Artificial breeding method for spinibarbus sinensis

Abstract

The invention relates to an artificial breeding method for spinibarbus sinensis. The method includes the following steps of intensive culture of broodstock, artificial spawning and artificial insemination; the artificial spawning adopts a two-needle spawning method, wherein when the first needle injection is carried out, only luteinizing hormone is used for releasing hormone LRH-A2, the dose of the first needle injection for female fishes is 1.5-2.5 micrograms / kg, and the dose of the first needle injection for male fishes is 0 or halved compared to that for the female fishes; the second needle injection is carried out in 10-14h after the first needle injection, and the dose of the second needle injection for the male fishes is halved compared to that for the female fishes; the artificial insemination is carried out by putting parents capable of meeting the gonadal maturity requirement into a container with a small amount of water to make cloacal apertures of the female and male fishes opposite and press abdomens of the female and male fishes to make sperms and eggs flow out and combine for fertilization. Through the artificial breeding method, the average brood amount per gram weight of the female fishes on which the intensive culture of the parents is carried out reaches about 26 grains, the spawning rate of parents reaches 90.91-94.44%, and the fertilization rate reaches 92-96%.

Description

technical field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for artificial propagation of Chinese barb. Background technique

[0002] Chinese barbed barb (Spinibarbus sinensis), commonly known as Qingbo, Wulin, Qingban. Belonging to Cyprinidae, Cyprinidae, Carp subfamily, and Barb genus, it is mainly distributed in the upper reaches of the Yangtze River and its tributaries in my country. It is one of the important and valuable economic fish in the Jialing River. There are "one bream, two rocks (Iwahara carp), Sanqingbo (Chinese Barbed Barb)". Chinese barbed barb grows fast, has a large body, strong disease resistance, rich nutrition, and delicious meat. It is a famous and precious fish with Chinese characteristics. Chinese barbed barb has been artificially propagated in Sichuan and Hubei, mostly using PG, LRH-A 2 , HCG and DOM and other hormone compatibility for artificial induced labor, fertilization. Because ...

Examples

Embodiment 1

[0039] Source of parents: In October 2010, Wuwei County Aquaculture Farm in Anhui purchased about 5cm barb fry from Hubei Province Jingzhou Chengguan Breeding Co., Ltd. and cultivated them until some of them reached sexual maturity in 2015. In 2015, in order not to damage the broodstock, no development Artificial breeding, continued to breed until 2016.

[0040] At the same time, in October 2015, step S1, parental strengthening cultivation: select Chinese barbs with strong physique and no injuries as broodstock and put them into the breeding pond for cultivation. The area of ​​the breeding pond is 600m 2 , pond depth 2m, depth of water in the pond 1m, aerators are installed in the cultivation pond, the number of broodstock in the cultivation pond is 900kg, wherein the body weight of male fish is 0.73kg, and the body weight of female fish is 0.94kg; Feed crude protein in the grass carp pellet feed of 28wt% to intensify cultivation; By March 2016, start feeding ryegrass 100kg at...

Embodiment 2

[0047] This embodiment is basically the same as the method in embodiment 1, and the parameters are adjusted and changed:

[0048] In the step S1, the cultivation pond area is 667m 2 , deep 2.5m in the pond, 1.5m deep in the pond, the aerator is an impeller type aerator, and the number of broodstock in the cultivation pond is 1000kg, wherein the body weight of the male fish is 0.8kg, and the body weight of the female fish is 1.3kg; After going down to the pond, the crude protein of grass carp pellet feed is 30wt%; 125kg of ryegrass is fed in March; 300kg of duckweed is fed every day until April; 75kg of ryegrass is fed every day until May; Once every 4 weeks; flushing time 2.5h.

[0049] In the step S2, the weight of the female fish is 1.5 kg, and the weight of the male fish is 1.0 kg; the dose of the first injection of the female fish is 2 μg / kg, and the first injection of the male fish is not performed; the second injection is performed 14 hours after the first injection , ...

Embodiment 3

[0053] This embodiment is basically the same as Embodiment 1, and the parameters that are adjusted and changed are:

[0054] In the step S1, the area of ​​the cultivation pond is 700m 2 , Pond deep 3m, water depth 2m in the pond, described aerator is impeller type aerator, and the quantity that breeds parent fish in a suitable place to breed in the cultivation pond is 1100kg, and wherein male fish body weight is at 0.95kg, and female fish body weight is at 1.42kg; Parent fish goes down pond The crude protein of grass carp pellet feed after feeding is 35wt%; 150kg of ryegrass is fed in March; 350kg of duckweed is fed every day until April; 100kg of ryegrass is fed every day until May; the water is changed for 4 weeks Once; flushing time 2h.

[0055] In the step S2, the weight of the female fish is 1.9 kg, and the weight of the male fish is 1.1 kg; the dose of the first female fish injection is 2.5 μg / kg; the second injection is performed 12 hours after the first injection, and...

technical field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for artificial propagation of Chinese barb. Background technique

[0002] Chinese barbed barb (Spinibarbus sinensis), commonly known as Qingbo, Wulin, Qingban. Belonging to Cyprinidae, Cyprinidae, Carp subfamily, and Barb genus, it is mainly distributed in the upper reaches of the Yangtze River and its tributaries in my country. It is one of the important and valuable economic fish in the Jialing River. There are "one bream, two rocks (Iwahara carp), Sanqingbo (Chinese Barbed Barb)". Chinese barbed barb grows fast, has a large body, strong disease resistance, rich nutrition, and delicious meat. It is a famous and precious fish with Chinese characteristics. Chinese barbed barb has been artificially propagated in Sichuan and Hubei, mostly using PG, LRH-A 2 , HCG and DOM and other hormone compatibility for artificial induced labor, fertilization. Because ...

Examples

Embodiment 1

[0039] Source of parents: In October 2010, Wuwei County Aquaculture Farm in Anhui purchased about 5cm barb fry from Hubei Province Jingzhou Chengguan Breeding Co., Ltd. and cultivated them until some of them reached sexual maturity in 2015. In 2015, in order not to damage the broodstock, no development Artificial breeding, continued to breed until 2016.

[0040] At the same time, in October 2015, step S1, parental strengthening cultivation: select Chinese barbs with strong physique and no injuries as broodstock and put them into the breeding pond for cultivation. The area of ​​the breeding pond is 600m 2 , pond depth 2m, depth of water in the pond 1m, aerators are installed in the cultivation pond, the number of broodstock in the cultivation pond is 900kg, wherein the body weight of male fish is 0.73kg, and the body weight of female fish is 0.94kg; Feed crude protein in the grass carp pellet feed of 28wt% to intensify cultivation; By March 2016, start feeding ryegrass 100kg at...

Embodiment 2

[0047] This embodiment is basically the same as the method in embodiment 1, and the parameters are adjusted and changed:

[0048] In the step S1, the cultivation pond area is 667m 2 , deep 2.5m in the pond, 1.5m deep in the pond, the aerator is an impeller type aerator, and the number of broodstock in the cultivation pond is 1000kg, wherein the body weight of the male fish is 0.8kg, and the body weight of the female fish is 1.3kg; After going down to the pond, the crude protein of grass carp pellet feed is 30wt%; 125kg of ryegrass is fed in March; 300kg of duckweed is fed every day until April; 75kg of ryegrass is fed every day until May; Once every 4 weeks; flushing time 2.5h.

[0049] In the step S2, the weight of the female fish is 1.5 kg, and the weight of the male fish is 1.0 kg; the dose of the first injection of the female fish is 2 μg / kg, and the first injection of the male fish is not performed; the second injection is performed 14 hours after the first injection , ...

Embodiment 3

[0053] This embodiment is basically the same as Embodiment 1, and the parameters that are adjusted and changed are:

[0054] In the step S1, the area of ​​the cultivation pond is 700m 2 , Pond deep 3m, water depth 2m in the pond, described aerator is impeller type aerator, and the quantity that breeds parent fish in a suitable place to breed in the cultivation pond is 1100kg, and wherein male fish body weight is at 0.95kg, and female fish body weight is at 1.42kg; Parent fish goes down pond The crude protein of grass carp pellet feed after feeding is 35wt%; 150kg of ryegrass is fed in March; 350kg of duckweed is fed every day until April; 100kg of ryegrass is fed every day until May; the water is changed for 4 weeks Once; flushing time 2h.

[0055] In the step S2, the weight of the female fish is 1.9 kg, and the weight of the male fish is 1.1 kg; the dose of the first female fish injection is 2.5 μg / kg; the second injection is performed 12 hours after the first injection, and...

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