Composite composition containing polypeptide and plant extract and preparation method thereof
Through the dual mixing and microfluidic process combining dynamic network structure and molecular chain entanglement, the stability problem of complex plant extract liquid preparations is solved, and the stability and multiple functional effects of the product during storage are achieved.
Patent Information
- Application Number
- CN202411407949.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-10
- Publication Date
- 2025-01-03
- Estimated Expiration
- Not applicable · inactive patent
Smart Images
Figure CN119235009A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of plant extracts, and more specifically, to a composite composition containing polypeptides and plant extracts and a preparation method thereof. Background Art
[0003] Traditional plant extract products are made by simply treating and processing Chinese medicinal materials, so there are many problems in terms of technical content and quality control. Later, with the development of technology, water extraction, alcohol extraction, fermentation, carbon dioxide supercritical extraction and other technologies were gradually developed, which greatly improved the purity and quality of plant extracts. In addition, technicians have also conducted detailed animal and human experiments on the main functional components of various plant extracts, and clarified the physiological mechanism of action of some functional components of plant extracts, such as the medicinal value and health value of active ingredients such as plant polyphenols, flavonoids, saponins, polysaccharides, alcohols, and plant alkaloids.
[0004] Currently, there are many forms of plant extract preparations, which can be roughly divided into micropowders, liquids, microcapsules, tablets, etc. Among them, liquid preparations have better absorption effects and can be compounded with plant extracts with various functions. They can also be compounded with other types of components such as peptides, proteins, biological agents, etc. to obtain health products with multiple functions.
[0005] For the above-mentioned compound plant extract liquid preparations, due to the different physical and chemical properties between different types of plant extracts, as well as the different compatibility between other components and plant extracts, the storage stability, component stability, compatibility and other performance of the liquid preparations deteriorate. Therefore, how to improve the stability of multi-component compound plant extract preparations is a difficult problem that needs to be solved urgently. Summary of the invention
[0006] In order to further improve the stability of the composite plant extract liquid preparation, the present application provides a composite composition containing a polypeptide and a plant extract and a preparation method thereof.
[0007] In a first aspect, the present application provides a composite composition containing a polypeptide and a plant extract and a preparation method thereof, using the following technical scheme:
[0008] A method for preparing a composite composition containing a polypeptide and a plant extract, characterized in that it comprises the following steps:
[0009] 1) mixing plant extract, pyrroloquinoline quinone, oligofructose, fermented mulberry juice, fermented polygonatum powder, vitamin B6, vitamin B12, citric acid, pectin and the first stable phase to obtain pre-liquid A;
[0010] 2) Evenly mixing the oyster peptide, the sea cucumber peptide, the earthworm protein peptide and the second stable phase to prepare a pre-liquid B;
[0011] 3) adding water into the container, heating it to 80-90° C., adding pre-liquid A and pre-liquid B into the water using a microfluidic process, adding hydroxyproline while stirring, and continuing to mix for a period of time after all materials are added, placing it in a cold trap and rapidly cooling it to room temperature, and standing it to obtain a composite composition containing polypeptides and plant extracts;
[0012] The first stable phase includes carrageenan and aldehyde-modified polysaccharide;
[0013] The second stable phase includes polar group functionalized chitosan and hydroxytyrosol.
[0014] Preferably, the step 1) further includes one or more of the following technical features:
[0015] 11) The plant extract is obtained by fermenting and extracting Cistanche deserticola, American ginseng, Cornus officinalis, Lycium barbarum, and Eucommia ulmoides male pollen;
[0016] 12) The fermented polygonatum powder is prepared by fermenting polygonatum, platycodon grandiflorum, green tea and oligofructose.
[0017] Preferably, the aldehyde-modified polysaccharide is one or more of aldehyde-modified seaweed polysaccharide, aldehyde-modified pullulan polysaccharide and aldehyde-modified pectin polysaccharide.
[0018] Preferably, the polar group functionalized chitosan is one or more of polyethylene glycol functionalized chitosan, carboxymethyl chitosan, and thiolated chitosan.
[0019] Preferably, the first stable phase accounts for 2-3% of the total mass of the pre-liquid A.
[0020] Preferably, the second stable phase accounts for 3.5-5% of the total mass of the pre-liquid B.
[0021] Preferably, in step 3), the pre-fluid A and pre-fluid B are added to water using a microfluidic process: pre-fluid A is added to water at a rate of 50-65 mL / min; pre-fluid B is added to water at a rate of 80-120 mL / min.
[0022] Preferably, in step 3), the amount of hydroxyproline added is 0.25-0.35% of the total material mass.
[0023] Preferably, in step 3), the rate of addition of hydroxyproline is greater than the rate of addition of pre-liquid A, and less than the rate of addition of pre-liquid B.
[0024] In a second aspect, the present application provides a composite composition containing a polypeptide and a plant extract, which is prepared by the above-mentioned preparation method.
[0025] In summary, this application has the following beneficial effects:
[0026] 1. The present application adopts a double mixing method, firstly mixing components such as plant extracts, fermented mulberry juice, fermented polygonatum powder with the first stable phase to prepare preliquid A, and then mixing components such as biological peptides such as oyster peptides and sea cucumber peptides with the second stable phase to prepare preliquid B, wherein the double helix molecular structure of carrageenan in the first stable phase can form a stable dynamic network dispersion form in the preliquid A, thereby improving the dispersion uniformity between components such as plant extracts; the polar group functionalized chitosan in the second stable phase can form molecular chain entanglement with components such as biological peptides such as oyster peptides, thereby reducing the probability of instability. Then, the pre-liquid A and the pre-liquid B are mixed under higher temperature conditions, so that the plant extract, fermented Polygonatum sibiricum powder and other components in the pre-liquid A are evenly dispersed and fused with the biological peptide components in the pre-liquid B. Finally, when the temperature is lowered, the aldehyde-modified polysaccharide in the first stable phase forms a dynamic Schiff base bond with the polar group functionalized chitosan. At the same time, with the assistance of hydroxyproline, hydrogen bonds are formed between hydroxytyrosol and carrageenan and some component molecules in the pre-liquid A and pre-liquid B, thereby fixing this uniform stable system. In the subsequent storage process, the product characteristics and dispersion characteristics can be maintained for a long time, and the undesirable phenomena of stratification, turbidity and precipitation are not easy to occur.
[0027] 2. The plant extracts of the present application are obtained by fermentation and extraction of Cistanche deserticola, American ginseng, Cornus officinalis, Lycium barbarum, and Eucommia ulmoides male pollen, among which Cistanche deserticola can strengthen yin and replenish qi, protect the kidney and liver; American ginseng can replenish qi and nourish yin, consolidate the kidney and preserve essence; Lycium barbarum and Cornus officinalis can replenish yang and replenish vital energy; in addition, fermented Polygonatum sibiricum powder is obtained by fermentation of Polygonatum sibiricum, Platycodon grandiflorum and green tea, and has the effects of moistening the lungs, relieving sore throat, nourishing yin and replenishing qi; biological peptides have the effects of strengthening the foundation and nourishing the essence, and resisting fatigue. These components with different functions can produce multiple functional effects and reflect better health care effects. Moreover, by adopting the stabilization process of the present application, components with different biochemical characteristics can be stably compounded together, and they are not easy to become unstable or react with each other.
[0028] 3. This application further improves the stability of the entire system by optimizing and adjusting the types and compositions of aldehyde-modified polysaccharides and polar group-functionalized chitosan, as well as the amounts of the first stable phase and the second stable phase added, and testing and screening the microfluidic process conditions of the two. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] Figure 1 It is a schematic diagram of the stability test results of the composite compositions containing polypeptides and plant extracts of Examples 1-3 and Comparative Examples 1-3 of the present application. DETAILED DESCRIPTION
[0030] The present application is further described in detail below with reference to the embodiments.
[0031] Unless otherwise specified, the raw materials used in the examples and comparative examples of the present application are all commercially available.
[0032] Example
[0033] Example 1
[0034] The preparation method of the composite composition containing polypeptides and plant extracts of this embodiment comprises the following steps:
[0035] 1) 300 g of plant extract, 30 g of pyrroloquinoline quinone, 50 g of oligofructose, 200 g of fermented mulberry juice, 100 g of fermented polygonatum powder, 5 g of vitamin B6, 5 g of vitamin B12, 20 g of citric acid, 100 g of pectin, and 25 g of the first stable phase were uniformly mixed to prepare a pre-liquid A;
[0036] 2) 60 g of oyster peptide, 30 g of sea cucumber peptide, 30 g of earthworm protein peptide and 12 g of the second stable phase were mixed to prepare pre-liquid B;
[0037] 3) Add water into the container, raise the temperature to 80° C., add 1000 mL of pre-liquid A and 1000 mL of pre-liquid B into the water at a rate of 80 mL / min using a microfluidic process, add 70 g of hydroxyproline at a rate of 20 mL / min while stirring, continue mixing for a period of time after all the materials are added, put into a cold trap and quickly cool to room temperature, and stand to obtain a composite composition containing polypeptides and plant extracts.
[0038] The first stable phase is composed of carrageenan and aldehyde-modified polysaccharide in a mass ratio of 1:2.5, and the aldehyde-modified polysaccharide is aldehyde-modified sodium alginate. The second stable phase is composed of polar group functionalized chitosan and hydroxytyrosol in a mass ratio of 3:1, and the polar group functionalized chitosan is carboxymethyl chitosan.
[0039] The plant extract of this embodiment is prepared by the following steps: 300g of Cistanche deserticola, 500g of American ginseng, 1.5kg of Cornus officinalis, 3kg of Lycium barbarum, 150g of Eucommia male pollen, and 50kg of purified water are mixed evenly in a fermentation tank, and then Lactobacillus plantarum BXM2 is added to obtain the plant extract through fermentation and extraction.
[0040] The fermented polygonatum powder of the present embodiment is prepared by the following steps: 600g polygonatum, 350g platycodon, 200g green tea, 500g oligofructose, and 10kg purified water are mixed evenly in a fermentation tank, and then Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus, and yeast are added to obtain the powder through fermentation and extraction.
[0041] Example 2
[0042] The preparation method of the composite composition containing polypeptides and plant extracts of this embodiment comprises the following steps:
[0043] 1) 300 g of plant extract, 30 g of pyrroloquinoline quinone, 50 g of oligofructose, 200 g of fermented mulberry juice, 100 g of fermented polygonatum powder, 5 g of vitamin B6, 5 g of vitamin B12, 20 g of citric acid, 100 g of pectin, and 17 g of the first stable phase were uniformly mixed to prepare a pre-liquid A;
[0044] 2) 60 g of oyster peptide, 30 g of sea cucumber peptide, 30 g of earthworm protein peptide and 18 g of the second stable phase were mixed to prepare pre-liquid B;
[0045] 3) Add water into the container, raise the temperature to 90° C., add 1000 mL of pre-liquid A into the water at a rate of 65 mL / min by using a microfluidic process; add 1000 mL of pre-liquid B into the water at a rate of 80 mL / min, add 50 g of hydroxyproline at a rate of 30 mL / min while stirring, continue mixing for a period of time after all the materials are added, put into a cold trap and quickly cool to room temperature, and stand to obtain a composite composition containing polypeptides and plant extracts.
[0046] The first stable phase is composed of carrageenan and aldehyde-modified polysaccharide in a mass ratio of 1:2.5, and the aldehyde-modified polysaccharide is aldehyde-modified pectin polysaccharide. The second stable phase is composed of polar group functionalized chitosan and hydroxytyrosol in a mass ratio of 3:1, and the polar group functionalized chitosan is thiolated chitosan.
[0047] The plant extract of this embodiment is prepared by the following steps: 300g of Cistanche deserticola, 500g of American ginseng, 1.5kg of Cornus officinalis, 3kg of Lycium barbarum, 150g of Eucommia male pollen, and 50kg of purified water are mixed evenly in a fermentation tank, and then Lactobacillus plantarum BXM2 is added to obtain the plant extract through fermentation and extraction.
[0048] The fermented polygonatum powder of the present embodiment is prepared by the following steps: 600g polygonatum, 350g platycodon, 200g green tea, 500g oligofructose, and 10kg purified water are mixed evenly in a fermentation tank, and then Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus, and yeast are added to obtain the powder through fermentation and extraction.
[0049] Example 3
[0050] The preparation method of the composite composition containing polypeptides and plant extracts of this embodiment comprises the following steps:
[0051] 1) 300 g of plant extract, 30 g of pyrroloquinoline quinone, 50 g of oligofructose, 200 g of fermented mulberry juice, 100 g of fermented polygonatum powder, 5 g of vitamin B6, 5 g of vitamin B12, 20 g of citric acid, 100 g of pectin, and 20 g of the first stable phase are uniformly mixed to prepare a pre-liquid A;
[0052] 2) 60 g of oyster peptide, 30 g of sea cucumber peptide, 30 g of earthworm protein peptide and 15 g of the second stable phase were mixed to prepare pre-liquid B;
[0053] 3) Add water into the container, raise the temperature to 85° C., add 1000 mL of pre-liquid A into the water at a rate of 50 mL / min using a microfluidic process; add 1000 mL of pre-liquid B into the water at a rate of 120 mL / min, add 65 g of hydroxyproline at a rate of 60 mL / min while stirring, continue mixing for a period of time after all the materials are added, put into a cold trap and quickly cool to room temperature, and stand to obtain a composite composition containing polypeptides and plant extracts.
[0054] The first stable phase is composed of carrageenan and aldehyde-modified polysaccharide in a mass ratio of 1:2.5, and the aldehyde-modified polysaccharide is aldehyde-modified pullulan. The second stable phase is composed of polar group functionalized chitosan and hydroxytyrosol in a mass ratio of 3:1, and the polar group functionalized chitosan is polyethylene glycol functionalized chitosan.
[0055] The plant extract of this embodiment is prepared by the following steps: 300g of Cistanche deserticola, 500g of American ginseng, 1.5kg of Cornus officinalis, 3kg of Lycium barbarum, 150g of Eucommia male pollen, and 50kg of purified water are mixed evenly in a fermentation tank, and then Lactobacillus plantarum BXM2 is added to obtain the plant extract through fermentation and extraction.
[0056] The fermented polygonatum powder of the present embodiment is prepared by the following steps: 600g polygonatum, 350g platycodon, 200g green tea, 500g oligofructose, and 10kg purified water are mixed evenly in a fermentation tank, and then Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus, and yeast are added to obtain the powder through fermentation and extraction.
[0057] Comparative Example 1
[0058] The preparation method of the composite composition containing polypeptide and plant extract of this comparative example comprises the following steps:
[0059] 1) 300 g of plant extract, 30 g of pyrroloquinoline quinone, 50 g of oligofructose, 200 g of fermented mulberry juice, 100 g of fermented polygonatum powder, 5 g of vitamin B6, 5 g of vitamin B12, 20 g of citric acid, and 100 g of pectin were uniformly mixed to prepare pre-liquid A;
[0060] 2) 60 g of oyster peptide, 30 g of sea cucumber peptide and 30 g of earthworm protein peptide were mixed evenly to prepare pre-liquid B;
[0061] 3) Add water into the container, raise the temperature to 80° C., add 1000 mL of pre-liquid A and 1000 mL of pre-liquid B into the water at a rate of 80 mL / min using a microfluidic process, add 70 g of hydroxyproline at a rate of 20 mL / min while stirring, continue mixing for a period of time after all the materials are added, put into a cold trap and quickly cool to room temperature, and stand to obtain a composite composition containing polypeptides and plant extracts.
[0062] The plant extract of this comparative example is prepared by the following steps: 300g of Cistanche deserticola, 500g of American ginseng, 1.5kg of Cornus officinalis, 3kg of Lycium barbarum, 150g of Eucommia male pollen, and 50kg of purified water are mixed evenly in a fermentation tank, and then Lactobacillus plantarum BXM2 is added to obtain the plant extract through fermentation and extraction.
[0063] The fermented polygonatum powder of this comparative example is prepared by the following steps: 600g polygonatum, 350g platycodon, 200g green tea, 500g oligofructose and 10kg pure water are mixed evenly in a fermentation tank, and then Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus and yeast are added to obtain the powder through fermentation and extraction.
[0064] Comparative Example 2
[0065] The preparation method of the composite composition containing polypeptide and plant extract of this comparative example comprises the following steps:
[0066] 1) 300 g of plant extract, 30 g of pyrroloquinoline quinone, 50 g of oligofructose, 200 g of fermented mulberry juice, 100 g of fermented polygonatum powder, 5 g of vitamin B6, 5 g of vitamin B12, 20 g of citric acid, 100 g of pectin, and 25 g of the first stable phase were uniformly mixed to prepare a pre-liquid A;
[0067] 2) 60 g of oyster peptide, 30 g of sea cucumber peptide, 30 g of earthworm protein peptide and 12 g of the second stable phase were mixed to prepare pre-liquid B;
[0068] 3) Add water into the container, raise the temperature to 80° C., add 1000 mL of pre-liquid A and 1000 mL of pre-liquid B into the water at a rate of 80 mL / min using a microfluidic process, add 70 g of hydroxyproline at a rate of 20 mL / min while stirring, continue mixing for a period of time after all the materials are added, put into a cold trap and quickly cool to room temperature, and stand to obtain a composite composition containing polypeptides and plant extracts.
[0069] The first stable phase is carrageenan and the second stable phase is hydroxytyrosol.
[0070] The plant extract of this comparative example is prepared by the following steps: 300g of Cistanche deserticola, 500g of American ginseng, 1.5kg of Cornus officinalis, 3kg of Lycium barbarum, 150g of Eucommia male pollen, and 50kg of purified water are mixed evenly in a fermentation tank, and then Lactobacillus plantarum BXM2 is added to obtain the plant extract through fermentation and extraction.
[0071] The fermented polygonatum powder of this comparative example is prepared by the following steps: 600g polygonatum, 350g platycodon, 200g green tea, 500g oligofructose and 10kg pure water are mixed evenly in a fermentation tank, and then Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus and yeast are added to obtain the powder through fermentation and extraction.
[0072] Comparative Example 3
[0073] The preparation method of the composite composition containing polypeptide and plant extract of this comparative example comprises the following steps:
[0074] 1) 300 g of plant extract, 30 g of pyrroloquinoline quinone, 50 g of oligofructose, 200 g of fermented mulberry juice, 100 g of fermented polygonatum powder, 5 g of vitamin B6, 5 g of vitamin B12, 20 g of citric acid, 100 g of pectin, and 25 g of the first stable phase were uniformly mixed to prepare a pre-liquid A;
[0075] 2) 60 g of oyster peptide, 30 g of sea cucumber peptide, 30 g of earthworm protein peptide and 12 g of the second stable phase were mixed to prepare pre-liquid B;
[0076] 3) Add water into the container, raise the temperature to 80° C., add 1000 mL of pre-liquid A and 1000 mL of pre-liquid B into the water at a rate of 80 mL / min using a microfluidic process, continue mixing for a period of time after all the materials are added, put into a cold trap and quickly cool to room temperature, and stand to obtain a composite composition containing polypeptides and plant extracts.
[0077] Wherein, the first stable phase and the second stable phase are both ethylene glycol.
[0078] The plant extract of this comparative example is prepared by the following steps: 300g of Cistanche deserticola, 500g of American ginseng, 1.5kg of Cornus officinalis, 3kg of Lycium barbarum, 150g of Eucommia male pollen, and 50kg of purified water are mixed evenly in a fermentation tank, and then Lactobacillus plantarum BXM2 is added to obtain the plant extract through fermentation and extraction.
[0079] The fermented polygonatum powder of this comparative example is prepared by the following steps: 600g polygonatum, 350g platycodon, 200g green tea, 500g oligofructose and 10kg pure water are mixed evenly in a fermentation tank, and then Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus and yeast are added to obtain the powder through fermentation and extraction.
[0080] Performance testing
[0081] The composite compositions containing polypeptides and plant extracts of Examples 1-3 and Comparative Examples 1-3 were placed in 10 mL transparent sample bottles and stored at a constant temperature of 20° C. in a dark environment. The changes in ultraviolet absorbance over time after storage for 30 d, 60 d, 120 d, and 180 d were tested, respectively. A 75% ethanol solution was used as a reference solution, and the wavelength range was set to 200-600 nm. The test results are as follows: Figure 1 shown.
[0082] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.
Claims
1. A method for preparing a composite composition containing a polypeptide and a plant extract, characterized in that: The steps include: 1) mixing plant extract, pyrroloquinoline quinone, oligofructose, fermented mulberry juice, fermented polygonatum powder, vitamin B6, vitamin B12, citric acid, pectin and the first stable phase to obtain pre-liquid A; 2) mixing oyster peptide, sea cucumber peptide, earthworm protein peptide and the second stable phase uniformly to prepare pre-liquid B; 3) adding water into the container, heating it to 80-90° C., adding pre-liquid A and pre-liquid B into the water using a microfluidic process, adding hydroxyproline while stirring, and continuing to mix for a period of time after all materials are added, placing it in a cold trap and rapidly cooling it to room temperature, and standing it to obtain a composite composition containing polypeptides and plant extracts; The first stable phase includes carrageenan and aldehyde-modified polysaccharide; The second stable phase includes polar group functionalized chitosan and hydroxytyrosol.
2. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: In the step 1), one or more of the following technical features are also included: 11) The plant extract is obtained by fermenting and extracting Cistanche deserticola, American ginseng, Cornus officinalis, Lycium barbarum, and Eucommia ulmoides male pollen; 12) The fermented polygonatum powder is prepared by fermenting polygonatum, platycodon grandiflorum, green tea and oligofructose.
3. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: The aldehyde-modified polysaccharide is one or more of aldehyde-modified seaweed polysaccharide, aldehyde-modified pullulan polysaccharide and aldehyde-modified pectin polysaccharide.
4. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: The polar group functionalized chitosan is one or more of polyethylene glycol functionalized chitosan, carboxymethyl chitosan, and thiolated chitosan.
5. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: The first stable phase accounts for 2-3% of the total mass of the pre-liquid A.
6. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: The second stable phase accounts for 10-15% of the total mass of the pre-liquid B.
7. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: In the step 3), the pre-fluid A and the pre-fluid B are added into the water by using the microfluidic process: the pre-fluid A is added into the water at a rate of 50-65 mL / min; Add pre-liquid B into the water at a rate of 80-120 mL / min.
8. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 1, characterized in that: In the step 3), the amount of hydroxyproline added is 0.25-0.35% of the mass of the total material.
9. The method for preparing a composite composition containing a polypeptide and a plant extract according to claim 8, characterized in that: In the step 3), the rate of adding hydroxyproline is greater than the rate of adding pre-liquid A, and less than the rate of adding pre-liquid B.
10. A composite composition containing a polypeptide and a plant extract, characterized in that: The method is prepared by any one of claims 1 to 9.