Selenium-rich Enterococcus faecium cernelle three-layer microcapsule and preparation method and use thereof

Abstract

The invention provides a selenium-enriched lactic acid enterococcus faecalis three-layer microcapsule and a preparation method thereof. The microcapsule sequentially comprises a selenium-enriched lactic acid enterococcus faecalis, nutritive layer, an anti-acid anoxybiotic layer and a compression-resisting and breakage-proof layer from the inside to the outside. Wherein culture conditions of the selenium-enriched lactic acid enterococcus faecalis are 25-35 mg / l of selenium, 5-15 g / l of inoculum size, 28-32 h of fermentation time and 4.5-6.3 of pH when fermenting in an anaerobic jar; based on a double-layer embedding, the embedding method of the three-layer microcapsule is mainly mixing the three-layer microcapsule together with 2.0-2.5% of glutin aqueous solution and 0.1-0.3% of sodium alginate aqueous solution to make a suspension; an improved low-temperature spray drying method is adopted to form the compression-resisting and breakage-proof layer and to make the embedded selenium-enriched lactic acid enterococcus faecalis three-layer microcapsule have gastric acid-resisting, storage wearing, thermal stabilizing and compression-resisting and breakage-proof functions based on theoriginal biological function and organic selenium supplementing function. So the microcapsule is widely applied to producing feeds.

Description

technical field [0001] The invention relates to a selenium-enriched enterococcus faecalis three-layer microcapsule and its preparation method and application, which are used for the supplement of organic selenium in the breeding of livestock, poultry and wild animals, the prevention of digestive tract diseases, and the improvement of the balance of intestinal microecology. The field of microecological feed additives. Background technique [0002] The lactic acid bacteria biological fermentation products added in the feed mainly include bifidobacteria (Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium bifidum and Bifidobacterium adolescentis), lactic acid bacteria (Lactobacillus acidophilus and Lactobacillus casei ), Enterococci (Urinary Enterococcus and Faecalis Enterococcus), etc. Its mechanism of action is mainly reflected in 1) maintaining the normalization of the digestive tract flora and maintaining the dominant position of the beneficial bacteria flora; 2...

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