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Use of shamrock extract in preparing anti-tumor medicament

An anti-tumor drug, the technology of clover, applied in the direction of anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., to achieve the effect of simple method and low cost

Inactive Publication Date: 2012-05-02
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of clover in the preparation of antitumor drugs

Method used

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  • Use of shamrock extract in preparing anti-tumor medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the preparation of clover extract

[0018] Get clover (Ningbo Dekang Biological Products Co., Ltd.) 200g, mix it with 95% ethanol (ethanol: water=95: 5, volume ratio) with 1: 3 (clover: 95% ethanol) in parts by weight ratio, in Reflux extraction at 60°C for 3 hours, filter with suction, collect the first reflux extract 1 and filter residue 1; mix all the obtained filter residue 1 with 95% ethanol, the amount of 95% ethanol is the same as the first time, at 60°C Under extraction for 3 hours, collect the second reflux extract 2 and filter residue 2, and combine the extract 2 with the extract 1; mix all the filter residue 2 obtained with 95% ethanol, the amount of 95% ethanol is the same as the first time, Reflux extraction at 60°C for 3 hours, suction filtration, and collect the third reflux extract 3; combine extract 1, extract 2 and extract 3, concentrate the obtained extract under reduced pressure, and vacuum dry (remove ethanol and water ) to constant w...

Embodiment 2

[0019] Embodiment 2, the application of clover extract in inhibiting tumor cell growth

[0020] The logarithmically grown tumor cells (human liver cancer cell BEL-7402, human colon cancer cell HCT-8, human lung cancer cell A-549) were digested with 0.25% trypsin, and then treated with 10% (volume percentage) fetal Suspend the cells in DMEM complete medium of bovine serum to make a cell suspension, and dilute each tumor cell to 1.0×10 5 cells / mL, and then inoculated into 96-well culture plate, 200uL per well, each tumor cell was inoculated into 6 wells (including 3 in the experimental group and 3 in the control group), and placed in an incubator with 5% CO2 at 37°C Incubate for 24 hours; then pour out the culture medium in each well.

[0021] The clover extract prepared in Example 1 was dissolved in DMSO, and then diluted with DMEM complete medium to obtain a solution of the clover extract, the final concentration of the clover extract was 50ug / mL, wherein the concentration of...

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Abstract

The present invention discloses a new usage of clover extract. The usage is the application of clover extract in preparing anti-tumor drugs. The extract is prepared according to the following method: taking clover as the raw material and extracting by water and / or organic solvent. The clover extract provided by the present invention has important significance to preparing anti-tumor drugs, especially the drugs for resisting liver cancer, lung cancer and colon cancer.

Description

technical field [0001] The invention relates to the application of clover extract in the preparation of antitumor drugs. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage, but once symptoms appear and go to the doctor, it is already in the middle and late stages. Therefore, the treatment of liver cancer is very difficult, and the average patient's survival time after onset is only 6 months, which is called "the king of cancer". According to the survey results, the top three new cancers in men are lung cancer, colon cancer, and nasopharyngeal cancer, and breast cancer, colon cancer, and lung cancer in women, which fully demonstrates the harm of lung cancer and colon cancer in the new cancer group sex. [0003] Fi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P35/00
Inventor 唐亚林谭莉戴玮刘岩向俊锋
Owner INST OF CHEM CHINESE ACAD OF SCI