Clostridium difficile-specific antigenic peptide

Abstract

The present invention relates to a specific antigenic peptide of Clostridium difficile, especially the specific antigenic peptide for highly toxigenic Clostridium difficile RT027, which comprises the amino acid sequence shown in SEQ ID No.1. Using the specific peptides and specific antibodies of the present invention, a new detection kit for high-yielding Clostridium difficile strains and a Clostridium difficile vaccine can be developed, which is of great significance for the detection and treatment of high-yielding Clostridium difficile strain infection.

Description

technical field

[0001] The present invention relates to a specific antigen peptide of Clostridium difficile. Background technique

[0002] Clostridium difficile ( Clostridioides difficile , CD) is a Gram-positive, spore-producing obligate anaerobe. CD is the main pathogen of antibiotic-associated diarrhea (AAD) in hospitals, which can cause mild to severe diarrhea, colitis, pseudomembranous colitis, toxic megacolon, etc., and even death in severe cases. In recent years, the emergence of highly toxin-producing Clostridium difficile (RT027 / NAP1 / BI / ST01) has led to a gradual increase in the morbidity and mortality of Clostridiodes difficile infection (CDI), causing tens of thousands of deaths worldwide each year. Costs run into the billions of dollars.

[0003] In 2013, the CDC (Center for Disease Control and Prevention) listed Clostridium difficile infection at the top of the urgency level of "microorganisms that pose a public health threat". The pathogenicity of C. diffic...

Examples

Embodiment Construction

[0017] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0018] 1. Cultivation of Clostridium difficile

[0019] The present invention selects 4 high toxigenic Clostridium difficile RT027 strains (RT027 / NAP1 / BI / ST01) and 3 low toxigenic Clostridium difficile RT017 strains for bacterial culture. Before using the strains, the cryopreserved strains were thawed at room temperature, inoculated into CDMN selective medium, and placed in an anaerobic tank at 37°C for 48-72 hours after the three-division line. A single colony was selected and transformed into CDMN culture medium for ...

technical field

[0001] The present invention relates to a specific antigen peptide of Clostridium difficile. Background technique

[0002] Clostridium difficile ( Clostridioides difficile , CD) is a Gram-positive, spore-producing obligate anaerobe. CD is the main pathogen of antibiotic-associated diarrhea (AAD) in hospitals, which can cause mild to severe diarrhea, colitis, pseudomembranous colitis, toxic megacolon, etc., and even death in severe cases. In recent years, the emergence of highly toxin-producing Clostridium difficile (RT027 / NAP1 / BI / ST01) has led to a gradual increase in the morbidity and mortality of Clostridiodes difficile infection (CDI), causing tens of thousands of deaths worldwide each year. Costs run into the billions of dollars.

[0003] In 2013, the CDC (Center for Disease Control and Prevention) listed Clostridium difficile infection at the top of the urgency level of "microorganisms that pose a public health threat". The pathogenicity of C. diffic...

Examples

Embodiment Construction

[0017] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0018] 1. Cultivation of Clostridium difficile

[0019] The present invention selects 4 high toxigenic Clostridium difficile RT027 strains (RT027 / NAP1 / BI / ST01) and 3 low toxigenic Clostridium difficile RT017 strains for bacterial culture. Before using the strains, the cryopreserved strains were thawed at room temperature, inoculated into CDMN selective medium, and placed in an anaerobic tank at 37°C for 48-72 hours after the three-division line. A single colony was selected and transformed into CDMN culture medium for ...

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