A nucleic acid extraction device

Abstract

The present invention belongs to the technical field of nucleic acid extraction, and provides a nucleic acid extraction device. A nucleic acid extraction device of the present invention comprises: a double-chamber component, wherein the double-chamber component comprises an extraction cavity tube and an absorption cavity tube; wherein the absorption cavity tube and the extraction cavity tube are detachably connected and communicated; one end of the extraction cavity tube is open and is used to extract nucleic acid; and an absorption component, wherein the absorption component is installed in the double-chamber component, wherein the absorption component comprises an adjustment piston and a push rod, wherein the adjustment piston is installed on the push rod, and the adjustment piston reciprocates in the double-chamber component through the push rod; the adjustment piston can change the diameter; wherein when the nucleic acid mixed solution enters the double-chamber component, the push rod is driven to reciprocate, so that the adjustment piston reciprocates up and down. In summary, the device is not only reasonably designed, but also simple to operate, and can effectively achieve separation, purification and preservation, and effectively solves the adverse effects brought by the above-mentioned impurities, so the device is suitable for industry promotion.

Description

Technical Field

[0001] The invention relates to the technical field of nucleic acid extraction, and in particular to a nucleic acid extraction device. Background Art

[0002] During the initial extraction of nucleic acid, nucleic acid and other impurities are often extracted together, and then separated to obtain pure nucleic acid; however, in this process, due to human factors, the waiting time is uncertain; this will cause the above-mentioned nucleic acid to be affected by impurities;

[0003] Therefore, we propose an extraction device that can effectively reduce the impact of impurities during the initial extraction of nucleic acids, thereby effectively solving the above problems. Summary of the invention

[0004] In view of the defects in the prior art, the present invention provides a nucleic acid extraction device to effectively reduce the influence of impurities.

[0005] In the first aspect, the present invention provides a nucleic acid extraction device, comprising: a double-chamber component, the double-chamber component comprising an extraction chamber and an absorption chamber; wherein the absorption chamber is detachably connected to the extraction chamber and communicates with each other; one end of the extraction chamber is open and is used to extract nucleic acid; and an absorption component, the absorption component is installed in the double-chamber component, the absorption component comprises an adjustment piston and a push rod, the adjustment piston is installed on the push rod, and the adjustment piston reciprocates in the double-chamber component through the push rod; the adjustment piston can change its diameter; wherein when the nucleic acid mixed solution enters the double-chamber component, the push rod is driven to reciprocate, so that the adjustment piston reciprocates up and down.

[0006] Furthermore, the extraction cavity tube also has an installation slot, which is arranged on the side wall of the extraction cavity tube; the suction cavity tube also has a fixed block, which is arranged on the side wall of the suction cavity tube; wherein the fixed block is detachably connected to the installation slot. In actual use, this design effectively realizes the effective connection and installation and fixation of the two components in the double cavity part, namely the extraction cavity tube and the suction cavity tube.

[0007] Furthermore, the extraction chamber tube also has an extraction chamber and a side wall hole, the side wall hole is located on the side wall of the extraction chamber and penetrates the mounting slot; the suction chamber tube also has a suction chamber and a connecting hole, the connecting hole is located on the side wall of the suction chamber and penetrates the fixed block; wherein the connecting hole is in communication with the side wall hole. In actual use, the purpose of this design is to facilitate the mixed solution of nucleic acids, that is, the mixed liquid in the process of nucleic acid extraction, to enter the suction chamber tube from the extraction chamber tube, so that separation can be achieved and the purity of nucleic acids can be improved.

[0008] Furthermore, it also includes: an extraction needle tube, one end of the extraction cavity tube is provided with an extraction port, and the extraction port is provided at the extraction chamber and communicates with the extraction chamber; the extraction needle tube can be detachably installed at the extraction port. In actual use, the purpose of this design is to facilitate extraction, and the accessories are available and can be used directly, reducing the design cost.

[0009] Furthermore, a closing cap is also included, which is detachably mounted on the extraction needle tube and is used to close the extraction chamber. In actual use, the purpose of this design is to close the extraction chamber, facilitate pressure changes, and make subsequent separation work more thorough.

[0010] Furthermore, the suction piece is provided at two locations, and is installed at the extraction cavity and the suction cavity respectively. In actual use, this design effectively realizes the ability to perform reciprocating pressing at the extraction cavity and the suction cavity respectively, which can accelerate the separation effect and improve the purity of nucleic acid.

[0011] Furthermore, the adjustment piston includes a sealing abutment rubber sleeve and a variable diameter block; the sealing abutment rubber sleeve is installed on the outer surface of the variable diameter block, and the variable diameter block is provided with at least one expansion section, wherein the expansion section is provided at one end of the variable diameter block, and a plurality of expansion sections are annular and evenly distributed along the center of the variable diameter block. In actual use, the purpose of this design is to achieve diameter change and adjust the diameter, thereby adjusting the gap between the sealing abutment rubber sleeve and the inner wall of the extraction chamber and the suction chamber during the contact process. In this way, the adjustment can be ensured. Through the expandable expansion section, the expansion section is annularly arranged, and the diameter change of the variable diameter block can be achieved by opening and closing the expansion section, thereby achieving adjustment.

[0012] Furthermore, the adjustment piston also includes an abutment cone head and an adjustment screw, wherein the abutment cone head is conical, one end of the abutment cone head is connected to one end of the adjustment screw, and the other end of the abutment cone head reciprocates in the variable diameter block, and the outer wall of the abutment cone head abuts against the expansion section. In actual use, the purpose of this design is to realize the expansion section movement of the above-mentioned variable diameter block, so that multiple expansion sections can be expanded outward after being abutted by force, and the overall opening and closing effect can be achieved, which is essentially similar to opening an umbrella; in this way, the distance between the sealing abutment rubber sleeve outside the expansion section and the inner wall of the extraction cavity or the suction cavity is changed, thereby adjusting the pressure, which is convenient for realizing the oscillation of the nucleic acid mixture, thereby realizing the stratification of the nucleic acid mixture, and facilitating the subsequent extraction of nucleic acids.

[0013] Furthermore, the push rod also has a threaded hole, and the threaded hole is arranged along the axial direction of the push rod and passes through the push rod; the adjustment screw is rotatably installed in the threaded hole, and the other end of the adjustment screw extends outward.

[0014] Furthermore, the adjusting screw is provided with an adjusting nut, and the adjusting nut is arranged at the other end of the adjusting screw and is used to rotate the adjusting screw.

[0015] It can be seen from the above technical solution that the beneficial effects of the nucleic acid extraction device provided by the present invention are:

[0016] (1) In actual work, the double-chamber structure design adopted in this design can achieve convenient separation and further purification effect;

[0017] (2) The suction part adopts a variable diameter adjustment piston, so that the pressure in the extraction cavity and the suction cavity in the double-chamber part can be effectively changed, which not only realizes the pressure difference, but also facilitates the extraction into the suction cavity after stratification and facilitates the withdrawal of the adjustment piston there, thereby achieving early extraction.

[0018] (3) At the same time, the dual-chamber structural design and the use of the adjustment piston can effectively achieve a vacuum environment, thereby preserving the extracted nucleic acid solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the specific embodiments of the present invention, the following briefly introduces the drawings required for the specific embodiments or the prior art description. In all the drawings, each element or part is not necessarily drawn according to the actual scale.

[0020] Figure 1 A schematic diagram of the main structure of a nucleic acid extraction device provided by an embodiment of the present invention;

[0021] Figure 2 A schematic structural diagram of a sealing abutment rubber sleeve installed on a diameter-changing block in a nucleic acid extraction device provided by an embodiment of the present invention;

[0022] Figure 3 for Figure 2 Schematic diagram of AA structure shown;

[0023] Reference numerals:

[0024] Double-chamber component 1, extraction chamber 11, mounting slot 111, extraction chamber 112, side wall hole 113, extraction port 114, fixed block 121, suction chamber 122, connecting hole 123, suction chamber 12, suction component 2, adjustment piston 21, sealing abutment rubber sleeve 211, reducing block 212, expansion section 2121, abutment cone head 213, adjustment screw 214, adjustment nut 2141, push rod 22, threaded hole 221, extraction needle tube 3, closing cap 4. DETAILED DESCRIPTION

[0025] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0026] The embodiment is basically as shown in the attached Figures 1 to 3 As shown:

[0027] like Figure 1 - Figure 3 As shown, a nucleic acid extraction device provided in this embodiment can effectively reduce the impact of impurities.

[0028] The present invention provides a nucleic acid extraction device, comprising: a double-chamber component 1, wherein the double-chamber component 1 comprises an extraction cavity tube 11 and an absorption cavity tube 12; wherein the absorption cavity tube 12 is detachably connected to the extraction cavity tube and communicates with the extraction cavity tube; one end of the extraction cavity tube 11 is open and is used to extract nucleic acid; and an absorption component 2, wherein the absorption component 2 is installed in the double-chamber component 1, the absorption component 2 comprises an adjustment piston 21 and a push rod 22, wherein the adjustment piston 21 is installed on the push rod 22, and the adjustment piston 21 reciprocates in the double-chamber component 1 through the push rod 22; the adjustment piston 21 can adjust the diameter; wherein when the nucleic acid mixed solution enters the double-chamber component 1, the push rod 22 is driven to reciprocate, so that the adjustment piston 21 reciprocates up and down. In actual work, the double-chamber 1 structural design adopted in the design can realize convenient separation and further purification effect; the suction piece 2 adopts a variable diameter adjustment piston 21, so that the pressure in the extraction cavity 11 and the suction cavity 12 in the double-chamber 1 can be effectively changed, which can not only realize the pressure difference, but also facilitate the extraction into the suction cavity 12 after stratification, and facilitate the withdrawal of the adjustment piston 21 there, so as to achieve early. At the same time, the use of the double-chamber 1 structural design and the adjustment piston 21 can effectively realize a vacuum environment, thereby preserving the extracted nucleic acid solution.

[0029] In this embodiment, the extraction cavity 11 also has an installation slot 111, which is arranged on the side wall of the extraction cavity 11; the suction cavity 12 also has a fixed block 121, which is arranged on the side wall of the suction cavity 12; wherein the fixed block 121 is detachably connected to the installation slot. In actual use, this design effectively realizes the effective connection and installation and fixation of the two components in the double-chamber 1, namely the extraction cavity 11 and the suction cavity 12.

[0030] In this embodiment, the extraction chamber 11 also has an extraction chamber 112 and a side wall hole 113, the side wall hole 113 is located on the side wall of the extraction chamber 112 and penetrates the mounting slot 111; the suction chamber 12 also has a suction chamber 122 and a connection hole 123, the connection hole 123 is located on the side wall of the suction chamber 122 and penetrates the fixed block 121; wherein the connection hole 123 is connected to the side wall hole 113. In actual use, the purpose of this design is to facilitate the mixed solution of nucleic acids, that is, the mixed liquid in the process of nucleic acid extraction, to enter the suction chamber 12 from the extraction chamber 11, so that separation can be achieved and the purity of nucleic acids can be improved.

[0031] In this embodiment, it also includes: an extraction needle tube 3, an extraction port 114 is provided at one end of the extraction cavity tube 11, and the extraction port 114 is provided at the extraction chamber 112 and communicates with the extraction chamber 112; the extraction needle tube 3 can be detachably installed at the extraction port 114. In actual use, the purpose of this design is to facilitate extraction, and the accessories are available and can be used directly, reducing the design cost.

[0032] In this embodiment, a closing cap 4 is also included, which is detachably mounted on the extraction needle tube 3 and is used to close the extraction chamber 112. In actual use, the purpose of this design is to close the extraction chamber 112, facilitate pressure changes, and make subsequent separation work more thorough.

[0033] In this embodiment, the suction member 2 is provided at two locations, and is respectively installed at the extraction cavity 11 and the suction cavity 12. In actual use, this design effectively realizes the ability to perform reciprocating pressing at the extraction cavity 11 and the suction cavity 12, respectively, so that the separation effect can be accelerated and the purity of nucleic acid can be improved.

[0034] In this embodiment, the adjusting piston 21 includes a sealing abutting rubber sleeve 211 and a reducing block 212; the sealing abutting rubber sleeve 211 is installed on the outer surface of the reducing block 212, and the reducing block 212 is provided with at least one expansion section 2121, wherein the expansion section 2121 is provided at one end of the reducing block 212, and a plurality of expansion sections 2121 are annular and evenly distributed along the center of the reducing block 212. In actual use, the purpose of this design is to achieve diameter reduction and adjust the diameter, so as to adjust the gap between the sealing abutting rubber sleeve 211 and the inner wall of the extraction chamber 112 and the suction chamber 122 during the contact process. In this way, the adjustment can be ensured. Through the expandable expansion section 2121, the expansion section 2121 is annularly arranged, and the expansion section 2121 can be opened and closed to achieve the diameter reduction of the reducing block 212, thereby achieving adjustment.

[0035] In this embodiment, the adjustment piston 21 also includes an abutting cone head 213 and an adjustment screw 214. The abutting cone head 213 is conical, one end of the abutting cone head 213 is connected to one end of the adjustment screw 214, and the other end of the abutting cone head 213 reciprocates in the variable block 212, and the outer wall of the abutting cone head 213 abuts against the expansion section 2121. In actual use, the purpose of this design is to realize the movement of the expansion section 2121 of the variable block 212, so that multiple expansion sections 2121 can be expanded outward after being abutted by force, and the overall opening and closing effect can be achieved, which is essentially similar to opening an umbrella; in this way, the distance between the sealing abutting rubber sleeve 211 outside the expansion section 2121 and the inner wall of the extraction cavity 11 or the suction cavity 12 is changed, and the pressure is adjusted, so that it is convenient to realize the oscillation of the nucleic acid mixture, and then realize the stratification of the nucleic acid mixture, which is convenient for subsequent extraction of nucleic acid.

[0036] In this embodiment, the push rod 22 also has a threaded hole 221, and the threaded hole 221 is arranged along the axial direction of the push rod 22 and passes through the push rod 22; the adjustment screw 214 is rotatably installed in the threaded hole 221, and the other end of the adjustment screw 214 extends outward.

[0037] In this embodiment, the adjusting screw 214 is provided with an adjusting nut 2141 , and the adjusting nut 2141 is disposed at the other end of the adjusting screw 214 and is used to rotate the adjusting screw 214 .

[0038] In actual work;

[0039] First, push the adjustment piston 21 in the suction cavity 12 and keep it still, and press down the adjustment piston 21 at the extraction cavity 11, and first adjust the adjustment nut 2141 on the push rod 22, so that the adjustment piston 21 at the extraction cavity 11 abuts against the extraction chamber 112, and the sealing effect is good; then pull the adjustment piston 21 at the extraction cavity 11, that is, pull the push rod 22 upward;

[0040] In this way, the mixed solution containing nucleic acid is extracted; then the closing cap 4 is used to close the tube mouth of the extraction needle tube 3; the push rod 225 at the extraction cavity tube 11 is reciprocated and pulled 5 times to achieve oscillation of the mixed solution containing nucleic acid, and finally let it stand for a certain period of time; at this time, the push rod 22 is pulled again to make the adjustment piston 21 move upward to above the side wall hole 113 and remain motionless, and the adjustment nut 2141 can also be further rotated to strengthen the sealing between the adjustment piston 21 and the extraction chamber 112; at this time, the adjustment piston 21 at the suction cavity tube 12 is moved upward again, and at this time, preliminary separation can be achieved to purify the mixed solution containing nucleic acid; finally, the closing cap 4 is taken out, and the adjustment piston 21 at the extraction cavity tube 11 is moved downward to achieve its entry into the detection mechanism.

[0041] In summary, this nucleic acid extraction device is not only reasonably designed, but also simple to operate. It can effectively achieve separation, purification and preservation, and effectively solves the adverse effects brought by the above-mentioned impurities. Therefore, this device is suitable for industry promotion.

[0042] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A nucleic acid extraction device, characterized in that: include: A double-chamber component, the double-chamber component comprising an extraction lumen and an aspiration lumen; The suction cavity tube and the extraction cavity tube are detachably connected and communicated with each other; one end of the extraction cavity tube is open and is used to extract nucleic acid; and The suction piece is installed in the double-chamber piece, and the suction piece includes an adjustment piston and a push rod. The adjustment piston is installed on the push rod, and the adjustment piston reciprocates in the double-chamber piece through the push rod; the adjustment piston can change its diameter; when the nucleic acid mixed solution enters the double-chamber piece, the push rod is driven to reciprocate, so that the adjustment piston reciprocates up and down; The adjusting piston comprises a sealing abutting rubber sleeve and a reducing block; the sealing abutting rubber sleeve is installed on the outer surface of the reducing block, and the reducing block is provided with at least one expansion section, wherein the expansion section is provided at one end of the reducing block, and a plurality of expansion sections are annular and evenly distributed along the center of the reducing block; The adjusting piston further comprises an abutting cone head and an adjusting screw, wherein the abutting cone head is conical, one end of the abutting cone head is connected to one end of the adjusting screw, the other end of the abutting cone head reciprocates in the reducing block, and the outer wall of the abutting cone head abuts against the expansion section; The push rod also has a threaded hole, and the threaded hole is arranged along the axial direction of the push rod and passes through the push rod; the adjustment screw is rotatably installed in the threaded hole, and the other end of the adjustment screw extends outward; The adjusting screw is provided with an adjusting nut, and the adjusting nut is arranged at the other end of the adjusting screw and is used to rotate the adjusting screw; The extraction cavity tube also has an installation slot, which is arranged on the side wall of the extraction cavity tube; the suction cavity tube also has a fixed block, which is arranged on the side wall of the suction cavity tube; wherein the fixed block is detachably connected to the installation slot; The extraction chamber tube also has an extraction chamber and a side wall hole, wherein the side wall hole is located on the side wall of the extraction chamber and passes through the mounting slot; the suction chamber tube also has a suction chamber and a connecting hole, wherein the connecting hole is located on the side wall of the suction chamber and passes through the fixed block; wherein the connecting hole is communicated with the side wall hole.

2. A nucleic acid extraction device according to claim 1, characterized in that: Also includes: An extraction needle tube, one end of the extraction cavity tube is provided with an extraction port, and the extraction port is arranged at the extraction chamber and communicated with the extraction chamber; the extraction needle tube is detachably installed at the extraction port.

3. A nucleic acid extraction device according to claim 2, characterized in that: It also includes a closing cap, which is detachably mounted on the extraction needle tube and is used to close the extraction chamber.

4. A nucleic acid extraction device according to claim 2, characterized in that: The suction piece is provided at two locations and is respectively installed at the extraction cavity and the suction cavity.

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