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A kind of hydrogel with ROS response and its preparation method and application

A technology of hydrogel and chitosan, which is applied in the field of hydrogel with ROS response and its preparation, can solve the problems of poor water solubility, poor adhesion, and narrowness of chitosan, and achieve poor water solubility and adhesion Poor sex and the effect of preventing restenosis

Active Publication Date: 2022-05-03
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned problems in the prior art, the present invention provides a ROS-responsive hydrogel and its preparation method and application, which introduces catechol groups, endows chitosan with a certain adhesion ability, and simultaneously acts as a fast Endothelialization, prevent restenosis, effectively solve the problems of poor water solubility and poor adhesion of chitosan

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  • A kind of hydrogel with ROS response and its preparation method and application
  • A kind of hydrogel with ROS response and its preparation method and application
  • A kind of hydrogel with ROS response and its preparation method and application

Examples

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Embodiment 1

[0044]A kind of hydrogel with ROS response, its preparation method comprises the following steps:

[0045] (1) Add catechol-modified chitosan into the Tris-base solution, stir magnetically until it is completely dissolved, and then stir for 1 hour under ice-bath conditions to obtain an intermediate solution;

[0046] (2) Dissolve cystamine dihydrochloride in the Tris-base solution, then dropwise add it to the intermediate solution obtained in step (1) under stirring conditions, continue stirring for 5 minutes, and then place it at a temperature of 37°C to form a gel to obtain ROS-responsive hydrogels.

[0047] Catechol-modified chitosan is prepared by the following method:

[0048] (1) Add chitosan to RO water, then add concentrated hydrochloric acid and stir until completely dissolved, adjust the pH value to 5 by using 1mol / L sodium hydroxide solution and 1mol / L hydrochloric acid solution to obtain a chitosan solution;

[0049] (2) Dissolve 3,4-dihydroxyphenylpropionic acid...

Embodiment 2

[0054] A kind of hydrogel with ROS response, its preparation method comprises the following steps:

[0055] (1) Add catechol-modified chitosan into the Tris-base solution, stir magnetically until it is completely dissolved, and then stir for 2 hours under ice bath conditions to obtain an intermediate solution;

[0056] (2) Dissolve cystamine dihydrochloride in the Tris-base solution, then dropwise add it to the intermediate solution obtained in step (1) under stirring conditions, continue stirring for 5 minutes, and then place it at a temperature of 37°C to form a gel to obtain ROS-responsive hydrogels.

[0057] Catechol-modified chitosan is prepared by the following method:

[0058] (1) Add chitosan to RO water, then add concentrated hydrochloric acid and stir until completely dissolved, adjust the pH value to 5 by using 1mol / L sodium hydroxide solution and 1mol / L hydrochloric acid solution to obtain a chitosan solution;

[0059] (2) Dissolve 3,4-dihydroxyphenylpropionic ac...

Embodiment 3

[0064] A kind of hydrogel with ROS response, its preparation method comprises the following steps:

[0065] (1) Add catechol-modified chitosan into the Tris-base solution, stir magnetically until it is completely dissolved, and then stir for 2 hours under ice bath conditions to obtain an intermediate solution;

[0066] (2) Dissolve cystamine dihydrochloride in the Tris-base solution, then dropwise add it to the intermediate solution obtained in step (1) under stirring conditions, continue stirring for 5 minutes, and then place it at a temperature of 37°C to form a gel to obtain ROS-responsive hydrogels.

[0067] Catechol-modified chitosan is prepared by the following method:

[0068] (1) Add chitosan to RO water, then add concentrated hydrochloric acid and stir until completely dissolved, adjust the pH value to 5 by using 1mol / L sodium hydroxide solution and 1mol / L hydrochloric acid solution to obtain a chitosan solution;

[0069] (2) Dissolve 3,4-dihydroxyphenylpropionic ac...

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Abstract

The invention provides a hydrogel with ROS response and its preparation method and application. During preparation, carbodiimide is used as a catalyst to graft catechol groups on chitosan macromolecules to improve the Solubility and adhesion of glycans. Then cystamine was used as a cross-linking agent, and its amino group reacted with benzoquinone formed after oxidation of catechol groups to undergo a Michael addition reaction to form a cross-linked network, thereby forming a hydrogel. The present invention also includes the hydrogel with ROS response prepared by the above method and its application. Among them, the disulfide bond contained in cystamine can be broken and transformed into sulfinic acid under the action of active oxygen, thereby destroying the structure of the hydrogel, and can play an intelligent role in atherosclerotic sites where more active oxygen species are produced. The role of drug release, avoiding the sudden release of drugs in the lesion, is expected to reduce the risk of intravascular restenosis, delayed endothelial healing and late thrombosis.

Description

technical field [0001] The invention belongs to the technical field of cardiovascular treatment, and in particular relates to a ROS-responsive hydrogel and its preparation method and application. Background technique [0002] The use of environment-responsive materials to control drug release is a current research hotspot, and it has been proven to have good effects in the field of targeted nanocarriers, various surface coatings, and hydrogels. In the research of environment-responsive hydrogels, the response mode is mainly reflected in the changes of quality, swelling ability and degradation ability according to the changes of the surrounding environment. Due to the presence of inflammation, the microenvironment of the atherosclerotic site has a weakly acidic pH (≈6.5, normal human body pH=7.4) and contains more reactive oxygen species, which makes it difficult for the preparation of intelligent environment-responsive hydrogels suitable for this environment. Glue provides ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K31/722A61K47/54A61P9/10C08J3/075C08B37/08C08L5/08
CPCA61K9/06A61K31/722A61K47/545A61K47/54A61P9/10C08J3/075C08B37/003C08J2305/08
Inventor 赵安莎刘力路槟阳杨苹
Owner SOUTHWEST JIAOTONG UNIV
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