Immunofluorescent staining mold filter element

Abstract

The utility model discloses an immunofluorescent staining mould filter, which belongs to the technical field of experimental supplies and comprises a plurality of plate frames and a plurality of filter screen cups, the filter screen cups are distributed on the plate frames in a matrix manner, and the plate frames can be respectively placed in a plurality of liquid holding holes arranged on a pore plate mould in a one-to-one correspondence manner. The filter element of the immunofluorescence staining mold is simple in structure and convenient to operate, and can solve the problems that multiple groups of slices are difficult to take and place, time-consuming and labor-consuming and are easy to damage.

Description

Technical Field

[0001] The utility model belongs to the field of experimental supplies and provides an immunofluorescence staining mold filter. Background Art

[0002] Traumatic Brain Injury (TBI) is the most common injury in neurological diseases and poses a serious threat to human life. Its disability rate, morbidity rate and mortality rate remain high and have an upward trend every year. It is one of the diseases with high morbidity and mortality in the world. Neurological damage caused by TBI includes primary injury and secondary brain injury. Secondary injury often occurs hours or days after the injury, including brain edema, intracranial hypertension, etc. Brain edema is an important secondary injury of TBI. It has a rapid onset and rapid progression. It can form serious fatal complications such as brain herniation and is a key factor that endangers life. Through research, it is speculated that early peritoneal dialysis after brain trauma can control the degree of brain edema. Its role is to reduce the plasma glutamate level through PD, thereby indirectly reducing the abnormal accumulation of glutamate in the interstitial space of brain tissue after TBI, reducing the cytotoxic brain edema caused by the swelling of astrocytes in the brain tissue, improving the blood supply and oxygen supply of the brain tissue, and blocking the vicious cycle of intracranial hypertension, thereby reducing the mortality rate of TBI patients and improving the prognosis of patients.

[0003] It is planned to establish a rat TBI model combined with a rat PD model to explore whether peritoneal dialysis treatment in the early stage after TBI can regulate the degree of brain edema, and provide a research basis for the treatment of brain edema in clinical TBI patients. During the experiment, immunofluorescence staining was used to detect the expression of Glu and inflammatory mediators in the brain. Immunohistochemistry (immunofluorescence staining) uses the principle of specific binding of antigens and antibodies to determine the location, qualitative and quantitative research of antigens in tissue cells by making the labeled color developer develop color through chemical reactions. The immunohistochemical steps include: taking out the frozen sections, leaving them at room temperature to dry, and then fixing them with cold acetone at 4°C for 10 minutes; then, washing the sections with 0.01M PBS and adding 1.2% hydrogen peroxide for 30 minutes to remove non-specific staining; then, washing with 0.01M PBS for 3 times × 10 minutes; then using 0.3% Triton X-100 for 30 minutes; then, adding the primary antibody diluted to the working concentration with antibody diluent (0.01M PBS containing 1% BSA, pH7.4), and incubating at 4°C overnight; then washing with 0.01M PBS for 3 times × 10 minutes; then, adding fluorescent antibodies TRITC-IgG (1:100) or FITC-IgG (1:100), incubating at room temperature for 2 hours; then washing with 0.01M PBS for 3 times × 10 minutes; finally, covering the sections with buffered glycerol and observing under a fluorescence microscope. As mentioned above, well plate molds 5 (such asFigure 1 The invention discloses a filter element for frozen section immunofluorescence staining well plate mold, which is a plan view schematic diagram shown in FIG. 1 , and it is necessary to take out and put in the liquid holding hole 6 of the well plate mold many times, which is easy to damage the slices, and the process of taking and putting the slices is laborious, and it also takes a long time and energy. Therefore, it is necessary to develop a filter element for frozen section immunofluorescence staining well plate mold, so as to achieve the purpose of quickly taking and putting in multiple groups of slices without damaging them. Utility Model Content

[0004] In view of this, the purpose of the utility model is to provide an immunofluorescence staining mold filter with a simple structure and easy operation, aiming to solve the problem that it is difficult to take and place multiple groups of slices, which is time-consuming, laborious and easy to be damaged.

[0005] In order to achieve the above object, the utility model provides the following technical solutions:

[0006] The utility model provides an immunofluorescence staining mold filter, including a plate frame and a filter cup, wherein the filter cup is arranged in a plurality and distributed in a matrix on the plate frame, and the plurality of plate frames can be placed one by one in a plurality of liquid holding holes arranged on the orifice plate mold. Immunofluorescence staining experiments can be performed. The filter cup has a specific pore size and number of holes, and can be customized according to experimental requirements. The material of the filter cup is usually selected from corrosion-resistant, high-temperature-resistant and transparent materials, such as polycarbonate (PC) or polypropylene (PP). This immunofluorescence staining mold filter can improve experimental efficiency.

[0007] Furthermore, the height of the filter cup is close to the depth of the liquid holding hole, and the outer diameter of the filter cup is close to the inner diameter of the liquid holding hole, so that the filter cup can be effectively placed in the liquid holding hole.

[0008] Furthermore, the plate frame is composed of a crossbar and a crossarm. A plurality of crossarms are arranged side by side and spaced between two parallel crossbars, and the filter cup is arranged on the crossarm. The crossbar, as the main supporting part of the frame, bears the weight and pressure of the entire structure. The crossarm serves to connect and strengthen the crossbars. By arranging them side by side and at intervals, the stability and strength of the entire structure can be increased. The filter cup is placed on the crossarm, which can fully utilize the supporting function of the crossarm to ensure the stability and reliability of the filter cup.

[0009] Furthermore, the cross arm is slidably arranged on the cross bar, and the filter cup is slidably arranged on the cross arm. This design allows the cross arm to slide horizontally on the cross bar, so that the position of the filter cup can be easily adjusted. By moving the cross arm, the position of the filter cup relative to the cross bar can be changed to adapt to different operating requirements. The advantage of the filter cup sliding on the cross arm is that the position of the filter cup can be adjusted as needed to facilitate operation and maintenance. This design is usually used in scenarios where the filter cup needs to be replaced frequently or different operations need to be performed, providing greater flexibility and convenience.

[0010] Furthermore, the panel frame also includes a handle, and the same side ends of the two cross bars are respectively arranged on the handle. This design makes the handle an integral part of the entire panel frame structure. The arrangement of the handle can conveniently manipulate and move the panel frame without directly touching the cross bar or cross arm. At the same time, by arranging the same side ends of the two cross bars on the handle, the stability and balance of the cross bar can be ensured.

[0011] Furthermore, the crossbar is plug-connected to the handle. This design allows the crossbar to be easily inserted into the handle and to be stabilized by the plug-in connection. The plug-in connection is usually achieved by designing the shapes and sizes of the crossbar and the handle so that they can fit each other to achieve a reliable connection.

[0012] Further, the cross arm slides in or out from the end of the cross bar away from the handle. This design allows the cross arm to slide in or out from the end of the cross bar away from the handle conveniently to adapt to different operating requirements.

[0013] The beneficial effects of the utility model are:

[0014] 1. Easy to operate: By operating the plate frame, multiple groups of filter cups can be taken and placed as a whole, making the operation more convenient. There is no need to take and place the filter cups one by one, thus saving time and energy.

[0015] 2. One-time operation for cleaning and placing multiple groups of slices: Since the number of liquid holding holes of multiple groups of filter cups matches that of the orifice plate mold, multiple groups of slices can be cleaned and placed at one time, reducing the number of operations and improving efficiency.

[0016] 3. Reduce slice damage: The overall design of the filter cup can reduce the friction and collision of slices during the process of taking and placing, thereby reducing the risk of slice damage.

[0017] In general, through the design of the plate-frame structure, the utility model can simplify the operating steps, improve the operating efficiency, and reduce the risk of slice damage, thereby bringing a series of benefits, including saving time and energy, and improving work efficiency.

[0018] Other advantages, objectives and features of the present invention will be described in the following description to some extent, and will be apparent to those skilled in the art based on the following examination and research, or can be taught from the practice of the present invention to some extent. The objectives and other advantages of the present invention can be achieved and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model will be described in detail below in conjunction with the accompanying drawings, in which:

[0020] Figure 1 It is a schematic diagram of the plane structure of an existing orifice plate mold;

[0021] Figure 2 This is a schematic structural diagram of the immunofluorescence staining mold filter of the utility model;

[0022] Figure 3 It is a schematic diagram of the planar structure of a well plate mold suitable for the immunofluorescence staining mold filter of the utility model;

[0023] Figure numerals: cross bar 1, cross arm 2, filter cup 3, handle 4, orifice plate mold 5, liquid holding hole 6, and give way groove 7. DETAILED DESCRIPTION

[0024] The utility model is further described below in conjunction with specific implementation methods. The drawings are only used for exemplary descriptions, and are only schematic diagrams, not actual pictures, and cannot be understood as limitations on this patent; in order to better illustrate the embodiments of the utility model, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0025] like Figure 2 As shown, the immunofluorescence staining mold filter mentioned in this embodiment includes a plate frame and a filter cup 3. The filter cup 3 is arranged in multiple numbers and distributed in a matrix on the plate frame, and the multiple plate frames can be placed in a one-to-one correspondence in multiple liquid holding holes 6 arranged on the orifice plate mold 5, and the plate frame is composed of a crossbar 1 and a crossarm 2. The crossarm 2 is arranged in multiple numbers and is arranged side by side and spaced between two parallel crossbars 1, and the filter cup 3 is arranged on the crossarm 2. In this way, the filter cup and the plate frame are an integral structure. When in use, the slices are placed in the filter cup, and the plate frame is operated so that the multiple groups of filter cups thereon are taken out and placed from the liquid holding holes as a whole. The operation and use are convenient, and the purpose of washing and taking out multiple groups of slices in one operation is achieved.

[0026] In this embodiment, the height of the filter cup 3 is close to the depth of the liquid holding hole 6, and the outer diameter of the filter cup 3 is close to the inner diameter of the liquid holding hole 6. This ensures the normal cleaning of the slices and the effective operation of the filter cup.

[0027] In another embodiment, the cross arm 2 is slidably arranged on the cross bar 1, and the filter cup 3 is slidably arranged on the cross arm 2. In this way, the respective sliding arrangements can be adapted to different liquid-receiving holes 6 arranged on the orifice plate mold 5, such as 3x4, 4x4, 4x6, etc. The sliding arrangement can be a relatively tight fit relationship, which can achieve a relative movement effect and also have a relative fixed effect.

[0028] In another embodiment, the plate frame further includes a handle 4, and the same side ends of the two cross bars 1 are respectively arranged on the handle 4, and the cross bars 1 are plugged and connected with the handle 4. In this way, it is convenient to operate the entire plate frame through the handle. At the same time, the cross arm 2 slides in or out from the end of the cross bar 1 away from the handle 4 to facilitate the adjustment and setting of the number of filter cups on the cross arm. In addition, in order to make the plate frame compatible with the orifice plate mold, a recess 7 for placing the plate frame can be processed on the upper surface of the orifice plate mold, such as Figure 3 shown.

[0029] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution, which should be included in the scope of the claims of the utility model.

Claims

1. An immunofluorescence staining mold filter, characterized in that: The invention comprises a plate frame and a filter cup (3), wherein the filter cup is arranged in a plurality and distributed in a matrix on the plate frame, and the plurality of plate frames can be placed in a plurality of liquid holding holes arranged on the orifice plate mold in a one-to-one correspondence; the plate frame is composed of a cross bar (1) and a cross arm (2), wherein the cross arm is arranged in a plurality and arranged in a side-by-side manner between two parallel cross bars, and the filter cup is arranged on the cross arm; the cross arm is slidably arranged on the cross bar, and the filter cup is slidably arranged on the cross arm; the plate frame also comprises a handle (4), and the same side ends of the two cross bars are arranged on the handle; the cross arm slides in or out from the end of the cross bar away from the handle.

2. The immunofluorescence staining mold filter according to claim 1, characterized in that: The height of the filter cup is close to the depth of the liquid holding hole, and the outer diameter of the filter cup is close to the inner diameter of the liquid holding hole.

3. The immunofluorescence staining mold filter according to claim 1, characterized in that: The cross bar is plug-connected with the handle.

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