Green synthesis method of germanium quantum dot in solution, germanium quantum dot and application thereof in biological imaging

Abstract

The invention provides a green synthesis method of a germanium quantum dot in a solution, a germanium quantum dot and application thereof in biological imaging. The green synthesis method comprises the following step: by taking germanium dioxide as a germanium source, polyvinylpyrrolidone as a protective agent and sodium borohydride as a reducing agent, preparing to obtain the germanium quantum dot. According to the invention, the used raw materials are simple, accessible and stable in property, and basically has no biological toxicity; high temperature and protection of inert gas are not required in the reaction process, thereby ensuring that the green synthesis method is simple and easy to operate; the prepared germanium quantum dot having blue fluorescence has a quantum yield of 26% or above, can be easily separated from the system, has no cytotoxicity and can be well used in biological cell imaging.

Description

technical field

[0001] The invention belongs to the technical field of nanomaterials, and relates to a synthesis method of germanium quantum dots, in particular to a synthesis method of germanium quantum dots in a solution, especially a green synthesis method, germanium quantum dots and their application in biological imaging. Background technique

[0002] Silicon and germanium of the fourth main group, as indirect bandgap semiconductor materials, have been widely used in the field of microelectronics in the past few decades, but due to the extremely low fluorescence quantum yield at room temperature, their applications in the field of optical and optoelectronic devices has always been limited. In recent years, with the rapid development of nanotechnology, new breakthroughs have been made in the research of semiconductor materials silicon and germanium. When they are at the nanometer scale, due to the quantum confinement effect, they will transform into nanomaterials with di...

Examples

Embodiment 1

[0084] Synthesize water-soluble germanium quantum dots as follows:

[0085] (1) Weigh 0.06g of sodium hydroxide and dissolve it in 10ml of water, and ultrasonically dissolve it to make a sodium hydroxide solution;

[0086] (2) Weigh 0.26g germanium dioxide (GeO 2 , purchased from Sigma-Aldrich Shanghai Trading Co., Ltd., product number 483702) was dissolved in the sodium hydroxide solution in step (1), and ultrasonically dissolved to obtain a germanium source solution;

[0087] (3) Dissolving the germanium source in a sodium hydroxide solution under ultrasonic radiation conditions to prepare a germanium source solution, and adjusting the pH value of the germanium source solution in step (2) to between 6 and 7 with dilute hydrochloric acid;

[0088] (4) Take 1ml of the germanium source solution in step (3) and add it to 7ml of water;

[0089] (5) Magnetic stirring at a speed of 1000r / min. Add 0.0250g of polyvinylpyrrolidone (PVP, purchased from Bailingwei Technology, 968832) ...

Embodiment 2

[0112] The germanium quantum dot obtained in embodiment 1 is used for cytotoxicity experiment:

[0113] Lung cancer cells (A549 cell line, purchased from ATCC, number CCL-185) were separated into 10 cells per 35 mm inner diameter of the culture dish. 3 The density of each was inoculated in a petri dish containing 2ml of DMEM medium (purchased from Gibco, brand sh30022.01B, and containing 10vol% fetal bovine serum), at 37°C and 5vol% CO 2 After cultivating at a concentration of 24 h, the culture medium was aspirated, and buffer was added, and the germanium quantum dots The level of apoptosis was detected in the presence of the cells.

[0114] The specific operation steps are as follows:

[0115] (1) Prepare 100 μL of cell suspension in a 64-well plate, and pre-culture the culture plate in an incubator for 24 hours (37°C, CO 2 5vol%);

[0116] (2) Add 10 μL of different concentrations of germanium quantum dots to the culture plate so that the concentrations of germanium quan...

Embodiment 3

[0123] The germanium quantum dot obtained in embodiment 1 is used for cell imaging experiment:

[0124] Lung cancer cells (A549 cell line, purchased from ATCC, number CCL-185) were separated into 10 cells per 35 mm inner diameter of the culture dish. 3 The density of each was inoculated in a petri dish containing 2ml of DMEM medium (purchased from Gibco, brand sh30022.01B) and containing 10% by volume of fetal bovine serum), at 37°C and 5vol% of CO 2 After culturing for 24 hours at the concentration, the medium was sucked out, and 2ml of the medium containing germanium quantum dots (the drug concentration was 2mg / ml) was added to the petri dish, and the petri dish was kept at 37°C and 5vol% CO 2 Incubate for 4 hours at the same concentration, aspirate the medium, wash with phosphate buffer (purchased from Gibco, brand P1020-500) 3 times (5ml each time), and use a fluorescence microscope to image.

[0125] Imaging results such as Figure 6 As shown, it can be seen that after ...

technical field

[0001] The invention belongs to the technical field of nanomaterials, and relates to a synthesis method of germanium quantum dots, in particular to a synthesis method of germanium quantum dots in a solution, especially a green synthesis method, germanium quantum dots and their application in biological imaging. Background technique

[0002] Silicon and germanium of the fourth main group, as indirect bandgap semiconductor materials, have been widely used in the field of microelectronics in the past few decades, but due to the extremely low fluorescence quantum yield at room temperature, their applications in the field of optical and optoelectronic devices has always been limited. In recent years, with the rapid development of nanotechnology, new breakthroughs have been made in the research of semiconductor materials silicon and germanium. When they are at the nanometer scale, due to the quantum confinement effect, they will transform into nanomaterials with di...

Examples

Embodiment 1

[0084] Synthesize water-soluble germanium quantum dots as follows:

[0085] (1) Weigh 0.06g of sodium hydroxide and dissolve it in 10ml of water, and ultrasonically dissolve it to make a sodium hydroxide solution;

[0086] (2) Weigh 0.26g germanium dioxide (GeO 2 , purchased from Sigma-Aldrich Shanghai Trading Co., Ltd., product number 483702) was dissolved in the sodium hydroxide solution in step (1), and ultrasonically dissolved to obtain a germanium source solution;

[0087] (3) Dissolving the germanium source in a sodium hydroxide solution under ultrasonic radiation conditions to prepare a germanium source solution, and adjusting the pH value of the germanium source solution in step (2) to between 6 and 7 with dilute hydrochloric acid;

[0088] (4) Take 1ml of the germanium source solution in step (3) and add it to 7ml of water;

[0089] (5) Magnetic stirring at a speed of 1000r / min. Add 0.0250g of polyvinylpyrrolidone (PVP, purchased from Bailingwei Technology, 968832) ...

Embodiment 2

[0112] The germanium quantum dot obtained in embodiment 1 is used for cytotoxicity experiment:

[0113] Lung cancer cells (A549 cell line, purchased from ATCC, number CCL-185) were separated into 10 cells per 35 mm inner diameter of the culture dish. 3 The density of each was inoculated in a petri dish containing 2ml of DMEM medium (purchased from Gibco, brand sh30022.01B, and containing 10vol% fetal bovine serum), at 37°C and 5vol% CO 2 After cultivating at a concentration of 24 h, the culture medium was aspirated, and buffer was added, and the germanium quantum dots The level of apoptosis was detected in the presence of the cells.

[0114] The specific operation steps are as follows:

[0115] (1) Prepare 100 μL of cell suspension in a 64-well plate, and pre-culture the culture plate in an incubator for 24 hours (37°C, CO 2 5vol%);

[0116] (2) Add 10 μL of different concentrations of germanium quantum dots to the culture plate so that the concentrations of germanium quan...

Embodiment 3

[0123] The germanium quantum dot obtained in embodiment 1 is used for cell imaging experiment:

[0124] Lung cancer cells (A549 cell line, purchased from ATCC, number CCL-185) were separated into 10 cells per 35 mm inner diameter of the culture dish. 3 The density of each was inoculated in a petri dish containing 2ml of DMEM medium (purchased from Gibco, brand sh30022.01B) and containing 10% by volume of fetal bovine serum), at 37°C and 5vol% of CO 2 After culturing for 24 hours at the concentration, the medium was sucked out, and 2ml of the medium containing germanium quantum dots (the drug concentration was 2mg / ml) was added to the petri dish, and the petri dish was kept at 37°C and 5vol% CO 2 Incubate for 4 hours at the same concentration, aspirate the medium, wash with phosphate buffer (purchased from Gibco, brand P1020-500) 3 times (5ml each time), and use a fluorescence microscope to image.

[0125] Imaging results such as Figure 6 As shown, it can be seen that after ...

Images