Application of persimmon leaf extract in treatment of insomnia
A technology of persimmon leaf extract and sleep, applied in the field of medicine, can solve problems such as complex composition of active substances, complex causes of insomnia, and unclear mechanism of treatment of insomnia
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[0046] Example 1 Effect of persimmon leaf extract of the present invention on mice falling asleep caused by sodium pentobarbital 1. Experimental animals
[0047] 60 healthy Kunming mice, clean grade, half male and half female, weighing 18-22 g. Experimental animal license number: SCXK (Beijing) 2014-0004; the mice were adapted to the feeding environment for one week before the experiment, and all mice were kept in a normal-grade experimental animal room with a temperature of 21±2°C and followed the circadian rhythm. Mice had free access to food and water.
[0048] 2. Experimental Drugs
[0049] Both persimmon leaf extract (NXQ) and alprazolam were prepared using 0.5% sodium carboxymethylcellulose as a solvent, and the drugs should be fully vortexed before administration.
[0050] Preparation of Persimmon Leaf Extract (NXQ): Dissolve 40mg of Persimmon Leaf Extract in 10ml of 0.5% Sodium Carboxymethyl Cellulose to prepare a 4mg / ml stock solution. liquid. For example, if the...
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