Stem cell expansion culture system and stem cell expansion culture method

Abstract

Provided are a stem cell expansion culture system and a stem cell expansion culture method. The stem cell expansion culture system includes: a cell culture box, which is used for accommodating a cell culture unit; a cell printing head, which is used for printing biological materials containing stem cells to the cell culture unit; a cell culture unit driving device, a cell culture unit The unit drive device is used at least for grabbing the cell culture unit from the cell culture cassette and / or for placing the cell culture unit in the cell culture cassette. The cell printing head and the cell culture unit driving device cooperate with each other to print the biological material containing stem cells on the cell culture unit, so that the stem cells can grow in a three-dimensional external environment, so that there is enough growth space, which can not only increase the number of stem cells. The differentiation potential is well preserved, and the frequency of cell passaging operations can be reduced, the risk of contamination can be reduced, and high-quality and high-efficiency stem cell culture can be achieved.

Description

technical field [0001] The present invention relates to the field of biomedical technology, and particularly relates to a stem cell expansion culture system and a stem cell expansion culture method. Background technique [0002] Stem cells have important applications in modern medicine and scientific research, but the efficient and high-quality expansion of stem cells has always been a difficult problem. [0003] In traditional cell culture or expansion, cells are planted in culture flasks, petri dishes, etc. When the cells proliferate to a certain density, the cells need to be digested, collected by centrifugation, and then dispersed into multiple culture flasks for continued cultivation. [0004] This method is simple and reliable, but it also has the following shortcomings: [0005] First, culture equipment such as culture flasks and culture dishes provide cells with a two-dimensional growth environment. Since the culture environment is very different from the three-dime...

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Problems solved by technology

[0006] Second, stem cells will experience contact inhibition after they proliferate to a certain density on a two-dimensional plane, and if the concentration is too high, stem cells will easily differentiate and lose their potential for differentiation, so that high-efficiency stem cell expansion cannot be achieved, and the output rate is not high.

[0007] Third, in large-scale culture, the cells need to change the medium every two days, and need to be subcultured in about four days. These daily maintenance operations require a lot of time and labor costs, and the labor costs are high.

[0008] Fourth, during the long-term cultivation process, it is necessary to repeatedly open the culture bottle for liquid replacement and passage operation, which greatly increases the risk of contamination

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