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This invention belongs to the field of microbial technology, and relates to a probiotic agent containing *Lactobacillus rhamnosus* CL-03 for relieving eczema, its preparation method, and its application. The strain in the agent is *Bifidobacterium longum* subsp. *infantile* with accession number GDMCC No:64541. Bifidobacterium longum subsp.infantis Strain C-3 and Lactobacillus rhamnosus with accession number GDMCC No. 65725 Lactobacillus rhamnosus Composition of CL-03 strain. The probiotic agent prepared by this invention can relieve eczema, repair the skin barrier, and inhibit the proliferation of harmful bacteria on the skin. Moreover, the Bifidobacterium longum subsp. infantis C-3 strain and Lactobacillus rhamnosus CL-03 strain have a certain synergistic effect on the above-mentioned effects.