Method for depositing bionic hydroxyapatite (HA) on zinc phosphate conversion coating surface

Abstract

The invention relates to a method for depositing bionic hydroxyapatite (HA) on a zinc phosphate conversion coating surface, which comprises the following steps: preparing a simulated body fluid, in which the solute concentrations are as follows: 8.035-16.07g / L sodium chloride, 0.355-0.71g / L sodium bicarbonate, 0.225-0.45g / L potassium chloride, 0.231-0.462g / L dipotassium hydrogen phosphate dihydrate, 0.311-0.622g / L magnesium chloride hexahydrate, 39-58.5mL / L 1mol / L hydrochloric acid, 0.292-0.584g / L calcium chloride and 0.072-0.144g / L sodium sulfate; and cleaning a stainless steel sample of which the surface is provided with the zinc phosphate chemical conversion coating, putting the cleaned sample into the simulated body fluid, putting in a 36-38 DEG C thermostatic water bath oscillator, immersing for 0.5-30 days, taking out, cleaning with deionized water, and drying to obtain the HA coating. The composite coating is beneficial to enhancing the corrosion resistance of the stainless steel implant, has excellent biological activity, and implements early healing and long-term stability of the stainless steel implant.

Description

technical field

[0001] The invention relates to a method for biomimetic deposition of hydroxyapatite on the surface of a zinc phosphate conversion film, belonging to the field of surface modification of biomedical materials. Background technique

[0002] Hydroxyapatite (HA) has attracted much attention because of its excellent biocompatibility, bioactivity, non-toxicity, and strong integration with bone. The preparation of hydroxyapatite by biomimetic deposition has the advantages of simple process, low cost, and the ability to form uniform coatings on irregularly shaped and porous substrates. It has been widely used in many materials. Zinc phosphate (Hopeite, H) is the main crystal phase of zinc phosphate bone cement, and has attracted much attention due to its good biological activity and osteogenic performance. Zinc phosphate conversion coating (HPCC) has the advantages of being insoluble in water, good corrosion resistance, and firmly bonded to the substrate. It is wide...

Examples

Embodiment 1

[0025] (1) prepare zinc phosphate chemical conversion film on stainless steel surface;

[0026] (2) Cleaning: the stainless steel sample with zinc phosphate chemical conversion coating on the surface is cleaned with acetone, absolute ethanol and deionized water respectively for subsequent use;

[0027] (3) Preparation of SBF solution: sodium chloride 12.0525g / L, sodium bicarbonate 0.5325g / L, potassium chloride 0.3375g / L, dipotassium hydrogen phosphate dihydrate 0.3465g / L, magnesium chloride hexahydrate 0.4665g / L, 1mol / L hydrochloric acid 58.5mL / L, calcium chloride 0.438g / L, sodium sulfate 0.108g / L;

[0028] (4) Biomimetic deposition: put the cleaned sample into 200mL simulated body fluid, soak it in a constant temperature water bath oscillator at 37°C for 9 days, take it out, wash it with deionized water, and dry it to obtain the HA film layer.

Embodiment 2

[0030] (1) prepare zinc phosphate chemical conversion film on stainless steel surface;

[0031] (2) Cleaning: the stainless steel sample with zinc phosphate chemical conversion coating on the surface is cleaned with acetone, absolute ethanol and deionized water respectively for subsequent use;

[0032] (3) Preparation of SBF solution: sodium chloride 12.0525g / L, sodium bicarbonate 0.5325g / L, potassium chloride 0.3375g / L, dipotassium hydrogen phosphate dihydrate 0.3465g / L, magnesium chloride hexahydrate 0.4665g / L, 1mol / L hydrochloric acid 58.5mL / L, calcium chloride 0.438g / L, sodium sulfate 0.108g / L;

[0033] (4) Biomimetic deposition: put the cleaned sample into 25 mL of simulated body fluid, soak it in a constant temperature water bath oscillator at 37°C for 15 days, take it out, wash it with deionized water, and dry it to obtain the HA film.

Embodiment 3

[0035] (1) prepare zinc phosphate chemical conversion film on stainless steel surface;

[0036] (2) Cleaning: the stainless steel sample with zinc phosphate chemical conversion coating on the surface is cleaned with acetone, absolute ethanol and deionized water respectively for subsequent use;

[0037] (3) Preparation of SBF solution: sodium chloride 8.035g / L, sodium bicarbonate 0.355g / L, potassium chloride 0.225g / L, dipotassium hydrogen phosphate dihydrate 0.231g / L, magnesium chloride hexahydrate 0.311g / L, 1mol / L hydrochloric acid 39mL / L, calcium chloride 0.292g / L, sodium sulfate 0.072g / L;

[0038] (4) Biomimetic deposition: put the cleaned sample into 25mL simulated body fluid, soak it in a constant temperature water bath oscillator at 37°C for 7 days, take it out, wash it with deionized water, and dry it to obtain the HA film layer.

technical field

[0001] The invention relates to a method for biomimetic deposition of hydroxyapatite on the surface of a zinc phosphate conversion film, belonging to the field of surface modification of biomedical materials. Background technique

[0002] Hydroxyapatite (HA) has attracted much attention because of its excellent biocompatibility, bioactivity, non-toxicity, and strong integration with bone. The preparation of hydroxyapatite by biomimetic deposition has the advantages of simple process, low cost, and the ability to form uniform coatings on irregularly shaped and porous substrates. It has been widely used in many materials. Zinc phosphate (Hopeite, H) is the main crystal phase of zinc phosphate bone cement, and has attracted much attention due to its good biological activity and osteogenic performance. Zinc phosphate conversion coating (HPCC) has the advantages of being insoluble in water, good corrosion resistance, and firmly bonded to the substrate. It is wide...

Examples

Embodiment 1

[0025] (1) prepare zinc phosphate chemical conversion film on stainless steel surface;

[0026] (2) Cleaning: the stainless steel sample with zinc phosphate chemical conversion coating on the surface is cleaned with acetone, absolute ethanol and deionized water respectively for subsequent use;

[0027] (3) Preparation of SBF solution: sodium chloride 12.0525g / L, sodium bicarbonate 0.5325g / L, potassium chloride 0.3375g / L, dipotassium hydrogen phosphate dihydrate 0.3465g / L, magnesium chloride hexahydrate 0.4665g / L, 1mol / L hydrochloric acid 58.5mL / L, calcium chloride 0.438g / L, sodium sulfate 0.108g / L;

[0028] (4) Biomimetic deposition: put the cleaned sample into 200mL simulated body fluid, soak it in a constant temperature water bath oscillator at 37°C for 9 days, take it out, wash it with deionized water, and dry it to obtain the HA film layer.

Embodiment 2

[0030] (1) prepare zinc phosphate chemical conversion film on stainless steel surface;

[0031] (2) Cleaning: the stainless steel sample with zinc phosphate chemical conversion coating on the surface is cleaned with acetone, absolute ethanol and deionized water respectively for subsequent use;

[0032] (3) Preparation of SBF solution: sodium chloride 12.0525g / L, sodium bicarbonate 0.5325g / L, potassium chloride 0.3375g / L, dipotassium hydrogen phosphate dihydrate 0.3465g / L, magnesium chloride hexahydrate 0.4665g / L, 1mol / L hydrochloric acid 58.5mL / L, calcium chloride 0.438g / L, sodium sulfate 0.108g / L;

[0033] (4) Biomimetic deposition: put the cleaned sample into 25 mL of simulated body fluid, soak it in a constant temperature water bath oscillator at 37°C for 15 days, take it out, wash it with deionized water, and dry it to obtain the HA film.

Embodiment 3

[0035] (1) prepare zinc phosphate chemical conversion film on stainless steel surface;

[0036] (2) Cleaning: the stainless steel sample with zinc phosphate chemical conversion coating on the surface is cleaned with acetone, absolute ethanol and deionized water respectively for subsequent use;

[0037] (3) Preparation of SBF solution: sodium chloride 8.035g / L, sodium bicarbonate 0.355g / L, potassium chloride 0.225g / L, dipotassium hydrogen phosphate dihydrate 0.231g / L, magnesium chloride hexahydrate 0.311g / L, 1mol / L hydrochloric acid 39mL / L, calcium chloride 0.292g / L, sodium sulfate 0.072g / L;

[0038] (4) Biomimetic deposition: put the cleaned sample into 25mL simulated body fluid, soak it in a constant temperature water bath oscillator at 37°C for 7 days, take it out, wash it with deionized water, and dry it to obtain the HA film layer.

Images