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A macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and its preparation method

A technology for tissue engineering scaffolds and active ingredients, which can be used in pharmaceutical formulations, filament/thread forming, textiles, and papermaking, etc. It can solve the problems of lack of large pores for cell penetration and tissue regeneration, high brittleness of phosphorous electrospun fibers, and high energy consumption. problems, to achieve the effects of infiltration growth and osteogenic differentiation, improvement of early biological activity deficiency, increase of pore size and surface roughness

Active Publication Date: 2021-12-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of this technology is cumbersome, consumes a lot of energy, and the prepared phosphorus electrospun fibers are brittle, and the mechanical properties do not meet the requirements. At the same time, there is also a potential risk of "blood calcium"
In addition, the scaffolds prepared by the three methods mentioned above will only have submicron-scale apparent small pores due to the accumulation of traditional electrospun nano or micro fibers, and lack the macropores necessary for cell penetration and tissue regeneration.

Method used

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  • A macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and its preparation method
  • A macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and its preparation method
  • A macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and its preparation method

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Embodiment 1

[0030] This embodiment provides a macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active components, which is characterized in that it consists of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning, and the nano-scale fibers The inorganic active substances are released preferentially, and the inorganic active substances in the beads are subsequently released after the organic matrix of the beads is gradually degraded.

[0031] The preparation method of the macroporous bone tissue engineering scaffold capable of sustainably releasing the inorganic active ingredient:

[0032]Step 1: Dissolve 0.8g of PCL (number-average molecular weight: 80,000) in 8mL of dichloromethane (purity≥99.5%), and magnetically stir for 6h to obtain a clear and transparent polymer solution.

[0033] Step 2: Add 0.08 g of tricalcium phosphate into 2 mL of dichloromethane (purity ≥ 99.5%), and ultrasonically disp...

Embodiment 2

[0038] This embodiment provides a macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active components, which is characterized in that it consists of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning, and the nano-scale fibers The inorganic active substances are released preferentially, and the inorganic active substances in the beads are subsequently released after the organic matrix of the beads is gradually degraded.

[0039] The preparation method of the macroporous bone tissue engineering scaffold capable of sustainably releasing the inorganic active ingredient:

[0040] Step 1: Dissolve 0.5g chitosan (number average molecular weight 50,000-300,000, deacetylation degree: 90%) in 5mL 98wt% acetic acid solution, and magnetically stir for 12h to obtain a clear and transparent polymer solution.

[0041] Step 2: Add 0.04 g of silicon dioxide into 5 mL of 98 wt% acetic acid solution, an...

Embodiment 3

[0046] This embodiment provides a macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active components, which is characterized in that it consists of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning, and the nano-scale fibers The inorganic active substances are released preferentially, and the inorganic active substances in the beads are subsequently released after the organic matrix of the beads is gradually degraded.

[0047] The preparation method of the macroporous bone tissue engineering scaffold capable of sustainably releasing the inorganic active ingredient:

[0048] Step 1: Dissolve 0.6g PLLA (number-average molecular weight: 20,000) in 8mL chloroform, and stir magnetically for 12 hours to obtain a clear and transparent polymer solution.

[0049] Step 2: Add 0.1 g of hydroxyapatite into 2 mL of chloroform, and disperse by ultrasonic wave for 6 hours, so that the tricalcium ph...

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Abstract

The invention discloses a macroporous bone tissue engineering support capable of continuously releasing inorganic active ingredients and a preparation method thereof. The macroporous bone tissue engineering scaffold is composed of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning. The inorganic active substances in the nano-scale fibers are released preferentially. After the organic matrix of the beads is gradually degraded, the inorganic activity in the beads The substance is then released. Preparation method: Add the dispersion liquid dropwise to the polymer solution to obtain an electrospinning solution containing inorganic active components; perform electrospinning on the electrospinning solution, vacuum-dry the obtained electrospun fiber membrane, and then carry out epoxy After ethane treatment, a macroporous bone tissue engineering scaffold rich in inorganic active ingredients and having a composite structure of micron beads and nanofibers is finally obtained. The presence of beads will increase the pore size and surface roughness of the scaffold, which is conducive to the infiltration and growth of cells and osteogenic differentiation. The preparation method of the invention is simple, has a short process and is suitable for mass production.

Description

technical field [0001] The invention relates to a macroporous bone tissue engineering support capable of sustainably releasing inorganic active ingredients and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Treatment of critically sized bone tissue defects resulting from trauma, tumors, and congenital diseases remains a major clinical challenge. It is estimated that there are more than 30 million cases of bone repair that require clinical transplantation every year worldwide. Due to good osseointegration, osteoconductivity, and osteoinductivity, autologous / allograft transplantation remains the gold standard for the treatment of such diseases. However, the limited number of grafts, damage to the donor site, and potential cross-infection limit the clinical use of autologous / allogeneic transplantation. With the development of bone tissue engineering (BTE), scaffold-based tissue engineering approaches provide an opti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/44A61L27/56A61L27/58D01D5/00
CPCA61L27/446A61L27/46A61L27/56A61L27/58A61L2300/622A61L2400/12D01D5/0038
Inventor 刘来俊王富军张天添李超婧王璐张宇周俊泽
Owner DONGHUA UNIV