Bioactive small peptide, synthesis method and use thereof as animal feed additive

Abstract

The invention relates to a bioactive small peptide, a synthesis method and use thereof as an animal feed additive. The bioactive small peptide is linear pentapeptide Tyr-Gly-Gly-Trp-Leu, and is synthetized by adopting a method of gradually combining a joint chain with a segment joint chain, and the obtained peptide is high in purity, can play a role when the adding concentration is very low and has the functions of adjusting animal gastrointestinal flora, promoting the animal digestive function, preventing digestive tract diseases, stimulating the immunologic function of an animal, enhancing the disease resistance of the animal on a plurality of epidemic diseases, greatly reducing the morbidity and the death and culling rate, reducing the production cost, facilitating growth and increasing the weight and is low in production cost.

Description

field of invention

[0001] The invention relates to a bioactive small peptide feed additive, in particular to a bioactive small peptide feed additive and a preparation method thereof. Background technique

[0002] In recent years, in order to one-sidedly pursue profits, some farms (households) have abused feed additives for the purpose of promoting growth, controlling diseases, and increasing lean meat, which not only affects the curative effect but also brings some adverse reactions: such as injection of thymosin preparations, Transfer factor preparations can cause fever and allergic reactions; large doses of lipopolysaccharide can cause immune tolerance; levamisole can cause loss of appetite, nausea, vomiting, abnormal sense of smell, lethargy, fever, allergic reactions, neutropenia, liver function exception etc. The "green technical barriers" of developed countries have seriously restricted my country's animal products from entering the international market. The proportio...

Examples

Embodiment Construction

[0064] The synthetic method of bioactive small peptide feed additive, the steps are as follows:

[0065] 1) Synthesis of Boc-Trp-Leu-OMe (compound 3)

[0066] Slowly add 21mmol of diisopropylethylamine (reagent a: DIEA) dropwise to 30ml of dichloromethane of leucine methyl ester hydrochloride (compound 2, 7mmol) at 0°C, adjust the pH to 7 with DIEA -8, then add Boc-tryptophan (compound 1, 7mmol) and 1-hydroxybenzotriazole 0.92g (HOBt, 7mmol), react at 0°C for 15min, and then add N,N'-di Cyclohexylcarbodiimide 1.54g (DCC, 7.49mmol), reacted at room temperature for 12h (TLC tracking reaction), after stopping the reaction, filtered, evaporated the solvent under reduced pressure, then dissolved in ethyl acetate, followed by citric acid, NaHCO 3 and NaCl solution extraction, combined organic phase, in Na 2 SO 4 Dry for 2 hours, filter, evaporate the solvent under reduced pressure, and purify by 300-400 mesh silica gel column chromatography to obtain 2.74 g of the pure product Bo...

field of invention

[0001] The invention relates to a bioactive small peptide feed additive, in particular to a bioactive small peptide feed additive and a preparation method thereof. Background technique

[0002] In recent years, in order to one-sidedly pursue profits, some farms (households) have abused feed additives for the purpose of promoting growth, controlling diseases, and increasing lean meat, which not only affects the curative effect but also brings some adverse reactions: such as injection of thymosin preparations, Transfer factor preparations can cause fever and allergic reactions; large doses of lipopolysaccharide can cause immune tolerance; levamisole can cause loss of appetite, nausea, vomiting, abnormal sense of smell, lethargy, fever, allergic reactions, neutropenia, liver function exception etc. The "green technical barriers" of developed countries have seriously restricted my country's animal products from entering the international market. The proportio...

Examples

Embodiment Construction

[0064] The synthetic method of bioactive small peptide feed additive, the steps are as follows:

[0065] 1) Synthesis of Boc-Trp-Leu-OMe (compound 3)

[0066] Slowly add 21mmol of diisopropylethylamine (reagent a: DIEA) dropwise to 30ml of dichloromethane of leucine methyl ester hydrochloride (compound 2, 7mmol) at 0°C, adjust the pH to 7 with DIEA -8, then add Boc-tryptophan (compound 1, 7mmol) and 1-hydroxybenzotriazole 0.92g (HOBt, 7mmol), react at 0°C for 15min, and then add N,N'-di Cyclohexylcarbodiimide 1.54g (DCC, 7.49mmol), reacted at room temperature for 12h (TLC tracking reaction), after stopping the reaction, filtered, evaporated the solvent under reduced pressure, then dissolved in ethyl acetate, followed by citric acid, NaHCO 3 and NaCl solution extraction, combined organic phase, in Na 2 SO 4 Dry for 2 hours, filter, evaporate the solvent under reduced pressure, and purify by 300-400 mesh silica gel column chromatography to obtain 2.74 g of the pure product Bo...

Images