A kind of porous composite gel-nanofiber oxygen-permeable dressing and preparation method thereof

Abstract

The invention discloses a porous complex gel-nanofiber oxygen permeation dressing and a preparation method thereof. The method comprises the following steps: obtaining a collagen nanofiber membrane by adopting a collagen solution by virtue of an electrostatic spinning method; performing chemical crosslinking on two natural and non-toxic macromolecule polysaccharides such as chitosan and alginic acid, and preparing complex gel; and preparing the porous sponge-nanofiber double-layer composite material in a freeze-drying mode, wherein the upper gel layer of the dressing is in a sponge state during drying and is in a gel state after moisture absorption. The biological wound dressing is prepared under the condition that any toxic chemical crosslinking agent is not added, the process is simple, and the safety is high. The prepared biological wound dressing disclosed by the invention has the characteristics of excellent oxygen permeation performance, water-absorbing quality, water permeability, moisture penetrability and high biocompatibility, a physiological wet environment of the wound surface can be maintained, wound healing and effective haemostasis are promoted, wound infection can be avoided, the recovery is accelerated, the wound surface can be effectively recovered, and the dressing is applied to multiple wound surfaces such as trauma, burns and ulceration.

Description

technical field

[0001] The invention belongs to the technical field of natural polymer materials and biomedical materials, and relates to a medical wound dressing, in particular to a porous composite gel-nano fiber oxygen permeable dressing and a preparation method thereof. Background technique

[0002] Vacuum-assisted closure (VAC) technology, as a new technology for wound treatment, has been widely used in clinical treatment due to its significant advantages of effectively controlling and reducing infection, accelerating wound healing, and reducing the workload of medical staff. Since 1998, the department has started research on the therapeutic mechanism of VAC technology and obtained a number of original research results. We found that the application of VAC therapy will keep the wound environment in a low-oxygen state. Studies have shown that although in the early process of wound healing, Hypoxic environment is conducive to wound capillary formation, but as wound healin...

Examples

Embodiment 1

[0037] (1) Collagen is placed in the acetic acid solution that mass fraction is 1mol / L, is mixed with the collagen solution that mass concentration is 8% (w / w);

[0038] (2) Pour the collagen solution obtained in step (1) into a 10mL syringe, control the flow rate to 1mL / h, the electrospinning voltage is 30kV, the receiving distance is 15cm, and the electrospinning time is 24h. Collagen nanofibers were prepared by electrospinning, and the temperature and relative humidity of electrospinning were 25°C and 45% respectively;

[0039] (3) Place the nanofibrous membrane prepared in step (2) in a vacuum drying oven, and dry at 30°C for 6 hours to remove residual solvent; then cross-link in glutaraldehyde vapor, the volume concentration of glutaraldehyde 1%, the cross-linking time is 6h;

[0040] (4) dissolving chitosan in a mass concentration of 2% glacial acetic acid aqueous solution to prepare a mass concentration of 2% chitosan solution, the prepared 1mg / mL calcium chloride aque...

Embodiment 2

[0044] (1) Collagen is placed in the acetic acid solution that mass fraction is 1mol / L, is mixed with the collagen solution that mass concentration is 8% (w / w);

[0045] (2) Pour the collagen solution obtained in step (1) into a 10mL syringe, control the flow rate to 1mL / h, the electrospinning voltage is 30kV, the receiving distance is 15cm, and the electrospinning time is 48h. Collagen nanofibers were prepared by electrospinning, and the temperature and relative humidity of electrospinning were 25°C and 45% respectively;

[0046] (3) Place the nanofibrous membrane prepared in step (2) in a vacuum drying oven, and dry at 30°C for 12 hours to remove residual solvent; then cross-link in glutaraldehyde vapor, the volume concentration of glutaraldehyde is 2%, and the cross-linking time is 12h;

[0047] (4) dissolving chitosan in a mass concentration of 2% glacial acetic acid aqueous solution to prepare a mass concentration of 2% chitosan solution, the prepared 1mg / mL calcium chlo...

Embodiment 3

[0051] (1) Collagen is placed in the acetic acid solution that mass fraction is 1mol / L, is mixed with the collagen solution that mass concentration is 8% (w / w);

[0052] (2) Pour the collagen solution obtained in step (1) into a 10mL syringe, control the flow rate to 1mL / h, the electrospinning voltage is 30kV, the receiving distance is 15cm, and the electrospinning time is 24h. Collagen nanofibers were prepared by electrospinning, and the temperature and relative humidity of electrospinning were 25°C and 45% respectively;

[0053] (3) Place the nanofibrous membrane prepared in step (2) in a vacuum drying oven, and dry at 30°C for 6 hours to remove residual solvent; then cross-link in glutaraldehyde vapor, the volume concentration of glutaraldehyde 1%, the cross-linking time is 6h;

[0054] (4) dissolving chitosan in a mass concentration of 2% glacial acetic acid aqueous solution to prepare a mass concentration of 2% chitosan solution, the prepared 1mg / mL calcium chloride aque...

technical field

[0001] The invention belongs to the technical field of natural polymer materials and biomedical materials, and relates to a medical wound dressing, in particular to a porous composite gel-nano fiber oxygen permeable dressing and a preparation method thereof. Background technique

[0002] Vacuum-assisted closure (VAC) technology, as a new technology for wound treatment, has been widely used in clinical treatment due to its significant advantages of effectively controlling and reducing infection, accelerating wound healing, and reducing the workload of medical staff. Since 1998, the department has started research on the therapeutic mechanism of VAC technology and obtained a number of original research results. We found that the application of VAC therapy will keep the wound environment in a low-oxygen state. Studies have shown that although in the early process of wound healing, Hypoxic environment is conducive to wound capillary formation, but as wound healin...

Examples

Embodiment 1

[0037] (1) Collagen is placed in the acetic acid solution that mass fraction is 1mol / L, is mixed with the collagen solution that mass concentration is 8% (w / w);

[0038] (2) Pour the collagen solution obtained in step (1) into a 10mL syringe, control the flow rate to 1mL / h, the electrospinning voltage is 30kV, the receiving distance is 15cm, and the electrospinning time is 24h. Collagen nanofibers were prepared by electrospinning, and the temperature and relative humidity of electrospinning were 25°C and 45% respectively;

[0039] (3) Place the nanofibrous membrane prepared in step (2) in a vacuum drying oven, and dry at 30°C for 6 hours to remove residual solvent; then cross-link in glutaraldehyde vapor, the volume concentration of glutaraldehyde 1%, the cross-linking time is 6h;

[0040] (4) dissolving chitosan in a mass concentration of 2% glacial acetic acid aqueous solution to prepare a mass concentration of 2% chitosan solution, the prepared 1mg / mL calcium chloride aque...

Embodiment 2

[0044] (1) Collagen is placed in the acetic acid solution that mass fraction is 1mol / L, is mixed with the collagen solution that mass concentration is 8% (w / w);

[0045] (2) Pour the collagen solution obtained in step (1) into a 10mL syringe, control the flow rate to 1mL / h, the electrospinning voltage is 30kV, the receiving distance is 15cm, and the electrospinning time is 48h. Collagen nanofibers were prepared by electrospinning, and the temperature and relative humidity of electrospinning were 25°C and 45% respectively;

[0046] (3) Place the nanofibrous membrane prepared in step (2) in a vacuum drying oven, and dry at 30°C for 12 hours to remove residual solvent; then cross-link in glutaraldehyde vapor, the volume concentration of glutaraldehyde is 2%, and the cross-linking time is 12h;

[0047] (4) dissolving chitosan in a mass concentration of 2% glacial acetic acid aqueous solution to prepare a mass concentration of 2% chitosan solution, the prepared 1mg / mL calcium chlo...

Embodiment 3

[0051] (1) Collagen is placed in the acetic acid solution that mass fraction is 1mol / L, is mixed with the collagen solution that mass concentration is 8% (w / w);

[0052] (2) Pour the collagen solution obtained in step (1) into a 10mL syringe, control the flow rate to 1mL / h, the electrospinning voltage is 30kV, the receiving distance is 15cm, and the electrospinning time is 24h. Collagen nanofibers were prepared by electrospinning, and the temperature and relative humidity of electrospinning were 25°C and 45% respectively;

[0053] (3) Place the nanofibrous membrane prepared in step (2) in a vacuum drying oven, and dry at 30°C for 6 hours to remove residual solvent; then cross-link in glutaraldehyde vapor, the volume concentration of glutaraldehyde 1%, the cross-linking time is 6h;

[0054] (4) dissolving chitosan in a mass concentration of 2% glacial acetic acid aqueous solution to prepare a mass concentration of 2% chitosan solution, the prepared 1mg / mL calcium chloride aque...

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