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Tissue-culturing and seedling-growing method for rosa canina

A tissue culture and rose technology, which is applied in the fields of botany equipment and methods, horticultural methods, plant cells, etc., can solve the problems that the seedlings are difficult to maintain the excellent traits of the female parent and are prone to variation, and achieve the effect of realizing large-scale production.

Inactive Publication Date: 2007-12-19
江苏春辉生态农林股份有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that it is difficult for the seedlings to maintain the excellent traits of the female parent, and it is easy to mutate.

Method used

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Embodiment Construction

[0016] The present invention relates to a method for tissue culture and seedling cultivation of Rosa canida, which comprises: explant material extraction, primary culture, subculture, rooting culture and transplanting of test-tube seedlings in six major process steps, the specific process steps are as follows:

[0017] Step 1. Extracting materials for explants. During the continuous sunny days from April to May, cut the annual semi-lignified branches of Rosa dogii from the field, cut off the leaves, and cut them into 2 to 3 stem segments. Shake and wash for 6-8 minutes, then rinse with running water under the tap for 30±5 minutes, blot the water with clean gauze, and then place it in a sterile ultra-clean workbench and disinfect it in 7075% ethanol solution for 30±5 seconds. Rinse with sterile water for 3 to 4 times, then transfer to 0.08 to 0.12% HgCl 2 Disinfect in the solution for 8 to 10 minutes, and finally rinse with sterile water for 5 to 6 times, each time for 4±2 minu...

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Abstract

The invention relates to the tissue culture of Dog hedge rose and the breeding method, belong to fields of plant cultivation, plant asexual breeding and gardening, the detail steps of which are: getting annual Dog hedge rose branch from field, cutting off the leaves, slicing into 2-3 stem segments, then slicing into single stem segment of single sprout, inoculating in the first generation culture medium, the single sprout in the single stem segment grow to bud sprout, cutting off the bud sprout from the stem segment and transferring into the closed vessel equipped with successive culture medium for successive culture, breeding tube sprout, cutting the bud sprout with leaves more than three into single bud stem segment during successive culture, and cutting bud sprout with leaves lees than three into single plant, carrying out the same steps for every 30+ / -5 days, getting out the breeding tube sprout and transferring into the closed vessel equipped with root-growing culture medium for root growing culture, replanting to the base material in the end, getting the nursery-grown plant. The invention is characterized by the high breeding coefficient, high survival rate, and no deseription variation of Dog hedge rose, which can solve the mass producing problem and promote the mass production of tree-shaped Chinese rose.

Description

Technical field: [0001] The invention relates to a method for tissue culture and seedling cultivation of plants of the genus Rosa, in particular to a method for tissue culture and seedling cultivation of Rosa canina. It belongs to the technical fields of plant cultivation, plant asexual propagation and horticulture. Background technique: [0002] Dog rose belongs to the family Rosaceae Rosa, which is a traditional fine tree rose rootstock in Europe. It has straight stems, high hardness, pit wind, strong cold resistance, and wide adaptability. Grafting rose has good affinity and has a broad market prospect. Before the present invention was made, the propagation methods of traditional dog rose had three kinds: seed propagation, cutting propagation and leaf in vitro propagation. The dormant time of seed propagation is long, and the annual germination rate of sand storage is only 5% to 10%, which has no application value in production. Cutting propagation is carried out by g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G1/00A01G7/00
Inventor 吴有光曹亮顾海燕
Owner 江苏春辉生态农林股份有限公司