Multi-parameter enzyme reaction equipment based on negative pressure cavitation

Abstract

The utility model discloses multi-parameter enzyme reaction equipment based on negative pressure cavitation, which comprises a raw material container capable of ascending and descending in the vertical direction; the heating mechanism comprises a heating container and a spiral pipe, one end of the spiral pipe is communicated with a first feeding pipe, and the end, away from the spiral pipe, of the first feeding pipe extends into the raw material container; the bottom of the closed enzyme reaction container is communicated with the other end of the coil pipe through a second feeding pipe, two through holes are formed in the top of the enzyme reaction container, one through hole is communicated with a vacuumizing device, the other through hole is communicated with atmosphere control, the bottom of the enzyme reaction container is communicated with a discharging pipe, and the outer wall of the enzyme reaction container is wrapped with a heat preservation layer; and a collecting container is arranged below the discharging pipe. The multi-parameter enzyme reaction equipment based on negative pressure cavitation has the beneficial effects that the negative pressure cavitation technology is combined with enzyme reaction, the enzymolysis efficiency is effectively improved, the energy consumption is reduced, and in addition, the equipment is simple in structure and convenient to clean.

Description

Technical Field

[0001] The utility model relates to the field of enzyme reaction equipment, and more specifically, to a multi-parameter enzyme reaction equipment based on negative pressure cavitation. Background Art

[0002] Enzyme reaction refers to a reaction carried out with enzyme as catalyst. During the catalytic reaction, the enzyme and substrate are fully mixed in the reaction device to catalyze the production of the target product. With the rapid development of biotechnology, bioenzyme reactions are increasingly being used in industrial production. During the enzyme reaction, the raw materials and enzymes need to be fully stirred, and after the enzymatic reaction, the reaction vessel needs to be completely cleaned; negative pressure cavitation is a novel method for extracting active substances. It uses the bubbling cavitation effect under negative pressure to make the sample tissue fully collide and accelerate the exudation efficiency of the active substances in the sample, so as to achieve the purpose of improving efficiency. At the same time, the structure is simple and easy to clean. At present, there is no equipment used to experiment with negative pressure cavitation to promote enzyme reaction binding. Utility Model Content

[0003] One purpose of the utility model is to solve at least the above-mentioned problems and provide a multi-parameter enzyme reaction equipment based on negative pressure cavitation, which combines negative pressure cavitation technology with enzyme reaction, optimizes the system composition, temperature, and cavitation intensity of the enzyme reaction in the same system, thereby effectively improving the enzymatic hydrolysis efficiency while reducing energy consumption. In addition, the equipment has a simple structure and is easy to clean.

[0004] In order to achieve these purposes and other advantages according to the utility model, a multi-parameter enzyme reaction device based on negative pressure cavitation is provided, comprising:

[0005] A raw material container, which can be raised and lowered in the vertical direction;

[0006] A heating mechanism is arranged above the raw material container, the heating mechanism comprises a heating container and a spiral pipe arranged in the heating container, one end of the spiral pipe is connected to a first feeding pipe, one end of the first feeding pipe away from the spiral pipe extends into the raw material container, and a first valve is arranged on the first feeding pipe;

[0007] A closed enzyme reaction container is arranged above the spiral tube, the bottom of the enzyme reaction container is connected to the other end of the coil through a second feed pipe, two through holes are arranged on the top of the enzyme reaction container, one of which is connected to a vacuum pump and the other is connected to atmospheric control, a discharge pipe is connected to the bottom of the enzyme reaction, a second valve is arranged on the discharge pipe, and an insulation layer is arranged on the outer wall of the enzyme reaction container; a collecting container is arranged below the enzyme reaction container and one end of the discharge pipe away from the enzyme reaction container extends into the collecting container.

[0008] Preferably, the heating container is a water bath.

[0009] Preferably, the enzyme reaction container is open at the top and provided with a sealing plug at the open position, and the sealing plug is provided with two of the through holes; it also includes an atmospheric pipe, one end of which is connected to the atmosphere and the other end of which passes through the corresponding through hole and extends into the enzyme reaction container, and a third valve is provided on the tube body of the atmospheric pipe located in the atmosphere.

[0010] Preferably, it further comprises a first thermometer for measuring the enzyme reaction temperature, wherein a measuring end of the first thermometer sealably passes through the sealing plug and then extends into the enzyme reaction container.

[0011] Preferably, the heating container is provided with a second thermometer for measuring the temperature of the reaction liquid in the spiral tube.

[0012] Preferably, the vacuum pump is a vacuum pump, the suction end of the vacuum pump is connected to a suction pipe, and the end of the suction pipe away from the vacuum pump is sealed and passes through a corresponding through hole and then extends into the enzyme reaction container.

[0013] The utility model at least has the following beneficial effects:

[0014] By designing a raw material container, a heating mechanism, a spiral tube, a first feed pipe, a first valve, an enzyme reaction container, a second feed pipe, a through hole, a vacuum device, a discharge pipe, a second valve, an insulation layer, and a collection container, a multi-parameter enzyme reaction equipment based on negative pressure cavitation is provided. The negative pressure cavitation technology is combined with the enzyme reaction to optimize the system composition, temperature, and cavitation intensity of the enzyme reaction in the same system, thereby effectively improving the enzymatic hydrolysis efficiency while reducing energy consumption. The equipment has a simple structure and is easy to clean.

[0015] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of a multi-parameter enzyme reaction device based on negative pressure cavitation according to one of the technical solutions of the utility model.

[0017] Description of the drawings: 1-raw material container; 2-first valve; 3-spiral tube; 4-heating container; 5-second thermometer; 6-first feed pipe; 7-atmospheric pipe; 8-second feed pipe; 9-enzyme reaction container; 10-first thermometer; 11-sealing plug; 12-third valve; 13-suction tube; 14-vacuum pump; 15-pressure gauge; 16-discharge pipe; 17-second valve; 18-collecting container. DETAILED DESCRIPTION

[0018] The present invention will be further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0019] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0020] like Figure 1 As shown, the utility model provides a multi-parameter enzyme reaction device based on negative pressure cavitation, comprising:

[0021] A raw material container 1, which can be raised and lowered in the vertical direction;

[0022] A heating mechanism is arranged above the raw material container 1, and comprises a heating container 4 and a spiral pipe 3 arranged in the heating container 4, one end of the spiral pipe 3 is connected with a first feeding pipe 6, one end of the first feeding pipe 6 away from the spiral pipe 3 extends into the raw material container 1, and a first valve 2 is arranged on the first feeding pipe 6;

[0023] A closed enzyme reaction container 9 is arranged above the spiral tube 3, the bottom of the enzyme reaction container 9 is connected to the other end of the coil through a second feed pipe 8, two through holes are arranged on the top of the enzyme reaction container 9, one of which is connected to a vacuum pump and the other is connected to atmospheric control, a discharge pipe 16 is connected to the bottom of the enzyme reaction, a second valve 17 is arranged on the discharge pipe 16, and an insulation layer is arranged on the outer wall of the enzyme reaction container 9; a collecting container 18 is arranged below the enzyme reaction container 9 and one end of the discharge pipe 16 away from the enzyme reaction container 9 extends into the collecting container 18.

[0024] In the above technical scheme, the raw material container 1 is a container with an open top, which can be a wide-mouth bottle structure, and the structure is not limited. The raw material container 1 can be lifted in the vertical direction in that the raw material container 1 is arranged on a lifting base (not shown in the figure), and the lifting base can be a lifting platform or other lifting equipment commonly used in experiments. The structure of the lifting equipment or the lifting platform is very mature, and no structural limitation is made here, as long as the lifting function can be realized; the heating structure can be a water bath pot, or other heating structure that can hold a heating medium. When the heating container 4 is used, a heating medium (such as water or grease with good thermal insulation effect, etc.) is placed in it, and the spiral tube 3 is provided In the heating container 4, when in use, the spiral tube 3 is immersed in the heating medium of the heating container 4. The structure of the spiral tube 3 is a common spiral tube structure (refer to the condenser tube). The spiral tube 3 is fixed in the heating container 4 by a support rod or a bracket (not shown in the figure) (for example, a pair of support rods are fixedly connected to the outer wall of the spiral tube 3, and the other end of the pair of support rods is fixedly connected to the heating container 4 or detachably connected, etc.), and the spiral tube 3 has two ports, namely a feed end and a discharge end. The feed end is connected to a first feed pipe 6. The end of the first feed pipe 6 away from the spiral tube 3 extends into the raw material container 1. By lifting the raw material container 1, the first feed pipe 6 can be immersed in the reaction liquid or detached. The reaction liquid is connected with a second feed pipe 8 at the discharge end, and the end of the second feed pipe 8 away from the spiral tube 3 is connected to the bottom of the enzyme reaction container 9. The enzyme reaction container 9 is arranged above the heating container 4. The bottom of the enzyme reaction container 9 is also connected with a discharge pipe 16. A collecting container 18 is arranged below the enzyme reaction container 9. The collecting container 18 is a container with an open top. The end of the discharge pipe 16 away from the enzyme reaction container 9 extends into the collecting container 18. A second valve 17 is arranged on the discharge pipe 16, and the second valve 17 is used to control whether the enzyme reaction container 9 discharges into the collecting container 18; the top of the enzyme reaction container 9 is opened. There are two through holes, which are the first through hole and the second through hole respectively. The first through hole corresponds to the atmosphere, and the second through hole corresponds to the vacuum pumping device. The first through hole is controlled to be connected to the atmosphere. The specific implementation method can be: a valve for controlling the opening and closing of the first through hole is provided at the first through hole, and the valve can be used to control whether the first through hole is connected to the atmosphere, thereby controlling whether the inside of the enzyme reaction container 9 is connected to the atmosphere; the second through hole is connected to the vacuum pumping device to promote the formation of negative pressure inside the enzyme reaction container 9; the material of the thermal insulation layer is selected according to the actual situation, and the enzyme reaction container 9 can also be set to a thermos bottle structure; it should be noted that the attached Figure 1 It is only a schematic diagram of the principle structure. In actual production and preparation, relevant auxiliary structures can be added according to actual conditions, such as supporting structures (such as brackets) for supporting the enzyme reaction container 9, the collection container 18, the heating mechanism, etc.;

[0025] In this technical solution, the specific use process is as follows: add the reaction liquid into the raw material container 1 so that the liquid inlet end of the first feeding pipe 6 (the liquid inlet end is the end extending into the raw material container 1) is immersed in the reaction liquid, and then add a medium (such as water or grease with good heat preservation effect) into the heating container 4, start the heating device, make the heating container 4 reach the set temperature, then open the first valve 2, close the second valve 17 and close the through hole connecting the enzyme reaction container 9 with the atmosphere, start the vacuum device, and pass the negative electrode through the enzyme reaction container 9, the second feeding pipe 8, and the spiral tube 3. The pressure is transmitted to the liquid inlet end of the first feed pipe 6, and a relatively low negative pressure is maintained, so that the reaction liquid slowly enters the enzyme reaction container 9. In the process of the reaction liquid entering the enzyme reaction container 9, the reaction liquid slowly enters the spiral tube 3 and is heated by the medium in the heating container 4. Under the slow speed of the reaction liquid and the long path of the spiral tube 3, the reaction liquid can be heated more comprehensively. When the liquid level in the enzyme reaction container 9 is about to reach a safe height, the raw material container 1 is lowered to cause the first feed pipe 6 to be separated from the reaction liquid and placed in the air, and then the negative pressure of the vacuum pump is increased to reach the set pressure. At this time, under the protection of the insulation layer, the reaction liquid in the enzyme reaction container 9 reacts within a certain temperature range, and the air also enters the enzyme reaction container 9 through the first feed pipe 6 and the spiral tube 3 to form negative pressure cavitation, thereby improving the enzyme reaction effect. When the set reaction time is reached, the heating of the heating container 4 is stopped, and the first valve 2 is quickly closed to connect the through hole at the top of the enzyme reaction container 9 to the atmosphere, and the pressure of the vacuum pump 14 is gradually lowered to close the vacuum pump 14. At this time, the inside of the enzyme reaction container 9 is connected to the atmosphere, and then the second valve 17 is opened. , under the action of gravity, the reaction liquid in the enzyme reaction container 9 enters the collecting container 18 through the discharge pipe 16. After the reaction liquid in the enzyme reaction container 9 is emptied, the second valve 17 can be closed, and the first valve 2 can be opened to discharge the reaction liquid in the spiral tube 3 from the first feeding pipe 6 (collect the reaction liquid in the spiral tube 3). In actual use, the reaction liquid remaining in the first feeding pipe 6 can be removed as needed; when the equipment needs to be cleaned, the reaction liquid can be replaced with clean water or safe detergent according to the above process (the heating mechanism can be turned on as needed);

[0026] The technical solution has the beneficial effect that, by designing the raw material container 1, the heating mechanism, the spiral tube 3, the first feed tube 6, the first valve 2, the enzyme reaction container 9, the second feed tube 8, the through hole, the vacuum device, the discharge tube 16, the second valve 17, the insulation layer, and the collection container 18, a multi-parameter enzyme reaction device based on negative pressure cavitation is provided, the negative pressure cavitation technology is combined with the enzyme reaction, the system composition, temperature, and cavitation intensity of the enzyme reaction are optimized in the same system, thereby effectively improving the enzymatic hydrolysis efficiency while reducing energy consumption, and the structure of the device is simple and easy to clean; in addition, the spiral tube 3 The setting provides a long-path pipeline for the reaction liquid in the heating container 4, which can not only increase the total reaction liquid volume of the reaction liquid (the reaction liquid in the enzyme reaction container 9 plus the reaction liquid in the spiral tube 3, all belong to the reaction liquid after the reaction), but also the long path of the spiral tube 3 ensures that the path for air to enter the enzyme reaction container 9 is relatively long, which can ensure that the air enters the enzyme reaction container 9 to form a stable partial negative pressure cavitation phenomenon, and avoid the short path (such as directly connecting the first feeding pipe 6 with the bottom of the spiral tube 3), the air is directly sucked away at the suction end, ensuring the stability of the negative pressure cavitation during the reaction of the enzyme reaction container 9.

[0027] In another technical solution, the heating container 4 is a water bath pot; a water bath pot is a common device and is easy to purchase and use.

[0028] In another technical solution, the enzyme reaction container 9 is open at the top and a sealing plug 11 is provided at the open portion, and the sealing plug 11 is provided with two of the through holes; it also includes an atmospheric pipe 7, one end of the atmospheric pipe 7 is connected to the atmosphere, and the other end passes through the corresponding through hole and extends into the enzyme reaction container 9, and a third valve 12 is provided on the tube body of the atmospheric pipe 7 located in the atmosphere; specifically, the enzyme reaction container 9 can be a wide-mouth bottle structure, which is open at the top and a sealing plug 11 is provided to seal the open portion, and the sealing plug 11 is provided with two of the through holes, namely a first through hole and a second through hole, and the atmospheric pipe 7 is sealed on the first through hole (the sealing method can be that the sealing plug 11 is elastically plugged into a rubber The inner diameter of the first through hole on the sealing plug 11 is smaller than the outer diameter of the atmospheric pipe 7. When the atmospheric pipe 7 passes through the first through hole, the elasticity of the sealing plug 11 causes the atmospheric pipe 7 to be connected and sealed with the sealing plug 11). One end of the atmospheric pipe 7 is located in the enzyme reaction container 9 and suspended above the reaction liquid surface, and the other end is in the atmosphere (air). A third valve 12 is provided on the tube body of the atmospheric pipe 7 in the air. By controlling the closing and opening of the third valve 12, the connection between the inside of the enzyme reaction container 9 and the atmosphere can be controlled. The technical solution has the beneficial effect that, by arranging the sealing plug 11 and the atmospheric pipe 7, a method for sealing the top of the enzyme reaction container 9 and connecting it to the atmosphere is provided, which is convenient for operation and use.

[0029] In another technical solution, it also includes a first thermometer 10 for measuring the enzyme reaction temperature. The measuring end of the first thermometer 10 is sealed and passes through the sealing plug 11 and then extends into the enzyme reaction container 9. Specifically, the measuring end of the first thermometer 10 is sealed and passes through the sealing plug 11 and then extends into the enzyme reaction container 9 and is located below the reaction liquid surface. The enzyme reaction container 9 can be transparent, the thermometer can be a mercury thermometer or the enzyme reaction container 9 is opaque, and the end of the thermometer located in the atmosphere (air) is the temperature display end, which is selected according to actual conditions. The technical solution has the beneficial effect that, by designing the first thermometer 10, the temperature of the reaction liquid in the enzyme reaction container 9 can be measured in real time, which is convenient for the operator to make timely adjustments according to abnormal temperature.

[0030] In another technical solution, the heating container 4 is provided with a second thermometer 5 for measuring the temperature of the reaction liquid in the spiral tube 3. Specifically, the measuring end of the second thermometer 5 is sealed and extends into the spiral tube 3 to measure the temperature of the reaction liquid in the spiral tube 3. When in use, the second thermometer 5 can measure the temperature of the reaction liquid in real time, which is convenient for the operator to monitor the temperature of the reaction liquid in the spiral tube 3, and then adjust the temperature of the heating container 4 and other operations to ensure the smooth progress of the enzyme reaction.

[0031] In another technical solution, the vacuum pump 14 is a vacuum pump 14, and the suction end of the vacuum pump 14 is connected to a suction pipe 13, and the end of the suction pipe 13 away from the vacuum pump 14 is sealed through the corresponding through hole and then extends into the enzyme reaction container 9; specifically, the vacuum pump 14 is a common device, and a pressure gauge 15 is provided on the vacuum pump 14. The suction end of the vacuum pump 14 is connected to a suction pipe 13, and the other end of the suction pipe 13 is sealed through the second through hole on the sealing plug 11 and then extends into the enzyme reaction container 9, suspended above the reaction liquid level (that is, when in use, the safe height of the reaction liquid level must be lower than the end of the suction pipe 13 away from the vacuum pump 14); the technical solution has the beneficial effect that, by designing the vacuum pump 14 and the suction pipe 13, a method for evacuating the inside of the enzyme reaction container 9 is provided, so that a negative pressure state can be formed inside the enzyme reaction container 9, and the vacuum pump 14 has a wide source and is relatively practical.

[0032] The utility model provides a multi-parameter enzyme reaction device based on negative pressure cavitation, comprising:

[0033] A raw material container 1, which can be raised and lowered in the vertical direction;

[0034] A heating mechanism is arranged above the raw material container 1, and comprises a heating container 4 and a spiral pipe 3 arranged in the heating container 4, one end of the spiral pipe 3 is connected with a first feeding pipe 6, one end of the first feeding pipe 6 away from the spiral pipe 3 extends into the raw material container 1, and a first valve 2 is arranged on the first feeding pipe 6;

[0035] A closed enzyme reaction container 9 is arranged above the spiral tube 3, the bottom of the enzyme reaction container 9 is connected to the other end of the coil through the second feed pipe 8, the top of the enzyme reaction container 9 is open and a sealing plug 11 is provided at the open position, the sealing plug 11 is provided with two through holes, one of which is connected to the vacuum device; it also includes an atmospheric pipe 7, one end of the atmospheric pipe 7 is sealed and extends into the enzyme reaction container 9 through the other through hole, and the other end is located in the atmosphere, and a third valve 12 is provided on the tube body of the atmospheric pipe 7 located in the atmosphere; the bottom of the enzyme reaction is connected to a discharge pipe 16, the discharge pipe 16 is provided with a second valve 17, and the outer wall of the enzyme reaction container 9 is wrapped with a heat-insulating layer; a collecting container 18 is provided below the enzyme reaction container 9, and the end of the discharge pipe 16 away from the enzyme reaction container 9 extends into the collecting container 18;

[0036] The heating container 4 is a water bath;

[0037] It also includes a first thermometer 10 for measuring the enzyme reaction temperature, wherein the measuring end of the first thermometer 10 is sealed through the sealing plug 11 and then extends into the enzyme reaction container 9;

[0038] The heating container 4 is provided with a second thermometer 5 for measuring the temperature of the reaction liquid in the spiral tube 3;

[0039] The vacuum pump 14 is a vacuum pump. The suction end of the vacuum pump 14 is connected to a suction pipe 13. The end of the suction pipe 13 away from the vacuum pump 14 is sealed and passes through a corresponding through hole and then extends into the enzyme reaction container 9.

[0040] In the above technical scheme, when in use, the reaction liquid is added to the raw material container 1 so that the liquid inlet end of the first feeding pipe 6 (the liquid inlet end is the end extending into the raw material container 1) is immersed in the reaction liquid, and then water is added to the water bath, the heating device is started, and the heating container 4 reaches the set temperature, and then the first valve 2 is opened, the second valve 17 and the third valve 12 are closed, and the vacuum pump 14 is started. The negative pressure is transmitted to the liquid inlet end of the first feeding pipe 6 through the enzyme reaction container 9, the second feeding pipe 8, and the spiral tube 3, and a relatively low negative pressure is maintained, so that the reaction liquid slowly enters the enzyme reaction container 9. In the process of the reaction liquid entering the enzyme reaction container 9, the reaction liquid slowly enters the spiral tube 3 and is heated by the heating container 4. Under the slow speed of the reaction liquid and the long path of the spiral tube 3, the reaction liquid can be heated more comprehensively. When the liquid level in the enzyme reaction container 9 is about to reach a safe height, the raw material container 1 is lowered to cause the first feeding pipe 6 to be separated from the reaction liquid and placed in the air, and then the negative pressure of the vacuum pump 14 is increased to reach the set pressure. At this time, the reaction liquid in the heat preservation layer Under protection, the reaction liquid in the enzyme reaction container 9 reacts within a certain temperature range, and air also enters the enzyme reaction container 9 through the first feed pipe 6 and the spiral tube 3 to form negative pressure cavitation, thereby improving the enzyme reaction effect. When the set reaction time is reached, the water bath is stopped from heating, the first valve 2 is quickly closed, the third valve 12 is opened, and the pressure of the vacuum pump 14 is gradually reduced to close the vacuum pump 14. At this time, the interior of the enzyme reaction container 9 is connected to the atmosphere, and then the second valve 17 is opened. Under the action of gravity, the reaction liquid in the enzyme reaction container 9 enters the collecting container 18 through the discharge pipe 16. After the reaction liquid in the enzyme reaction container 9 is emptied, the second valve 17 can be closed, and the first valve 2 can be opened to discharge the reaction liquid in the spiral tube 3 from the first feed pipe 6 (collect the reaction liquid in the spiral tube 3). In actual use, the reaction liquid remaining in the first feed pipe 6 can be removed as appropriate; when the equipment needs to be cleaned, the reaction liquid can be replaced with clean water or safe detergent according to the above process (the heating mechanism can be started as appropriate);

[0041] The beneficial effect obtained by adopting this technical solution is that a multi-parameter enzyme reaction equipment based on negative pressure cavitation is provided, the negative pressure cavitation technology is combined with the enzyme reaction, the system composition, temperature and cavitation intensity of the enzyme reaction are optimized in the same system, thereby effectively improving the enzymolysis efficiency while reducing energy consumption, and the structure of the equipment is simple and easy to clean; in addition, the setting of the spiral tube 3 provides a long-path pipeline for the reaction liquid in the heating container 4, which can not only increase the total reaction liquid volume of the reaction liquid (the reaction liquid in the enzyme reaction container 9 plus the reaction liquid in the spiral tube 3, both belong to the reaction liquid after the reaction), but also the long path of the spiral tube 3 ensures that the path for air to enter the enzyme reaction container 9 is relatively long, so that the air can enter the enzyme reaction container 9 to form a stable negative pressure cavitation phenomenon, avoiding the short path (such as directly connecting the first feeding pipe 6 with the bottom of the spiral tube 3), the air is directly sucked away at the suction end, ensuring the stability of the negative pressure cavitation during the reaction of the enzyme reaction container 9.

[0042] The number of devices and processing scales described here are used to simplify the description of the utility model. The application, modification and variation of the multi-parameter enzyme reaction device based on negative pressure cavitation of the utility model are obvious to those skilled in the art.

[0043] Although the implementation scheme of the utility model has been disclosed as above, it is not limited to the applications listed in the specification and implementation modes. It can be fully applied to various fields suitable for the utility model. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the utility model is not limited to the specific details and the illustrations shown and described herein.

Claims

1. Multi-parameter enzyme reaction equipment based on negative pressure cavitation, characterized in that: include: A raw material container, which can be raised and lowered in the vertical direction; A heating mechanism is arranged above the raw material container, the heating mechanism comprises a heating container and a spiral pipe arranged in the heating container, one end of the spiral pipe is connected to a first feeding pipe, one end of the first feeding pipe away from the spiral pipe extends into the raw material container, and a first valve is arranged on the first feeding pipe; A closed enzyme reaction container is arranged above the spiral tube, the bottom of the enzyme reaction container is connected to the other end of the coil through a second feed pipe, two through holes are arranged on the top of the enzyme reaction container, one of which is connected to a vacuum pump and the other is connected to atmospheric control, a discharge pipe is connected to the bottom of the enzyme reaction, a second valve is arranged on the discharge pipe, and an insulation layer is arranged on the outer wall of the enzyme reaction container; a collecting container is arranged below the enzyme reaction container and one end of the discharge pipe away from the enzyme reaction container extends into the collecting container.

2. The multi-parameter enzyme reaction device based on negative pressure cavitation according to claim 1, characterized in that: The heating container is a water bath.

3. The multi-parameter enzyme reaction device based on negative pressure cavitation according to claim 1, characterized in that: The enzyme reaction container is open at the top and is provided with a sealing plug at the open position, and the sealing plug is provided with two through holes; it also includes an atmospheric pipe, one end of which is connected to the atmosphere, and the other end of which passes through the corresponding through hole and extends into the enzyme reaction container, and a third valve is provided on the tube body of the atmospheric pipe located in the atmosphere.

4. The multi-parameter enzyme reaction device based on negative pressure cavitation according to claim 3, characterized in that: It also includes a first thermometer for measuring the enzyme reaction temperature. The measuring end of the first thermometer is sealed through the sealing plug and then extends into the enzyme reaction container.

5. The multi-parameter enzyme reaction device based on negative pressure cavitation according to claim 1, characterized in that: The heating container is provided with a second thermometer for measuring the temperature of the reaction liquid in the spiral tube.

6. The multi-parameter enzyme reaction device based on negative pressure cavitation according to claim 3, characterized in that: The vacuum pump is a vacuum pump. The suction end of the vacuum pump is connected with a suction pipe. The end of the suction pipe away from the vacuum pump is sealed and passes through the corresponding through hole and then extends into the enzyme reaction container.

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