Tissue culture rootage and transplantation methods for wild thymus mongolicus ronn

A technology of tissue culture and thyme, which is applied in the field of transplanting and rooting of wild Mongolian thyme tissue culture, can solve the problem that there is no method for rapid propagation of wild Mongolian thyme, and achieve the effect of accelerated propagation

CN102550403AInactive Publication Date: 2012-07-11北京市园林学校
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2012-07-11
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses tissue culture rootage and transplantation methods for wild thymus mongolicus ronn, which comprises technical links such as rootage culture, exercising seedling, transplanting and the like. Formulas and culture conditions of a culture medium in different culture stages, components and proportions of transplanted matrixes and the like are originally innovated by means of strict multiple comparison test researches, and the survival rate can reach about 80 percent. Due to the invention, a rapid propagation technical system of the wild thymus mongolicus ronn is established to perform artificial propagation, the expanded propagation of the wild thymus mongolicus ronn can be accelerated, and the wild thymus mongolicus ronn can be used as excellent water-saving landscaping ground cover plants.
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Description

technical field

[0001] The invention relates to a tissue culture rapid propagation technology of wild ground cover plants, in particular to a method for tissue culture rooting and transplanting of wild Mongolian thyme. Background technique

[0002] China is rich in wild thyme resources. In China, it is mainly distributed in dry and sunny environments such as rocky mountains and sandy land at an altitude of about 1100-3600m in the "Three Norths" region. Mongolian thyme (Thymus mongolicus Ronn.) is a plant of the genus Thymus in the Labiatae family. It is a perennial dwarf shrub. It is often distributed in the form of a carpet in the wild. The branches, leaves and flowers have a strong fragrance. It has strong drought resistance and can be used as an excellent water-saving landscaping material in urban gardens and green spaces.

[0003] At present, there is no practical method for rapid propagation of wild Mongolian thyme. Contents of the invention

[0004] The purpose of ...

Examples

specific Embodiment

[0019] Rooting culture: Inoculate the seedlings with a height of 1-2cm on the rooting medium to induce rooting. The formula of the medium is: MS+sucrose 30g / L+agar 6g / L+IBA 0.7mg / L, pH5.8-6.0, after 4 days Rooting begins, and the rooting rate reaches 100%.

[0020] Seedling hardening and transplanting: cultivate on the rooting medium for 26-27 days, grow to more than 3cm, remove the sealing film and open the bottle to harden the seedlings for 3-4 days, take out the tissue culture seedlings, take clumps as a unit (5-7 plants per clump) ), wash the culture medium on the root, dip in 0.5% potassium permanganate solution for 5-10s, and transplant to the nutrient soil (peat: perlite: vermiculite=2: 1:1) in a nutrient bowl, maintain a temperature of about 25°C and an air humidity of about 80%, spray water 3-4 times a day, and survive in about 14 days, with a survival rate of about 80%.

[0021] The wild Mongolian thyme (Thymus mongolicus Ronn.) tissue culture rooting and transplant...