Rapid and efficient tobacco hairy root induction and propagation method
A hairy root and tobacco technology, applied in the field of tobacco hairy root induction and propagation, can solve the problems of high cost, demand, unfavorable research and production of sterile seedlings, and achieve convenient material collection, fast induction speed, high-efficiency induction and multiplication effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Step 1: Infect the tobacco explants of soil cultured seedlings with wild-type A4 Agrobacterium rhizogenes infection solution.
[0039] Specifically, the explants of 4-week-old tobacco soil cultured seedlings were cut into leaves of 1 × 1 cm, that is, the leaves of tobacco soil cultured seedlings were added to the invasion dye solution for 5 minutes. It should be noted that the invasion process The dye solution was centrifuged with 50 mL of Agrobacterium rhizogenes with an OD value (600) of 0.8, and resuspended with an equal volume of MS liquid.
[0040] Step 2: In this example, under the condition of 25°C, the infected explants were inoculated on the co-cultivation medium for 48 hours in the dark. The co-culture medium was MS medium, and the dark culture was carried out After 48 h, at this point, the explants differentiated to form hairy roots.
[0041] Step 3: Transfer the dark-cultured explants to the hairy root induction medium for induction culture, cut off 3 cm of...
Embodiment 2
[0045] Step 1: Infect the tobacco explants of soil cultured seedlings with wild-type R1000 Agrobacterium rhizogenes infection solution.
[0046] Specifically, the explants of 4-week-old tobacco soil cultured seedlings were cut into leaves of 1 × 1 cm, that is, the leaves of tobacco soil cultured seedlings were added to the invasion dye solution for 10 minutes. It should be noted that the invasion process The dye solution was centrifuged with 50 mL of Agrobacterium rhizogenes with an OD value (600) of 0.8, and resuspended with an equal volume of MS liquid.
[0047] Step 2: In this example, under the condition of 25°C, the infected explants were inoculated on the co-cultivation medium for 24 hours in dark, and the co-culture medium was MS medium, and the dark culture was carried out 24 years later, at this point, the explants differentiated to form hairy roots.
[0048] Step 3: Transfer the dark cultured explants to the hairy root induction medium for induction culture, cut off...
Embodiment 3
[0052] Step 1: Infect the tobacco explants of soil cultured seedlings with wild-type R1601 Agrobacterium rhizogenes infection solution.
[0053] Specifically, the explants of 4-week-old tobacco soil cultured seedlings were cut into leaves of 1 × 1 cm, that is, the leaves of tobacco soil cultured seedlings were added to the invasion dye solution for 10 minutes. It should be noted that the invasion process The dye solution was centrifuged with 50 mL of Agrobacterium rhizogenes with an OD value (600) of 0.8, and resuspended with an equal volume of MS liquid.
[0054] Step 2: In this example, under the condition of 25°C, the infected explants were inoculated on the co-cultivation medium for 48 hours in the dark. The co-culture medium was MS medium, and the dark culture was carried out After 48 h, at this point, the explants differentiated to form hairy roots.
[0055] Step 3: Transfer the dark-cultured explants to the hairy root induction medium for induction culture, cut off 2-3...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More