add it to 500mL oil-reduced medium to continue acclimatization for 7 days; repeat acclimatization culture step, continuously enriching and culturing 5 times to realize the enrichment of petroleum-degrading bacteria and obtain the enrichment solution;

[0043] The third step is to take the enrichment solution and use the dilution factor method to serially dilute 10 -4 ~10 -7 Then spread it on the solid oil-reduced medium with polycyclic aromatic h...

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Abstract

Preparation and application of a polycyclic aromatic hydrocarbon (PAHs) degrading bacterial agent. The preparation is to roast the iron-rich sludge, cool and grind to obtain the iron-rich sludge biochar; cultivate the soil in the oil-reducing medium to obtain the culture solution, and pipette The culture solution was added to the oil-reducing medium for acclimatization, and the enrichment solution was repeatedly obtained; the enrichment solution was diluted and spread on the solid oil-reducing medium with polycyclic aromatic hydrocarbons as the carbon source, and placed in a biochemical incubator for continuous cultivation until there was Colony generation: Pick a single colony and purify it to obtain a single strain for PAH degradation, mix the suspension of the single strain in equal volumes to obtain DT; finally mix iron-rich sludge biochar with DT, cultivate, filter and dry to obtain PAH-degrading bacteria agent; the application is to uniformly mix polluted soil, water and polycyclic aromatic hydrocarbon degrading bacteria agent to obtain a mixture, and maintain to remove polycyclic aromatic hydrocarbons in polluted soil; the present invention accelerates the degradation of polycyclic aromatic hydrocarbons, has no secondary pollution carrier, and biochar The addition of can improve soil physical and chemical properties and enhance soil fertility.

Description

technical field [0001] The invention belongs to the technical field of soil polycyclic aromatic hydrocarbons (PAHs) pollution control, in particular to the preparation and application of a polycyclic aromatic hydrocarbons (PAHs) degrading bacterial agent. Background technique [0002] In recent years, with the great increase of oil production, the process of oil extraction, transportation, processing and sales will cause oil leakage. As one of the main components of petroleum, polycyclic aromatic hydrocarbons have three effects of carcinogenicity, teratogenicity and mutagenicity. Because they cannot be biodegraded, they persist in the soil for a long time, causing soil pollution and endangering public health and the surrounding environment. [0003] The remediation of PAH-contaminated soil mainly includes physical remediation, biological remediation and chemical remediation. The cost of physical remediation is high, and the amount of work for high-concentration PAH-contamina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N1/02B09C1/10
CPCC12N11/14C12N1/02B09C1/10
Inventor 梁继东滕庭庭武子俊
Owner XI AN JIAOTONG UNIV
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