Spleen polypeptide extract, its preparing process and use
A technology of extract and spleen, applied in the field of spleen polypeptide extract, to achieve the effect of improving malignant transformation, definite curative effect and low cost
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Embodiment 1
[0044] step 1):
[0045] A) Take 10kg of fresh healthy calf spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 10L water for injection, homogenate, adjust the pH value to 3.8, freeze at -20°C, take out after freezing and place in a water bath at 15°C Thaw in medium, repeat freeze-thaw 2 times.
[0046] B) Centrifuge the homogenate after the last melting, the centrifugation condition is 4000 rpm, the centrifugation time is 15 minutes, the supernatant is filtered through the pulp, and the filtrate is adjusted to pH 6.5, heated at 80°C for 15 minutes, cooled After reaching 35°C, centrifuge at 4000 r / min for 20 minutes, filter the pulp to obtain the supernatant;
[0047] C) Freeze at -20°C, take out after freezing and melt in a 12°C water bath, after the pulp is filtered, the supernatant is ultrafiltered once with an ultrafiltration membrane (U.S. millipore company, standard ultrafiltration system) with a molecular weight cut-off of 60...
Embodiment 2
[0051] step 1):
[0052] A) Take 10kg of fresh healthy pig spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 20L water for injection, homogenate, adjust the pH value to 4.2, freeze at -18°C, take out in a water bath at 10°C after freezing Thaw, repeat freeze-thaw once.
[0053] B) Centrifuge the homogenate after the last melting, the centrifugation condition is 3000 rpm, the centrifugation time is 30 minutes, the supernatant is filtered through the pulp, and the filtrate is adjusted to pH 7.0, heated at 70°C for 20 minutes, cooled After reaching 20°C, centrifuge at 4,000 rpm for 30 minutes, filter the pulp to obtain a clear liquid, and freeze at -18°C;
[0054] C) after freezing, take out and melt in a water bath at 25°C. After the pulp is filtered, the supernatant is first ultrafiltered once with an ultrafiltration membrane (U.S. millipore company, standard ultrafiltration system) with a molecular weight cut-off of 10,000, and th...
Embodiment 3
[0058] step 1):
[0059] A) Take 10kg of fresh healthy sheep spleen, remove impurities such as fascia and adipose tissue, wash it with water for injection, add 15L water for injection, homogenate, adjust the pH value to 3.0, freeze at -15°C, take it out in a water bath at 28°C after freezing Thaw, repeat freeze-thaw 4 times.
[0060] B) Centrifuge the homogenate after the last melting, the centrifugation condition is 3500 rpm, the centrifugation time is 25 minutes, the supernatant is filtered through the pulp, and the filtrate is adjusted to pH 6.0, heated at 75°C for 15 minutes, cooled After reaching 25°C, centrifuge at 3500 rpm for 30 minutes, filter the pulp to obtain a supernatant, and freeze at -15°C.
[0061] C) after freezing, take out and melt in a water bath at 15°C. After the pulp is filtered, the supernatant is first ultrafiltered once with an ultrafiltration membrane (U.S. millipore company, standard ultrafiltration system) with a molecular weight cut-off of 8000,...
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