Method for producing pinellia ternate embryoid and tissue culture seedling

A technology of embryoid bodies and tissue culture seedlings, which is applied in the field of simple and efficient production of Pinellia pinellia embryoid bodies and tissue culture seedlings, and can solve the problems that the production data of Pinellia pinellia embryoid bodies and tissue culture seedlings have not been retrieved.

Inactive Publication Date: 2011-08-24
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

However, no data have been retrieved for the production of pinellia embryoid bodies and tissue cultured plantlets

Method used

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Embodiment

[0009] The applicant utilizes the characteristics that LFS possesses the biological activity of two types of hormones, CTK and Auxin. After repeated screening of the appropriate concentration and comparative research with conventional methods, it is found that: using MS+LFS 0.3mg·L -1 This single culture medium uses Pinellia bulbs as explants. After obtaining aseptic seedlings according to conventional culture, cut petioles (or other isolated organs such as leaves) as subculture propagation materials. This single medium is still used. The subculture of the medium can induce one or both ends of the cut petiole to rapidly form embryogenic callus and differentiate into embryoid bodies, with an induction rate of 80-100%; embryoid bodies can be used to prepare artificial seeds and can continue to germinate Grow into tissue culture seedlings. After one subculture, the tissue cultured seedlings (not counting ungerminated embryoid bodies and those whose height is less than or equal to...

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Abstract

The invention relates to a method for simply and efficiently producing pinellia ternate embryoid and tissue culture seedling. The method is characterized in that an MS (Murashige and Skoog) solid culture medium is used in a production process, lingfasu(LFS) with the concentration of 0.3mg/L (the concentration is effective in the range from 0.05 to 2.0) is added into the MS solid culture medium to be prepared into a 0.3mg/L MS+LFS single culture medium, pinellia ternate petiole slices (or blades or other separated organs) are taken as a successive propagation material, and all the related technical links such as 'callus induction', 'embryoid induction', 'mature seedling induction', 'multiplication and propagation', 'rooting' and the like can be completed in one step by using the single culture medium. The embryoid inductivity reaches 80-100%; one successive cycle comprises 30 days, multiplication times of the tissue culture seedling can reach 10-20 or higher. By applying the method, the production process of pinellia embryoid and tissue culture seedling is simplified, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to a simple and efficient method for producing pinellia pinellia embryoid bodies and tissue culture seedlings. Background technique [0002] Pinellia is a perennial herbaceous plant of Araceae. Pinellia ternata (Thunb) Briet recorded in Chinese Pharmacopoeia is an important traditional Chinese medicinal material and export material. Antiemesis, the effect of dispelling lump and dissipating stagnation, is used externally to treat carbuncle. Because the underground tubers of pinellia are used as medicine, with the continuous increase of market demand, excessive harvesting and random digging are very serious, making wild resources endangered. In order to solve the contradiction between resource protection and market demand, the research and application of Pinellia tissue culture and rapid propagation technology has been carried out rapidly in China in recent years. The results of comprehensive multi-channel retrieval show that at p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 许鸿章许鸿源解云英陈汝贤梁琼月
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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