Novel immunochromatography detection device

Abstract

The invention provides a novel immunochromatography detection device which comprises a sample pretreatment part, and when the device is used, a sample does not need to be subjected to pretreatment such as dilution or splitting decomposition, and one-step detection can be realized. The immunochromatography detection device provided by the invention is particularly suitable for rapid detection of the novel coronavirus SARS-CoV-2, sample collection can be carried out by adopting a mode of directly sampling a saliva sample in an oral cavity, self-sampling and self-detection of a patient are facilitated, positive and negative properties of the novel coronavirus can be diagnosed by a one-step method, and the immunochromatography detection device is convenient to use, simple, efficient and low in cost. The risk that viruses are spread to medical staff and health care workers is reduced, utilization of personal protection equipment is reduced, and the method is suitable for early-stage rapid diagnosis of infection conditions of the novel coronavirus by various medical institutions or individuals.

Description

[0001] This application is a divisional application with the application number 2020112540542, the application date is November 11, 2020, and the invention title is "A Novel Immunochromatographic Detection Device". technical field [0002] The invention belongs to the technical field of biological detection, and relates to a novel immunochromatographic detection device, in particular to a one-step immunochromatographic detection device including pretreatment for detecting novel coronaviruses. Background technique [0003] At present, the detection of new coronaviruses is mainly based on PCR-based viral nucleic acid detection. The detection principle is to use the unique gene sequence of the virus as the detection target, and through PCR amplification, the target DNA sequence we selected will increase exponentially. The more the target gene is amplified, the stronger the fluorescent signal will be accumulated. In samples without viruses, no increase in fluorescent signal coul...

AI Technical Summary

Problems solved by technology

In samples without virus, no increase in fluorescent signal could be detected due to no amplification of the target gene

The detection of new coronavirus by nucleic acid has high specificity and sensitivity, but the detection method has high technical requirements, the specimen needs special treatment, and requires professional equipment such as PCR amplifier and gel electrophoresis. The test takes a long time, requires professional technicians to operate and judge the test results, and cannot be applied to the early preliminary screening of community hospitals, customs, CDC and other grassroots

[0004] At present, Chinese patent application 202010184358.X has disclosed a fluorescent immunochromatographic device for detecting COVID-19 and its use method for the above problems, and Chinese patent application 202010184382 has disclosed a colloidal gold immunochromatographic device for detecting COVID-19. The method of use, the corresponding product can be applied to the early primary screening at the grassroots level, but it still has certain operational defects, especially the method of detecting virus antigens needs to be diluted or lysed for the first time to further detect the collected samples, that is, using Two-step detection method, collect samples from the patient's oropharynx or nasopharynx, after sampling, process the collected samples in other auxiliary devices other than the detection device, and then add the processed samples to the detection device for result detection

This kind of sampling may increase the risk of virus transmission to medical staff and health care workers who lack adequate personal protective equipment. The operation steps are not the most simplified, which is easy to cause operational errors, which will have a certain impact on the test results.

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