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New skin sampling kit which stores nucleic acids in stable status, genetic test methods by using the kit and their practical application

A kit and gene technology, applied in the field of skin gene card, can solve the problems of limited accuracy and effect, failure to monitor physiology, function, biochemistry, etc.

Inactive Publication Date: 2011-03-23
GOODGENE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0026] However, all the aforementioned tests only monitor structural changes of skin lesions under the microscope, but fail to monitor physiological, functional, biochemical, and their molecular and genetic alterations
Therefore, they have limitations in accuracy and effectiveness

Method used

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  • New skin sampling kit which stores nucleic acids in stable status, genetic test methods by using the kit and their practical application
  • New skin sampling kit which stores nucleic acids in stable status, genetic test methods by using the kit and their practical application
  • New skin sampling kit which stores nucleic acids in stable status, genetic test methods by using the kit and their practical application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0109] Preparation and performance verification of skin gene card obtained from skin samples

[0110] In this step, the skin gene card was prepared and its preparation method was established. Furthermore, the method of using the card to obtain the best skin sample and the conditions for storing and transporting the sample are established. Furthermore, conditions for isolating DNA and RNA from skin samples and conditions for cDNA synthesis were established. In addition, the quality and quantity of the separated DNA and RNA were verified.

[0111] The details are as follows.

Embodiment 1

[0112] Example 1. Preparation of skin gene card

[0113] The skin gene card of the present invention includes a belt part and a substrate (card) part. The belt is used to obtain tissues of the human body by being attached to and removed from the human body, and the card part is used to protect, store and transport the belt. The tape can be any type of tape. In one embodiment, a bandage that is harmless to the human body and allows medical use, especially a soft, low-viscosity paper bandage, can be used. In particular, 3M's low-tack paper bandages 1500, 1522 or 9874 can be used. The substrate (card) part can be a synthetic resin, paper card or film, glass slide, plastic product, or fiber product treated with diethyl pyrocarbonate (DEPC). The substrate part can form a stable compound with DNA and RNA In order to prevent DNA and RNA from being degraded by deoxyribonuclease and ribonuclease, respectively, and store them stably at room temperature. Further, the substrate may be pa...

Embodiment 2

[0116] Example 2. Method for obtaining skin samples using skin gene cards

[0117] Apply peeling gel on and around the sampling point. The cutin is removed by rubbing with hands, and the peeling gel is completely removed with ethanol. The skin gene card of the present invention is attached to the sampling point, and the cover of the belt part is removed. Soon, the card was removed. In one embodiment, acnepris (Biolee) can be used for peeling gels. However, any gel used for skin cleansing can be used. The duration of the sticking to the skin can be from 1 minute to 12 hours, usually 30 minutes. The card of the present invention can be attached to any part of the skin. In order to perform cosmetic treatments for those without skin diseases, samples can be taken from the forehead, nose, chin, around the eyes or cheeks normally. In order to diagnose those suspected of having skin diseases, samples can be taken directly on the damaged part. In this case, it is important to also...

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Abstract

The present invention relates to a new skin gene card for genetic test, a method for acquiring DNA and RNA and performing various genetic tests using the same, and practical applications thereof. More specifically, the inventors of the present invention have developed a skin gene card capable of acquiring samples from human skin, hair or mucosa simply, safely and quickly and enabling stable long-term storage and transport of DNA and RNA included in the acquired sample at room temperature. Various genetic tests may performed using the acquired DNA and RNA, including polymerase chain reaction (PCR), reverse transcription (RT)-PCR, real-time PCR, sequencing, hybridization, DNA chip analysis, single-nucleotide polymorphism (SNP) assay, gene mutation assay, promoter methylation assay, gene expression assay, etc. The genetic skin test result may be utilized for disease prognosis, nutrigenomic test, pharmacogenomic test, forensic test such as personal identification, diagnosis of genetic diseases, diagnosis of skin diseases, or the like. In addition, through an objective evaluation of the skin or hair condition, a personalized cosmetic and skin care system may be established for practical application in beauty care, cosmetology, dermatology, and clinical practice.

Description

Technical field [0001] The invention relates to a new skin gene card used for genetic testing, methods for obtaining DNA and RNA using the gene card and conducting various genetic tests and practical applications. More specifically, the inventors of the present invention have developed a skin gene card that can simply, safely and quickly obtain samples from human skin, hair or mucous membranes and can stably store and transport DNA and RNA included in the resulting samples at room temperature for a long time. Use the obtained DNA and RNA to perform various genetic tests, including polymerase chain reaction (PCR), reverse transcription (RT)-PCR, real-time PCR, sequencing, hybridization, DNA chip analysis, single nucleotide polymorphisms (SNP) analysis, gene mutation analysis, promoter methylation analysis, gene expression analysis, etc. The genetic skin test result can be used for disease prognosis, nutrigenomic test, pharmacogenomic test, forensic examination, such as identific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCA61B10/02
Inventor 文宇哲李振荣朴根良申贞植
Owner GOODGENE
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