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Micro-RNA expression profiling and targeting in peripheral blood in lung cancer

A technology for peripheral blood and lung cancer, applied in biochemical equipment and methods, microbiological determination/testing, anti-tumor drugs, etc., can solve the variability of histological classification, the failure to solve the effectiveness of gene expression and biological correlation, etc. question

Inactive Publication Date: 2010-12-15
THE OHIO STATE UNIV RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, while reproducibility in lung cancer research has improved, variability remains in histologic classification using microarray analysis
Furthermore, genomic studies have not addressed the lack of availability of gene expression and biological relevance

Method used

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  • Micro-RNA expression profiling and targeting in peripheral blood in lung cancer
  • Micro-RNA expression profiling and targeting in peripheral blood in lung cancer
  • Micro-RNA expression profiling and targeting in peripheral blood in lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Peripheral whole blood microRNA expression correlated with previously reported primary tumor expression of specific microRNAs.

[0123] The inventors identified miRNAs that were downregulated in both lung tumor and peripheral blood samples from subjects with advanced lung cancer. See Table 1, which shows that miRNAs are altered in the peripheral blood of a group of subjects.

[0124] Table 1 shows decreased and increased miRNAs in lung cancer compared to normal levels in whole blood. Scores (d), fold changes and q-values ​​(%) are shown.

[0125]

[0126]

[0127]

Embodiment 2

[0129] Furthermore, the inventors confirmed the whole peripheral blood expression of a specific miRNA (miR-126) by RT-PCR in the case of non-small cell lung cancer (NSCLC) (n-4) compared to normal controls (n=3) mode (see figure 2 ).

Embodiment 3

[0131] In situ hybridization can be used to identify miRNA localization in mammalian tissues.

[0132] miRNA expression profiling of lung tissue distinguishes lung cancer from normal lung tissue. In situ hybridization studies were used to localize potential miRNAs in human lung cancer tissue samples. In one non-limiting example, miR-155 expression is increased in several solid tumors and hematologic malignancies. In lung cancer, increased miR-155 expression correlates with poor survival.

[0133] However, the localization and regulation of miR-155 expression in lung cancer remains unclear. As shown herein, the immature form of miR-155 has been identified in the nucleus of cancer cells of adenocarcinoma, but the mature form is not easily identified. (see Figure 3A , 3B ).

[0134] While not wishing to be bound by theory, the inventors herein believe that this shows that impaired processing of miR-155 is a possible mechanism of regulation in NSCLC. In bronchoalveolar cel...

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Abstract

A method for the diagnosis, prognosis and treatment of lung cancer by detecting at least one microRNA in peripheral blood is disclosed.

Description

[0001] Inventors: Serge P. Nana-Sinkam, Clay B. Marsh, Melissa G. Hunter, Gregory A. Otterson [0002] Cross-references to relevant applications and statements regarding federally sponsored research [0003] This application claims the benefit of US Provisional Application 61 / 004,863, filed November 30, 2007, the disclosure of which is incorporated herein by reference. This invention was made without Government sponsorship, and the Government has no rights in this invention. [0004] Technical field and industrial applicability of the invention [0005] The present invention relates to certain methods of diagnosing, prognosing and treating lung cancer by detecting at least one microRNA (miR) in peripheral blood. Background of the invention [0006] Lung cancer is the leading cause of cancer death in US men and women, with a dismal 5-year survival rate of less than 15%. Over the past few years, epidemiological statistics have shown that most lung cancers are diagnosed in for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12P19/34
CPCC12Q2600/178C12Q2600/112C12Q1/6886C12Q2600/158C12Q2600/106C12Q2600/136A61P35/00C12Q1/6809C12Q2525/207
Inventor S·P·纳那-辛克哈姆C·B·马什M·G·皮珀G·A·奥特森
Owner THE OHIO STATE UNIV RES FOUND
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