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Monkey severe generalized parkinsonism model and drug screening method thereof

A Parkinson's disease, systemic technology, applied in the field of monkey severe systemic Parkinson's disease model and its drug screening, can solve the problems of low success rate and large differences in modeling methods, and achieve high success rate and modeling operation Simple and low-cost effect

Active Publication Date: 2014-08-20
WEST CHINA HOSPITAL SICHUAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the establishment of a systemic monkey Parkinson's disease model by intramuscular injection at home and abroad; and the modeling methods vary greatly, and the success rate is not high

Method used

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  • Monkey severe generalized parkinsonism model and drug screening method thereof
  • Monkey severe generalized parkinsonism model and drug screening method thereof
  • Monkey severe generalized parkinsonism model and drug screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of the animal model of the present invention

[0026] 1. Materials and methods

[0027] 1. Selected experimental animals:

[0028] With reference to the inclusion criteria of experimental monkeys, 4 healthy adult macaques were purchased from Sichuan Pingan Experimental Animal Breeding Center. Age 5-7 years old, weight 5.1-6.5Kg, 2 males and 2 females, represented by M1, M2, M3, M4. After passing the inspection and quarantine, it will be placed in the National (Chengdu) Traditional Chinese Medicine Safety Assessment Center (GLP) Large Animal Breeding Center for breeding.

[0029] Inclusion criteria for experimental monkeys:

[0030] ①, age 4-7 years old;

[0031] ②. There are blood routine, biochemical and infectious diseases test reports, and the test results are normal without infectious diseases;

[0032] ③ No other experimental research has been accepted in the past;

[0033] ④ Weight 4-7Kg;

[0034] ⑤ Free movement of trunk, limbs and head and face;

[0035...

Embodiment 2

[0092] Example 2 Using classic drugs for the treatment of severe systemic Parkinson's disease to verify the animal model of systemic Parkinson's disease and initially establish a related drug screening system

[0093] a. Take the monkey severe systemic monkey Parkinson's disease model prepared in Example 1;

[0094] b. The currently recognized classic drugs for the treatment of severe systemic monkey Parkinson's disease (such as Medopa, Sining) and candidate drugs are respectively applied to the systemic Parkinson's disease model;

[0095] c. Compared with classic drugs for the treatment of severe systemic Parkinson's disease, observe the influence of candidate drugs on the symptoms of systemic Parkinson's disease. The symptoms of monkeys with severe systemic Parkinson's disease include: basic motor skills , Fine movement, tremor, bradykinesia program, stupor, movement, posture, startle response, weakened movement, balance, etc.; and quantitatively score them to evaluate potential dr...

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Abstract

The invention provides a preparation method of a monkey severe generalized parkinsonism model, comprising the step of applying 0.5mg / kg of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) to a monkey for once every day for four days. The invention also provides a method for screening drugs for treating severe generalized parkinsonism. In the invention, the modeling operation is simple, convenient and safe, the success rate is high, the cost is low and the typical generalized symptom of the monkey can be modeled.

Description

Technical field [0001] The invention relates to a monkey model of severe systemic Parkinson's disease and a drug screening method thereof. Background technique [0002] There are many kinds of experimental animal models of Parkinson's disease (PD), including models of surgical injury in mice and rats or models of damage by stereotactic injection of 6-hydroxydopa. The most valuable model animal should be a species with similar structure, function and complexity to the human nervous system, especially the basal ganglia of the midbrain. Primate PD animal models can reflect the basic characteristics of PD symptoms and are currently the closest animal models to human PD, providing a good foundation and platform for basic research on PD. [0003] Human Parkinson's disease is a chronic neurodegenerative disease that occurs in the central nervous system. The disease often develops from one limb to the opposite limb, and the symptoms gradually worsen. The current Rhesus monkey Parkinson’...

Claims

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Application Information

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IPC IPC(8): A61K49/00A01K67/027
Inventor 王伟李鹏王莉李宏霞刘文科
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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