Stem cell expansion culture system and stem cell expansion culture method

Abstract

The present invention provides a stem cell expansion culture system and a stem cell expansion culture method. The stem cell expansion culture system comprises a cell culture box, a cell printing headand a cell culture unit driving device; the cell culture box is used for accommodating cell culture units; the cell printing head is used for printing a biological material containing stem cells to the cell culture units; and the cell culture unit driving device at least is used for grasping the cell culture units from the cell culture box and / or putting the cell culture units into the cell culture box. The cell printing head cooperates with the cell culture unit driving device to print the biological material containing the stem cells onto the cell culture units, so that the stem cells can grow in a three-dimensional external environment and thus have sufficient growth space, and the stem cell expansion culture system can better preserve various differentiation potentials of the stem cells, can also reduce frequency of cell passage operations, reduces risks of contamination, and achieves high-quality and high-efficiency stem cell culture.

Description

technical field [0001] The invention relates to the field of biomedical technology, and in particular to a stem cell expansion culture system and a stem cell expansion culture method. Background technique [0002] Stem cells have important applications in modern medicine and scientific research, but the efficient and high-quality expansion of stem cells has always been a problem. [0003] Traditional cell culture or expansion is to plant cells in culture flasks, culture dishes, etc. After the cells proliferate to a certain density, the cells need to be digested, collected by centrifugation, and then dispersed into multiple culture flasks for further culture. [0004] This method is simple and reliable, but it also has the following disadvantages: [0005] First, culture flasks, culture dishes and other culture equipment provide cells with a two-dimensional growth environment. Since the culture environment is very different from the three-dimensional environment in the body,...

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Problems solved by technology

[0006] Second, stem cells will experience contact inhibition after they proliferate to a certain density on a two-dimensional plane, and if the concentration is too high, stem cells will easily differentiate and lose their potential for differentiation, so that high-efficiency stem cell expansion cannot be achieved, and the output rate is not high.

[0007] Third, in large-scale culture, the cells need to change the medium every two days, and need to be subcultured in about four days. These daily maintenance operations require a lot of time and labor costs, and the labor costs are high.

[0008] Fourth, during the long-term cultivation process, it is necessary to repeatedly open the culture bottle for liquid replacement and passage operation, which greatly increases the risk of contamination

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