Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Spleen extracts, its preparation method and use

A technology of extract and spleen, which is applied in the field of pharmaceutical preparations and achieves the effects of exact curative effect, low cost and low cost

Active Publication Date: 2008-12-10
融致丰生制药有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As we all know, there are many nutrients and active ingredients needed by the human body in mammals, and there are many reports on the extraction of active peptides from the heart, liver and muscle tissue of mammals

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This example provides a spleen extract containing 8.0 mg of polypeptide, 10.2 mg of free amino acid, 2.2 mg of nucleic acid, 260 μg of total sugar and molecular weight less than 6000 Dalton per ml. Its preparation steps are as follows:

[0053] A) Take 10kg of fresh healthy calf spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 10L water for injection, homogenate, adjust the pH value to 3.8, freeze at -20°C, take out after freezing and place in a water bath at 15°C The homogenate after the last thaw was centrifuged at 4000 rpm for 15 minutes, and the supernatant was filtered to obtain supernatant I.

[0054] B) Adjust the pH value of the supernatant I to 6.5, heat at 80°C for 15 minutes, cool to 35°C and centrifuge at 4000 rpm for 20 minutes, take the supernatant and filter to obtain Serum II;

[0055] C) Freeze the supernatant II at -20°C, take it out after freezing, and melt it in a water bath at 12°C, and ultrafilter th...

Embodiment 2

[0059] This example provides a spleen extract containing 5.0 mg of polypeptide, 7.0 mg of free amino acid, 1.6 mg of nucleic acid, 180 μg of total sugar and molecular weight less than 8000 Dalton per ml. Its preparation steps are as follows:

[0060] A) Take 10kg of fresh healthy pig spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 20L water for injection, homogenate, adjust the pH value to 4.2, freeze at -18°C, take out in a water bath at 10°C after freezing Thaw, repeat freezing and thawing once, centrifuge the homogenate after the last thawing, the centrifugation condition is 3000 rpm, and the centrifugation time is 30 minutes, take the supernatant and filter to obtain supernatant I;

[0061] B) After adjusting the pH value of the supernatant I filtrate to 7.0, centrifuge, the centrifugation condition is 4000 rpm, and the centrifugation time is 30 minutes, take the supernatant and filter to obtain the supernatant II;

[0062] ...

Embodiment 3

[0066] This example provides a spleen extract containing 7.0 mg of polypeptide, 8.0 mg of free amino acid, 3.0 mg of nucleic acid, 360 μg of total sugar and molecular weight less than 5000 Dalton per ml. Its preparation steps are as follows:

[0067] A) Take 10kg of fresh healthy sheep spleen, remove impurities such as fascia and adipose tissue, wash it with water for injection, add 15L water for injection, homogenate, adjust the pH value to 3.0, freeze at -15°C, take it out in a water bath at 28°C after freezing Thaw, repeat freezing and thawing 4 times, centrifuge the homogenate after the last thawing, the centrifugation condition is 3500 rpm, the centrifugation time is 25 minutes, take the supernatant and filter to obtain supernatant I;

[0068] B) Adjust the pH value of the supernatant I filtrate to 6.0, heat at 75°C for 15 minutes, cool to 25°C and centrifuge at 3500 rpm for 30 minutes, then filter the supernatant, Clear solution II;

[0069] C) Supernatant II was froze...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a spleen extract prepared by extracting and preparing the spleen tissue of mammals other than humans. The spleen extract is obtained through the steps of fat removal, homogenization, freeze-thaw crushing, precipitation, and ultrafiltration of the spleen tissue of mammals. , containing the following components: polypeptides, free amino acids, nucleic acids, total sugars, all of which have molecular weights less than 10,000 Daltons; Thrombocytopenia, multiple infection syndrome, etc.), respiratory and lung infections, habitual cold, chronic hepatitis B, mumps, recurrent aphthous ulcers and other diseases, in the preparation and treatment of leukopenia caused by radiotherapy and chemotherapy, leukemia, Application of drugs in aplastic anemia, lymphoma and other malignant tumors, improvement of malignant transformation of tumor patients, virus infection, improvement of postoperative or adjuvant drugs in severe patients who are weak.

Description

【Technical field】 [0001] The present invention relates to a spleen extract, its preparation method and its use, in particular to a spleen extract prepared by extracting and separating from the spleen tissue of mammals other than humans, its preparation method and its use, belonging to field of pharmaceutical preparations. 【Background technique】 [0002] As we all know, there are many various nutrients and active ingredients needed by the human body in mammals, and there are many reports on the extraction of active polypeptides from the heart, liver and muscle tissue of mammals. [0003] The spleen is the peripheral immune organ of mammals, and it is also one of the organs with the most vigorous metabolism in animals. Because it is rich in basic cell nutrients and cytokines, especially active ingredients that promote cell metabolism such as polypeptides, free amino acids, Nucleic acid and various sugars have received extensive attention from researchers. The research work o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/55A61K38/17A61K9/08A61K9/19A61K9/20A61K9/48A61P37/02A61P17/00A61P11/00A61P1/16A61P1/02A61P35/00
Inventor 郭智华
Owner 融致丰生制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com