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Special solid complex enzyme (ALD, TIM and GDH) reagent for determining content of fructose diphosphate sodium (FDP)

A technology of sodium fructose diphosphate and compound enzyme, which can be used in the determination/inspection of microorganisms, lyase, isomerase, etc., and can solve the problem of no similar products.

Active Publication Date: 2010-04-07
张莉莉
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  • Application Information

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Problems solved by technology

After years of development, research and production of solid compound enzyme (ALD, TIM, GDH) reagents fo

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  • Special solid complex enzyme (ALD, TIM and GDH) reagent for determining content of fructose diphosphate sodium (FDP)
  • Special solid complex enzyme (ALD, TIM and GDH) reagent for determining content of fructose diphosphate sodium (FDP)
  • Special solid complex enzyme (ALD, TIM and GDH) reagent for determining content of fructose diphosphate sodium (FDP)

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Abstract

The invention discloses a new special solid complex enzyme (ALD, TIM and GDH) reagent for determining the content of fructose diphosphate sodium (FDP) and a preparation method and an application method thereof. The solid complex enzyme comprises aldolase (ALD), triosephosphate isomerase (TIM) and glycerol phosphate dehydrogenase (GDH). In the invention, the solid complex enzyme reagent containing the aldolase (ALD), the triosephosphate isomerase (TIM) and the glycerol phosphate dehydrogenase (GDH) is extracted from animal muscles as main raw materials under a certain condition. The solid complex enzyme can determine the content of the fructose diphosphate sodium under a certain condition, and has the characteristics of strong specificity (the solid complex enzyme reacts with only the fructose diphosphate sodium and does not react with glucose, fructose, glucose 6-phosphate or fructose 6-phosphate), good repeatability (RSD=1.45%, n=6), higher accuracy and simple and convenient operation.

Description

[0001] A special solid composite enzyme (ALD, TIM, GDH) reagent for the determination of sodium fructose diphosphate (FDP) content and its preparation and application method. technical field [0002] The invention relates to a special solid composite enzyme (ALD, TIM, GDH) reagent for the determination of fructose diphosphate sodium (FDP) content and a preparation method thereof. Background technique [0003] The existing (trial) standard determination methods of sodium fructose diphosphate in Chinese Pharmacopoeia include enzymatic method and diphenylamine method. Good specificity. Therefore, in the National Pharmacopoeia Commission's standards for sodium fructose diphosphate products, it is necessary to change the diphenylamine method in the determination method of sodium fructose diphosphate to an enzymatic method. However, the aldolase (ALD), triose phosphate isomerase (TIM) and glycerol phosphate dehydrogenase (GDH) used in the enzymatic method are currently not availa...

Claims

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Application Information

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IPC IPC(8): C12N9/90C12N9/88C12N9/04C12Q1/533C12Q1/527C12Q1/32
Inventor 不公告发明人
Owner 张莉莉
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