Isaria fumosorosea and bacillus thuringiensis wettable powder

Abstract

The invention discloses isaria fumosorosea and bacillus thuringiensis wettable powder. The wettable powder comprises the following ingredients in percentage by mass: 8%-20% of isaria fumosorosea and bacillus thuringiensis, 1-8% of wetting agent, 1-12% of dispersing agent, 0.1-0.5% of ultraviolet protective agent, and the balance of carriers. The weight part ratio of the isaria fumosorosea to bacillus thuringiensis is (10:0.4) to (10:1). The isaria fumosorosea is isaria fumosorosea strain SCAU-IFCF02 which is preserved in CCTCC (China Center for Type Culture Collection) at November 1rst, 2013, with the preservation number of CCTCC NO:M2013526. The bacillus thuringiensis is subsp. Kurstaki. The wettable powder is fast and efficient, has no pesticide residues, postpones the occurrence of pest resistance to insecticide, is environment-friendly, and especially has a good prevention effect on pests on brassicaceous vegetables of plutella xylostella and the like.

Description

technical field

[0001] The invention relates to the field of mixed biological pesticides. More specifically, it relates to a wettable powder of I. fumigatus and Bacillus thuringiensis. Background technique

[0002] The problem of resistance to agricultural pests is one of the hot topics in the world, and it has always been the focus of attention of agricultural science and technology workers. At present, the control of agricultural pests is mainly based on chemical control. The increase and unscientific use of pesticides have led to increasingly serious pest resistance, and more and more types of resistant pests have produced harmful effects on natural enemies. At the same time, the high-intensity use of pesticides will lead to excessive pesticide residues in agricultural products, increase the cost of pesticides for farmers, pollute the environment, and have adverse effects on humans and livestock, which is not conducive to the sustainable development of agriculture. Ther...

Examples

Embodiment 1

[0046] Example 1 The preparation of rose fume-colored I. spp. conidia powder

[0047] In this example, the solid-liquid two-phase fermentation culture is adopted to prepare the conidia powder of the rose fume-colored clavula, and the preparation steps are as follows:

[0048] S1. First-level solid culture, take 50uL of the original bacterial liquid of I. fumigatus strain SCAU-IFCF02 and spread it on the SDAY solid medium (SDAY medium is the most suitable medium), and cultivate it at 24°C, the best culture The time is 15 days;

[0049] S2. Secondary liquid shaking culture, wash the spores on the SDAY solid medium with sterile water containing 0.03% Tween-80, and then filter with double-layer gauze to make 1×10 7 spores / mL suspension, 100uL of the above suspension was inoculated into 200mL Sabouraud's broth (referred to as SDB, the most suitable inoculum), and cultured with shaking at 24°C and 170r / min for 72 hours;

[0050] S3. Tertiary spore-forming culture, low-value old ...

Embodiment 2

[0056] Example 2 Preparation of I. fumigatus and Bacillus thuringiensis wettable powder

[0057] 1. Preparation method:

[0058] S1. Accurately weigh the fume-colored I. spp. conidia powder prepared in Example 1 and the original drug of Bacillus thuringiensis (commercially available);

[0059] S2. Add various additives to the low-temperature airflow mill, control the temperature of the airflow milling below 10°C, and grind it for later use; preferably grind it according to the fineness of 325 mesh,

[0060] S3. Add the mixture of various additives obtained by pulverizing S2 into the carrier according to the proportion, stir evenly, and then add the powder of pink fume-colored I. spp. Mixed wettable powder.

[0061] 2. The wettable powder prepared in this example includes the following components by weight percentage: 9.2% of I. thuringiensis, 0.8% of Bacillus thuringiensis, 3% of OP-10, 2% of Morwet EFW, and 2% of methylene disulfone Sodium Sodium Acid 3%, Morwet D-425 5%...

Embodiment 3

[0064] Example 3 Preparation of wettable powders of I. fumigatus and Bacillus thuringiensis

[0065] 1. The preparation method is the same as in Example 2.

[0066] 2. The wettable powder prepared in this example includes the following components by weight percentage: 11.3% of I. thuringiensis, 0.7% of Bacillus thuringiensis, 4% of AEO-9, 2% of Morwet EFW, fatty alcohol polyoxyethylene ether 2%, Morwet D-425 7%, fluorescein sodium 0.3%, diatomaceous earth 45.9%, talcum powder 26.8%.

[0067] 3. Determination of the performance index of the wettable powder prepared in this embodiment

[0068] The test results show that the product has excellent performance indicators, the germination rate of I. fumigatus spores is ≥85; the germination rate of Bt spores is 82.17%, and the wettability is <1min; the mass suspension rate is 71.9; the suspension rate of spores is 86.7, and the water content 2.96; fineness (through 325 mesh standard sieve) ≥ 98; pH value is 6.5. The performance ...

technical field

[0001] The invention relates to the field of mixed biological pesticides. More specifically, it relates to a wettable powder of I. fumigatus and Bacillus thuringiensis. Background technique

[0002] The problem of resistance to agricultural pests is one of the hot topics in the world, and it has always been the focus of attention of agricultural science and technology workers. At present, the control of agricultural pests is mainly based on chemical control. The increase and unscientific use of pesticides have led to increasingly serious pest resistance, and more and more types of resistant pests have produced harmful effects on natural enemies. At the same time, the high-intensity use of pesticides will lead to excessive pesticide residues in agricultural products, increase the cost of pesticides for farmers, pollute the environment, and have adverse effects on humans and livestock, which is not conducive to the sustainable development of agriculture. Ther...

Examples

Embodiment 1

[0046] Example 1 The preparation of rose fume-colored I. spp. conidia powder

[0047] In this example, the solid-liquid two-phase fermentation culture is adopted to prepare the conidia powder of the rose fume-colored clavula, and the preparation steps are as follows:

[0048] S1. First-level solid culture, take 50uL of the original bacterial liquid of I. fumigatus strain SCAU-IFCF02 and spread it on the SDAY solid medium (SDAY medium is the most suitable medium), and cultivate it at 24°C, the best culture The time is 15 days;

[0049] S2. Secondary liquid shaking culture, wash the spores on the SDAY solid medium with sterile water containing 0.03% Tween-80, and then filter with double-layer gauze to make 1×10 7 spores / mL suspension, 100uL of the above suspension was inoculated into 200mL Sabouraud's broth (referred to as SDB, the most suitable inoculum), and cultured with shaking at 24°C and 170r / min for 72 hours;

[0050] S3. Tertiary spore-forming culture, low-value old ...

Embodiment 2

[0056] Example 2 Preparation of I. fumigatus and Bacillus thuringiensis wettable powder

[0057] 1. Preparation method:

[0058] S1. Accurately weigh the fume-colored I. spp. conidia powder prepared in Example 1 and the original drug of Bacillus thuringiensis (commercially available);

[0059] S2. Add various additives to the low-temperature airflow mill, control the temperature of the airflow milling below 10°C, and grind it for later use; preferably grind it according to the fineness of 325 mesh,

[0060] S3. Add the mixture of various additives obtained by pulverizing S2 into the carrier according to the proportion, stir evenly, and then add the powder of pink fume-colored I. spp. Mixed wettable powder.

[0061] 2. The wettable powder prepared in this example includes the following components by weight percentage: 9.2% of I. thuringiensis, 0.8% of Bacillus thuringiensis, 3% of OP-10, 2% of Morwet EFW, and 2% of methylene disulfone Sodium Sodium Acid 3%, Morwet D-425 5%...

Embodiment 3

[0064] Example 3 Preparation of wettable powders of I. fumigatus and Bacillus thuringiensis

[0065] 1. The preparation method is the same as in Example 2.

[0066] 2. The wettable powder prepared in this example includes the following components by weight percentage: 11.3% of I. thuringiensis, 0.7% of Bacillus thuringiensis, 4% of AEO-9, 2% of Morwet EFW, fatty alcohol polyoxyethylene ether 2%, Morwet D-425 7%, fluorescein sodium 0.3%, diatomaceous earth 45.9%, talcum powder 26.8%.

[0067] 3. Determination of the performance index of the wettable powder prepared in this embodiment

[0068] The test results show that the product has excellent performance indicators, the germination rate of I. fumigatus spores is ≥85; the germination rate of Bt spores is 82.17%, and the wettability is <1min; the mass suspension rate is 71.9; the suspension rate of spores is 86.7, and the water content 2.96; fineness (through 325 mesh standard sieve) ≥ 98; pH value is 6.5. The performance ...

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