Three-dimensional cell dynamic culture reactor

Abstract

The invention discloses a three-dimensional cell dynamic culture reactor. An internal shell of the reactor is arranged in the external shell of the reactor with a clearance in between; the lower part of the left side of the external shell of the reactor extends into an air inlet pipe in the lower internal part of the internal shell of the reactor; the upper end of the internal shell of the reactor is provided with a sealing upper cover with an exhaust one-way valve; the side wall of the internal shell of the reactor is provided with a liquid outlet pipe; the lower internal part of the internal shell of the reactor is provided with a supporting plate in which a vent hole is formed; the supporting plate is provided with a lower waterproof breathable film of which the upper part is provided with a cell culture bracket; the upper surface of the cell culture bracket is provided with a waterproof breathable film; the lower end of the liquid inlet pipe is inserted onto the upper surface of the lower water; the liquid inlet pipe is communicated with the outlet of a peristaltic pump; the inlet of the peristaltic pump is communicated with a cell suspension bottle, a cell culture solution bottle and a buffer solution bottle; and the liquid outlet pipe is communicated with a cell suspension bottle, a cell culture solution bottle and a waste liquid bottle. When the reactor is used, cell dynamic inoculation and cell dynamic culture are realized, equipment is reduced, and the cell culture time is reduced. The reactor can be used for culturing adherent cells.

Description

technical field [0001] The invention belongs to the technical field of cell culture equipment or devices, and in particular relates to culture equipment using flowing cell culture fluid in a carbon dioxide environment. Background technique [0002] Traditional cell culture methods, namely two-dimensional cell culture, cause cells to lose their tissue-related functions and cannot accurately reflect the growth of cells in vivo. Although animal models and in vivo experiments can reflect the real internal environment of cell growth, the cost is too high, the process is complicated, and they are restricted by ethics. In vivo environment, all cells are in a kind of three-dimensional space. Three-dimensional cell culture can reproduce cell-cell and cell-matrix interactions, and compared with two-dimensional cell culture, it can more truly reflect the internal environment of cell growth. Therefore, 3D cell culture can bridge the gap between 2D cell culture and animal experiments. ...

AI Technical Summary

Problems solved by technology

These bioreactors have a single function, some instruments are used for cell separation or collection, some are used for the preparation of cell suspension, some are used for mixing cells and materials, some are used for cell and tissue culture, The main disadvantages of these bioreactors are operational complexity, susceptibility to contamination

[0005] The invention patent with the patent number ZL 031106701.2 and the invention name "Cell Reactor", the patent application number is 200910229109.1, the invention patent with the invention name "Polysaccharide Composite Scaffold Perfusion Hepatocyte Reactor System for Evaluation of Drug Hepatotoxicity", patent application No. 200910242907.8, the invention title is "A Cell Reactor and Artificial Liver Support System Including the Reactor". None of these three cell reactors can provide the temperature, humidity and gas conditions required for cell growth. The cell reactor can only be used if it is placed in a carbon dioxide incubator, and the cell reactor and a cell reactor and the artificial liver support system including the reactor cannot realize dynamic cell seeding and dynamic culture

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