Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for auxiliary diagnosis of tubercular meningitis as well as application and kit thereof

A technology of tuberculous meningitis and molecular markers, which is applied in the field of disease diagnosis, can solve problems such as the inability to detect cerebrospinal fluid samples, and improve the effect of diagnosis

Pending Publication Date: 2022-04-19
BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV +1
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the miRNAs that can be used for the detection of peripheral blood samples cannot be directly used for the detection of cerebrospinal fluid samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for auxiliary diagnosis of tubercular meningitis as well as application and kit thereof
  • Molecular marker for auxiliary diagnosis of tubercular meningitis as well as application and kit thereof
  • Molecular marker for auxiliary diagnosis of tubercular meningitis as well as application and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In order to identify differential miRNAs for distinguishing tuberculous meningitis from other infectious meningitis, 11 patients with tuberculous meningitis and 12 patients with other infectious meningitis (including 7 patients with viral meningitis, 3 Genome-wide miRNA microarray analysis was performed on the cerebrospinal fluid of 1 patient with bacterial meningitis and 2 patients with cryptococcal meningitis, and then the original sample data was standardized to obtain the fold difference value (Fold Change), with Fold Change>1.5 and P< 0.05 screened out 7 differential miRNAs between the two groups. The results are shown in Table 1.

[0034] TBM (tuberculous meningitis) group: (1) The symptoms and signs of meningitis in patients include one or more of the following: headache, irritability, vomiting, fever, neck stiffness, convulsions, focal neurological dysfunction, altered consciousness or drowsiness. (2) Positive acid-fast staining of cerebrospinal fluid, or posi...

Embodiment 2

[0039] Real-time fluorescent quantitative PCR (qRT-PCR) to verify differential miRNA

[0040](1) Reagents: miRNA chip: Agilent human miRNA chip (8*60K) V21 chip; internal reference gene for qPCR: Cel-miR-39; reagents for RNA extraction, reverse transcription and qPCR detection are commercial reagents (QIAGEN).

[0041] (2) Internal reference gene for qPCR: Cel-miR-39. Internal reference and target primers: See the table below for the upstream primers, and the downstream primers are universal primers (downstream primer sequence: 5'-CTCAACTGGTGTCGTGGA-3')

[0042] miRNA upstream primer Cel-miR-39 5'-GCCGAGAGCTGATTTCGTCT-3' hsa-miR-21-5p 5'-GCCGAGTAGCTTTATCAGACTG-3' hsa-miR-1587 5'-TCGGCAGGTTGGGCTGGGCT-3' hsa-miR-3152-3p 5'-GCCGAGTGTGTTAGAATAGGG-3' hsa-miR-7975 5'-TCGGCAGGATCCTAGTCAC-3'

[0043] (3) Instrument: ABI Quantistudio7.

[0044] The results are shown in Table 2 and Table 3.

[0045] Table 2. qPCR detection raw data (...

Embodiment 3

[0050] The inventors tried to combine differentially expressed miRNAs to improve specificity and sensitivity.

[0051] Table 3. qPCR detection miRNA combination (predicted probability)

[0052]

[0053]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of disease diagnosis, in particular to a molecular marker for auxiliary diagnosis of tubercular meningitis as well as application and a kit thereof. The invention relates to a molecular marker for auxiliary diagnosis of tubercular meningitis. The molecular marker is miR-1587; the sequence of the hsa-miR-1587 is as shown in SEQ ID NO. 1 (sequence identifier number). The invention has the advantages that: (1) the cost is low; (2) the sensitivity is high; and (3) the specificity is good.

Description

technical field [0001] The invention relates to the technical field of disease diagnosis, in particular to a molecular marker for auxiliary diagnosis of tuberculous meningitis and its application and kit. Background technique [0002] The early clinical manifestations and imaging changes of patients with tuberculous meningitis are not specific, and the low sensitivity of current laboratory diagnostic tests makes the clinical diagnosis of tuberculous meningitis still obviously challenging. The detection of Mycobacterium tuberculosis in cerebrospinal fluid is the gold standard for the diagnosis of tuberculous meningitis, but the positive rate of Ziehl-Neelsen staining in traditional cerebrospinal fluid smears is low (10-20%), and the culture time of Mycobacterium tuberculosis in cerebrospinal fluid is long (6-8 weeks) and not sensitive enough (sensitivity ranged from 36% to 81.8%). [0003] The World Health Organization recommends the GeneXpert MTB / RIF test as a diagnosis in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/689C12Q1/6851C12N15/11C12R1/32
CPCC12Q1/6883C12Q1/689C12Q1/6851C12Q2600/178C12Q2600/158C12Q2600/166C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 潘丽萍谌蒙蒙董静史雨婷张宗德孙琦贾红彦魏荣荣杜博平邢爱英
Owner BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com