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Breast cancer 21 genetic test reagent kit and application method thereof

A gene detection and kit technology, applied in the fields of biotechnology and medicine, can solve the problems of easy errors, time-consuming, cumbersome operation process and other problems in the gene matching reaction well

Inactive Publication Date: 2020-06-02
宁波美丽人生医药生物科技发展有限公司
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Problems solved by technology

[0005] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a breast cancer 21 gene detection kit and a method of use thereof, the purpose of which is to solve the problem that errors are easily generated during the detection experiment operation of gene-combined reaction wells in the prior art, and the operation process cumbersome and time-consuming technical issues

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  • Breast cancer 21 genetic test reagent kit and application method thereof
  • Breast cancer 21 genetic test reagent kit and application method thereof
  • Breast cancer 21 genetic test reagent kit and application method thereof

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[0125] In order to make the purpose, technical solutions and advantages of the present invention clearer, the following in conjunction with specific examples, and with reference to the appended figure 1 , to further describe the present invention in detail.

[0126] Breast cancer 21 gene detection kit, including 21 detection genes, the 21 detection genes are as follows: Ki67, STK15, Survivin, CCNB1, MYBL2, MMP11, CTSL2, GRB7, HER2, ER, PGR, BCL2, SCUBE2, GSTM1, BAG1, CD68, β-actin, GAPDH, RPLPO, GUS, and TFRC;

[0127] One pair of primers and one specific probe were designed for each of the 21 detection genes, and the primer sequences and Taqman probe sequences corresponding to the 21 detection genes were as follows:

[0128] (1) The primer sequences for detecting the Ki67 gene are: SEQ ID NO: 1 and SEQ ID NO: 2, and the Taqman probe sequence for detecting the Ki67 gene is: SEQ ID NO: 3; details are as follows:

[0129] Ki67:

[0130] F) P1-KI67 5'-CTCCATGTGCCTGAGAT-3' (SEQ...

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Abstract

The invention belongs to the fields of biological technology and medicines, and particularly relates to a breast cancer 21 genetic test reagent kit. The breast cancer 21 genetic test reagent kit comprises a specific primer for detecting Ki67,STK15, Survivin, CCNB1, MYBL2, MMP11, CTSL2, GRB7, HER2, ER, PGR, BCL2, SCUBE2, GSTM1, BAG1, CD68, beta-actin, GAPDH, RPLP0, GUS and TFRC genes, a probe, a positive quality control product, common detection assemblies for fluorescent quantitation PCR detection. The invention further provides an application method of the reagent kit. The reagent kit is usedfor assessing the recurring risk and chemotherapy benefit degrees of patients suffering from breast cancer by detecting 21 genes associated with human breast cancer, and the reagent kit has the characteristics of being high in sensitivity, high in specificity, simple in detection operation and the like.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and in particular relates to a breast cancer 21 gene detection kit and an application method thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. In recent years, surgery has been the mainstay, and radiotherapy, chemotherapy, and endocrine therapy have been used as adjuvant multidisciplinary comprehensive treatment models to significantly reduce the recurrence and mortality of breast cancer. With the development of genomics, scholars have found that the detection of some polygenic markers may be more accurate in predicting tumor recurrence than clinical case indicators. In order to judge whether patients benefit from chemotherapy and avoid overtreatment. [0003] The 21-gene test first proposed by Paik et al. is a clinically proven method for assessing the risk of recurrence in patients with ER-positive, axillary lymph node-negative ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/118C12Q2600/166C12Q2531/113C12Q2561/101C12Q2563/107C12Q2545/101
Inventor 田晓丽
Owner 宁波美丽人生医药生物科技发展有限公司
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