Sprouted soy peptide crude extract microcapsule powder and preparation method thereof

Abstract

The invention discloses sprouted soy peptide crude extract microcapsule powder and a preparation method thereof. By adopting a biological enzymolysis technique and a complex coacervation spray drying micro-encapsulation technique, taking sprouted soy beans as raw materials and using the hydrolytic action of proteases to process soy proteins of the sprouted soy beans into soy peptides, sprouted soy peptide crude extract is prepared and then is subject to treatment such as complex coacervation, emulsification, homogenization, spray drying and the like. Therefore, the invention provides the preparation method of the sprouted soy peptide crude extract microcapsule powder. The prepared microcapsule powder has high stability and good fluidity and the activity of functional components in the product can be protected.

Description

technical field

[0001] The invention relates to a microcapsule powder in the field of food technology and a preparation method thereof, in particular to a germinated soybean peptide crude extract microcapsule powder and a preparation method thereof. Background technique

[0002] In recent years, some studies have found that after beans are properly germinated, their nutritional components are changed, and a unique flavor and taste can be formed. After soybean seeds germinate, proteins are decomposed into amino acids under the action of their own hydrolytic enzymes, while anti-nutritional factors such as phytic acid and trypsin inhibitors are degraded, and the synthesis of bioactive factors such as soybean isoflavones, vitamin C, and r-aminobutyric acid increases , so that germinated soybeans have richer nutritional value, which is more conducive to human digestion and absorption. However, the traditional cultivation process of bean sprouts often encounters problems such as ...

Examples

Embodiment 1

[0023] 1. Soak soybeans with 0.1g / L chitosan acetic acid aqueous solution containing 50mM glutamic acid (the amount of acetic acid added per liter of solution is 0.1mL) at room temperature for 8h, wash twice with water, and then place at 25°C, 80°C % humidity in the germination box, and take out when the bud length is 30mm as the germinated soybean raw material rich in soybean isoflavones and r-aminobutyric acid.

[0024] 2. Weigh germinated soybeans and put them into a soymilk machine, add 2 times the mass of deionized water to crush and beat, extract the soluble components, filter through a 100-mesh sieve, and freeze-dry the filtrate at -52°C to obtain germinated soybean powder. The germinated soybean powder is subjected to double-enzyme hydrolysis (flavor protease:alkaline protease=7:3 (w / w)). The enzymatic hydrolysis conditions are as follows: germinated soybean powder is preheated in water at 90°C for 10 minutes to dissolve into an emulsion with a mass concentration of 10...

example 2

[0036] Soak soybeans with 0.3g / L chitosan acetic acid aqueous solution containing 50mM glutamic acid (0.3mL acetic acid added per liter solution) at room temperature for 7 hours, wash twice with water, and then place at 25°C, 90% humidity Germinate in a germination box, and take it out when the bud length is 35 mm as a germinated soybean raw material rich in soybean isoflavones and r-aminobutyric acid. Then prepare the crude extract of germinated soybean peptide according to step 2 in Example 1.

[0037] Weigh 12g of the above-mentioned core material (crude extract of germinated soybean peptide), add 300g of deionized water, stir and dissolve at 60°C. Take 0.6mL food-grade Tween-60 and add it to the core material solution, and emulsify with high-speed shear stirring at 12500rpm / min for 5min to form a uniform core material (crude extract of germinated soybean peptide) emulsion. Weigh 31.2g of gum arabic and 4.8g of lactose, add them into the core material (germinated soybean p...

example 3

[0039] Use 0.3g / L chitosan acetic acid aqueous solution containing 50mM glutamic acid (0.3mL acetic acid per liter solution) to soak soybeans at room temperature for 6h, wash twice with water, then place at 25°C, 85% humidity Germinate in a germination box, and take out when the bud length is 30 mm as germinated soybean raw material rich in soybean isoflavones and r-aminobutyric acid. Then prepare the crude extract of germinated soybean peptide according to step 2 described in Example 1.

[0040] Weigh 2 g of the above-mentioned core material (crude extract of germinated soybean peptide), add 50 g of deionized water, stir and dissolve at 55°C. Take 0.25mL of food-grade Tween-60 and add it into the core material (crude extract of germinated soybean peptide) solution, and disperse it with a 300W ultrasonic cell pulverizer for 5 minutes to form a uniform core material emulsion. Weigh 5.6g of gum arabic and 0.4g of lactose, add them into the core material (crude extract of germin...

technical field

[0001] The invention relates to a microcapsule powder in the field of food technology and a preparation method thereof, in particular to a germinated soybean peptide crude extract microcapsule powder and a preparation method thereof. Background technique

[0002] In recent years, some studies have found that after beans are properly germinated, their nutritional components are changed, and a unique flavor and taste can be formed. After soybean seeds germinate, proteins are decomposed into amino acids under the action of their own hydrolytic enzymes, while anti-nutritional factors such as phytic acid and trypsin inhibitors are degraded, and the synthesis of bioactive factors such as soybean isoflavones, vitamin C, and r-aminobutyric acid increases , so that germinated soybeans have richer nutritional value, which is more conducive to human digestion and absorption. However, the traditional cultivation process of bean sprouts often encounters problems such as ...

Examples

Embodiment 1

[0023] 1. Soak soybeans with 0.1g / L chitosan acetic acid aqueous solution containing 50mM glutamic acid (the amount of acetic acid added per liter of solution is 0.1mL) at room temperature for 8h, wash twice with water, and then place at 25°C, 80°C % humidity in the germination box, and take out when the bud length is 30mm as the germinated soybean raw material rich in soybean isoflavones and r-aminobutyric acid.

[0024] 2. Weigh germinated soybeans and put them into a soymilk machine, add 2 times the mass of deionized water to crush and beat, extract the soluble components, filter through a 100-mesh sieve, and freeze-dry the filtrate at -52°C to obtain germinated soybean powder. The germinated soybean powder is subjected to double-enzyme hydrolysis (flavor protease:alkaline protease=7:3 (w / w)). The enzymatic hydrolysis conditions are as follows: germinated soybean powder is preheated in water at 90°C for 10 minutes to dissolve into an emulsion with a mass concentration of 10...

example 2

[0036] Soak soybeans with 0.3g / L chitosan acetic acid aqueous solution containing 50mM glutamic acid (0.3mL acetic acid added per liter solution) at room temperature for 7 hours, wash twice with water, and then place at 25°C, 90% humidity Germinate in a germination box, and take it out when the bud length is 35 mm as a germinated soybean raw material rich in soybean isoflavones and r-aminobutyric acid. Then prepare the crude extract of germinated soybean peptide according to step 2 in Example 1.

[0037] Weigh 12g of the above-mentioned core material (crude extract of germinated soybean peptide), add 300g of deionized water, stir and dissolve at 60°C. Take 0.6mL food-grade Tween-60 and add it to the core material solution, and emulsify with high-speed shear stirring at 12500rpm / min for 5min to form a uniform core material (crude extract of germinated soybean peptide) emulsion. Weigh 31.2g of gum arabic and 4.8g of lactose, add them into the core material (germinated soybean p...

example 3

[0039] Use 0.3g / L chitosan acetic acid aqueous solution containing 50mM glutamic acid (0.3mL acetic acid per liter solution) to soak soybeans at room temperature for 6h, wash twice with water, then place at 25°C, 85% humidity Germinate in a germination box, and take out when the bud length is 30 mm as germinated soybean raw material rich in soybean isoflavones and r-aminobutyric acid. Then prepare the crude extract of germinated soybean peptide according to step 2 described in Example 1.

[0040] Weigh 2 g of the above-mentioned core material (crude extract of germinated soybean peptide), add 50 g of deionized water, stir and dissolve at 55°C. Take 0.25mL of food-grade Tween-60 and add it into the core material (crude extract of germinated soybean peptide) solution, and disperse it with a 300W ultrasonic cell pulverizer for 5 minutes to form a uniform core material emulsion. Weigh 5.6g of gum arabic and 0.4g of lactose, add them into the core material (crude extract of germin...

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