The invention relates to a pixel structure, a display substrate and a display device. The pixel structure comprises a plurality of first pixels, second pixels and third pixels, wherein each first pixel comprises a first sub-pixel which is arranged on the right upper side and a second sub-pixel which is arranged on the left lower side, each second pixel comprises a second sub-pixel which is arranged on the right upper side and a third sub-pixel which is arranged on the left lower side, each third pixel comprises a third sub-pixel which is arranged on the right upper side and a first sub-pixel which is arranged on the left lower side, the second pixel is arranged above the right side of the first pixel, the third pixel is arranged above the right side of the second pixel, and the first pixel is arranged above the right side of the third pixel. By adopting the technical scheme, when the pixel structure displays an image with an oblique boundary, a transverse boundary and/or a vertical boundary, the boundary of the image is formed by alternately arranging the first sub-pixel, the second sub-pixel and the third sub-pixel, and the color crisperding phenomenon can be avoided.