Culturing technology for upright-ladybell-root in-vitro regeneration tissue

Abstract

The invention discloses a culturing technology for upright-ladybell-root in-vitro regeneration tissue. Upright ladybell roots are campanulaceae plants, and medicinal materials of the upright ladybellroots are dried roots of adenophora tetraphylla or adenophora hunanensis. Digging is carried out in spring and autumn, stem leaves and fibrous roots are removed, soil is cleaned, cork is scraped, anddrying in the sun is carried out. The upright ladybell roots are long conical or long cylindrical, some roots are slightly curved or warped, and a plurality of half-moon-shaped stem marks exist on theperiphery, and are in disc section shapes; surfaces are yellowish white or light brownish yellow, residual brown cork frequently exists at sunken portions, a plurality of deep-sunken transverse striations are arranged at the upper portions and are intermittent annular striations, and longitudinal wrinkles and longitudinal furrows are arranged at the lower portions. The weight is light and porous,bending easily occurs, and fracture surfaces are yellowish white and uneven, and have multiple fractures. The smell is feeble, and the flavor is sweet and light; the roots which are rough and long instrip, dry, rich, yellowish white in color and completely-clean in scarfskin are good. According to the culturing technology, the upright ladybell roots with bud stem sections serve as explants, upright-ladybell-root in-vitro regeneration is achieved through processes such as bud induction, bud enrichment culturing, rooting culturing, seedling hardening and transplanting, and a technical supportis provided for mass breeding, rapid propagation and new-species popularization.

Description

technical field

[0001] The invention relates to plant tissue culture, in particular to a technology for culture of isolated regenerated tissue of Nansha ginseng. Background technique

[0002] Nanshashen is the dried root of Campanulaceae plants, Adenophora verticillum or Adenophora apricot. Excavated in spring and autumn, remove the stems, leaves and fibrous roots, wash the soil, scrape off the cork, and dry in the sun. Properties of medicinal material: The roots are long conical or long cylindrical, some are slightly curved or twisted, and there are many half-moon-shaped stem scars around them, which are disc-shaped. The surface is yellowish-white or light brownish-yellow, and there are often residual tan cork in the depressions. The upper part often has deep horizontal lines, which are intermittent ring lines, and the lower part has longitudinal wrinkles and longitudinal grooves. The body is light and vesicular, easy to break, the cross-section is yellowish white, uneven...

Examples

Embodiment 1

[0015] (1) Bud induction culture: use the isolated regenerated bud stem section of Nansha Radix as explants, cut off the leaves, cut into 4.8 long sections with 4 axillary buds, soak them in washing powder solution for 4 minutes, and then wash them with soft water. Gently scrub the surface of the explant with a brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 4 seconds, then wash it twice with sterile water, and then use Disinfect with 0.2% mercuric chloride solution for 9 minutes, rinse with sterile water for 3 times, then dry the water drops on the surface with sterile filter paper, then inoculate into bud induction medium for bud induction, and culture in total darkness at 27°C for 2 days after inoculation , then 9 hours of light every day, and the light intensity was cultivated for 29 days under the condition of 1400lx, and the bud induction...

Embodiment 2

[0020] (1) Bud induction culture: Use the stem section of Nansha ginseng with buds as explants, cut off the leaves, cut into 8.0 long segments with 6 axillary buds, soak them in washing powder solution for 13 minutes, and then gently brush them with a soft brush. Lightly scrub the surface of the explant to remove the dust and part of the bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 18 seconds, then wash it with sterile water for 8 times, and then use 0.1% L Mercury solution was sterilized for 18 minutes, rinsed with sterile water for 7 times, and then wiped dry the water droplets on the surface with sterile filter paper, and then inoculated into bud induction medium for bud induction. Illuminated for 13 hours, and the light intensity was cultivated for 33 days under the condition of 1800lx, and the bud induction rate and growth situation were counted, and the bud induction r...

technical field

[0001] The invention relates to plant tissue culture, in particular to a technology for culture of isolated regenerated tissue of Nansha ginseng. Background technique

[0002] Nanshashen is the dried root of Campanulaceae plants, Adenophora verticillum or Adenophora apricot. Excavated in spring and autumn, remove the stems, leaves and fibrous roots, wash the soil, scrape off the cork, and dry in the sun. Properties of medicinal material: The roots are long conical or long cylindrical, some are slightly curved or twisted, and there are many half-moon-shaped stem scars around them, which are disc-shaped. The surface is yellowish-white or light brownish-yellow, and there are often residual tan cork in the depressions. The upper part often has deep horizontal lines, which are intermittent ring lines, and the lower part has longitudinal wrinkles and longitudinal grooves. The body is light and vesicular, easy to break, the cross-section is yellowish white, uneven...

Examples

Embodiment 1

[0015] (1) Bud induction culture: use the isolated regenerated bud stem section of Nansha Radix as explants, cut off the leaves, cut into 4.8 long sections with 4 axillary buds, soak them in washing powder solution for 4 minutes, and then wash them with soft water. Gently scrub the surface of the explant with a brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 4 seconds, then wash it twice with sterile water, and then use Disinfect with 0.2% mercuric chloride solution for 9 minutes, rinse with sterile water for 3 times, then dry the water drops on the surface with sterile filter paper, then inoculate into bud induction medium for bud induction, and culture in total darkness at 27°C for 2 days after inoculation , then 9 hours of light every day, and the light intensity was cultivated for 29 days under the condition of 1400lx, and the bud induction...

Embodiment 2

[0020] (1) Bud induction culture: Use the stem section of Nansha ginseng with buds as explants, cut off the leaves, cut into 8.0 long segments with 6 axillary buds, soak them in washing powder solution for 13 minutes, and then gently brush them with a soft brush. Lightly scrub the surface of the explant to remove the dust and part of the bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 18 seconds, then wash it with sterile water for 8 times, and then use 0.1% L Mercury solution was sterilized for 18 minutes, rinsed with sterile water for 7 times, and then wiped dry the water droplets on the surface with sterile filter paper, and then inoculated into bud induction medium for bud induction. Illuminated for 13 hours, and the light intensity was cultivated for 33 days under the condition of 1800lx, and the bud induction rate and growth situation were counted, and the bud induction r...