Method for simultaneously selecting and breeding pepper male sterile line and homozygous restorer gene line by using recovery gene linkage marker

A male sterile line and gene restoration technology, applied in the field of breeding, to achieve the effect of reducing the number of self-breeding generations, reducing the impact of environmental factors, and being easy to obtain

CN110129481AActive Publication Date: 2019-08-16QINGDAO AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2019-08-16

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Abstract

The invention provides a method for simultaneously selecting and breeding a pepper male sterile line and a homozygous restorer gene line by using a recovery gene linkage marker. The present inventionprovides a method for identifying a single plant having a homozygous restorer gene by constructing a male sterile pool and a fertile pond in a F2 generation population. By using the Capsicum-R-82CAPSmarker of the present invention, the method can distinguish whether the pepper has the homozygous restorer gene line, and also can identify the cytoplasmic male sterile plant at the DNA level, which can reduce the breeding workload increased due to infertility caused by environmental factors. The original pepper breeding material required by the invention is easy to obtain, the method can reduce the breeding generation, shortens the breeding time, and quickly enriches the breeder's pepper male sterile three-line material storage.
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Description

technical field

[0001] The invention belongs to the technical field of breeding, and in particular relates to a method for simultaneously breeding capsicum male sterile lines and homozygous restorer gene lines by utilizing a restorer gene linkage marker. Background technique

[0002] Capsicum belongs to the Solanaceae capsicum plant, because it is rich in capsanthin, capsaicin and other substances, it is very popular among people. At present, the demand for capsanthin is increasing, and it is widely used in feed additives, cosmetics, and pharmaceutical industries. Pigment pepper is the main raw material for extracting capsanthin, and has been widely promoted in Xinjiang, Gansu, Inner Mongolia and other places. However, in terms of production, there are few varieties of peppers with special pigments, and the quality is uneven. Therefore, the breeding of excellent first-generation hybrids with high capsanthin content is very important for the development of the pepper industr...

Examples

Embodiment 1

[0045]The invention uses the capsicum male sterile three-line hybrid as the basic material, and constructs the F2 generation segregation population in a 40-mesh isolation net room. According to the results of the field fertility survey at the flowering stage, the sterile pool and the fertile pool were constructed, and the polymorphism screening was carried out using the pepper restorer gene linkage marker. As a result of electrophoresis in the fertile pool, when the band type is two bands at a molecular weight of 82bp, one molecular weight is different from the size in the sterile pool, and the other is the same, the restoration gene linkage marker can be used in the F2 population The identification of homozygous individual plants at the restorer gene locus was carried out. Then, all fertile plants in the F2 population were screened by molecular markers, and the plants with only one band and different molecular weight from those in the sterile pool were selected, that is, the ...