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Antigen peptide combination related to liver cancer driver gene mutation and application thereof

一种抗原肽、肝癌的技术，应用在生物医药领域，能够解决未能形成效果、优越治疗剂等问题，达到抑制肿瘤细胞生长、良好临床转化及疾病预防前景的效果

Pending Publication Date: 2022-03-11
SHENZHEN NEW TARGET BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, due to the need for multidisciplinary, high-tech, and multi-platform collaborative research, the antigenic peptides related to liver cancer driver gene mutations have not been systematically discovered and a relatively complete peptide library has not been established, so it has not yet formed a superior effect. therapeutic agents, etc.

Method used

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  • Antigen peptide combination related to liver cancer driver gene mutation and application thereof

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Experimental program
Comparison scheme
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Embodiment Construction

[0020] Below, in conjunction with specific embodiment, the present invention is described further:

[0021] Antigen peptides related to liver cancer driver gene mutations used in the examples are shown in Table 1:

[0022] Table 1 Antigen peptide sequences related to liver cancer driver gene mutations

[0023]

[0024] The operation for the establishment of antigenic peptide-specific CTL clones related to liver cancer driver gene mutations is as follows:

[0025] 10 from the same healthy donor 5 CD8 + T cells by loading 10 antigenic peptides associated with liver cancer driver gene mutations 4 Mo-DCs were stimulated twice at intervals of 1 week, and then passed autologous 10 5 Mitomycin C-treated PBMCs loaded with antigenic peptides associated with liver cancer driver gene mutations were stimulated once and screened by standard cytotoxicity assays.

[0026] T2 cells were loaded with 5uM antigen peptides related to liver cancer driver gene mutations as target cells, and...

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Abstract

The invention discloses an antigen peptide combination related to liver cancer driver gene mutation. The liver cancer driver genes are NFE2L2, PREX2 and ZFHX3; the sequences of the antigen peptide combination are as shown in SEQ ID NO.7, SEQ ID NO.14 and SEQ ID NO.15; according to the application of the antigen peptide combination, the antigen peptide combination is used for inducing generation of specific cytotoxic T cell clone; the antigen peptide combination has high affinity with MHC I molecules on DC cells, can effectively stimulate and induce generation of specific cytotoxic T lymphocytes, can be used for immune clearance of tumor cells with driver gene mutation related to liver cancer, and has good treatment potential.

Description

[0001] This application is a divisional application with application number 202110594226.9, application date of May 28, 2021, and the title of the invention "an antigenic peptide related to liver cancer driver gene mutation and its application". technical field [0002] The invention relates to an antigen peptide combination related to liver cancer driver gene mutation and application thereof, belonging to the technical field of biomedicine. Background technique [0003] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. Primary hepatic malignant tumors originate from the epithelial or mesenchymal tissues of the liver. The former is called primary liver cancer. [0004] Treatment methods include surgery, hepatic artery ligation, hepatic arterial chemoembolization, radiofrequency, cryotherapy, laser, microwave, as well as chemotherapy and radiation therapy. Biological therapy and traditional Chinese medicine are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N5/0783A61K39/00A61P35/00
CPCA61K39/0011A61P35/00A61K2039/5154C07K14/47C12N5/0636
Inventor 郝冰娜李许峰蔡睿赵环罗夫辛克纳吉张积仁
Owner SHENZHEN NEW TARGET BIOTECHNOLOGY CO LTD
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