Method for detecting single nucleotide polymorphisms of AQP9 genes in native Chinese cattle

Abstract

The invention discloses a method for detecting single nucleotide polymorphisms of AQP9 genes in native Chinese cattle, which is characterized by taking genomic DNA of the cattle to be detected containing the AQP9 genes as the template and the specific primer pair P6 as the primers and amplifying the exons 6 of the AQP9 genes in the cattle by PCR; and carrying out SSCP detection on the segments of the amplified products and sequencing the products. As the functions of the AQP9 genes relate to such growth traits as weight, height, length, chest circumference and the like, the method screens and detects the molecular genetic markers closely related to the growth traits of the cattle on the DNA level so as to be used for marker-assisted selection (MAS) of the growth traits for the Chinese cattle meat and rapidly build the cattle population with excellent genetic resources.

Description

technical field

[0001] The invention belongs to the field of molecular genetics, relates to using the single nucleotide polymorphism (SNP) of the functional gene of yellow cattle as a molecular genetic marker, in particular to a method for detecting the single nucleotide polymorphism of the AQP9 gene of yellow cattle. Background technique

[0002] In recent years, the beef cattle industry has developed at an ultra-high speed and has become an important industry in the national economy and agriculture of various countries. The development of beef cattle in China still has great potential. In order to gain competitiveness in the international market, meat producers try their best to improve the production performance of meat animals on the premise of ensuring the safety and quality of meat based on the food needs of consumers. This is exactly how to produce the animal product that the consumer welcomes for the breeder, obtains the maximum benefit simultaneously, that is to say...

Examples

Embodiment Construction

[0029] In the present invention, primers are designed to amplify the exon 6 region of the AQP9 gene and the 72bp fragment of the 3'UTR with the conserved sequence of the AQP9 gene, and the genomes of the three breeds of Jiaxian Red Cattle, Luxi Cattle, and Qinchuan Cattle are respectively used as templates for PCR amplification. Then use non-denaturing polyacrylamide gel electrophoresis to detect the single-strand conformation polymorphism of the product. After the polymorphic product is sequenced, find the single nucleotide polymorphism of the amplified fragment; The correlation analysis of traits and its detection method make the nucleotide polymorphism of AQP9 gene a molecular genetic marker that can be quickly and conveniently detected, and provide a basis for accelerating the establishment of cattle populations with high-quality economic traits.

[0030] The following will further describe the present invention in detail, which is an explanation of the present invention ra...

technical field

[0001] The invention belongs to the field of molecular genetics, relates to using the single nucleotide polymorphism (SNP) of the functional gene of yellow cattle as a molecular genetic marker, in particular to a method for detecting the single nucleotide polymorphism of the AQP9 gene of yellow cattle. Background technique

[0002] In recent years, the beef cattle industry has developed at an ultra-high speed and has become an important industry in the national economy and agriculture of various countries. The development of beef cattle in China still has great potential. In order to gain competitiveness in the international market, meat producers try their best to improve the production performance of meat animals on the premise of ensuring the safety and quality of meat based on the food needs of consumers. This is exactly how to produce the animal product that the consumer welcomes for the breeder, obtains the maximum benefit simultaneously, that is to say...

Examples

Embodiment Construction

[0029] In the present invention, primers are designed to amplify the exon 6 region of the AQP9 gene and the 72bp fragment of the 3'UTR with the conserved sequence of the AQP9 gene, and the genomes of the three breeds of Jiaxian Red Cattle, Luxi Cattle, and Qinchuan Cattle are respectively used as templates for PCR amplification. Then use non-denaturing polyacrylamide gel electrophoresis to detect the single-strand conformation polymorphism of the product. After the polymorphic product is sequenced, find the single nucleotide polymorphism of the amplified fragment; The correlation analysis of traits and its detection method make the nucleotide polymorphism of AQP9 gene a molecular genetic marker that can be quickly and conveniently detected, and provide a basis for accelerating the establishment of cattle populations with high-quality economic traits.

[0030] The following will further describe the present invention in detail, which is an explanation of the present invention ra...

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