Reagent for liver cancer monitoring, staging and prognosis risk assessment and method thereof

A technology for prognosis assessment and liver cancer, applied in the field of biomedicine, can solve the problems of missed diagnosis of liver cancer patients, false positives, false negatives, etc., and achieve the effect of improving the accuracy rate

Inactive Publication Date: 2012-07-11
陈翠英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low specificity of AFP detection, some false negatives and false positives may occur, and some patients with liver cancer may be missed.

Method used

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  • Reagent for liver cancer monitoring, staging and prognosis risk assessment and method thereof
  • Reagent for liver cancer monitoring, staging and prognosis risk assessment and method thereof
  • Reagent for liver cancer monitoring, staging and prognosis risk assessment and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Liver cancer G-Test method can detect the changes of N-oligosaccharide profile in liver cancer patients

[0033] We have carried out the G-Test method analysis on the sera of 471 patients collected above, and its operation is as follows: figure 1 There are roughly four steps shown: (1) collection and processing of sample serum; (2) labeling and separation of N-oligosaccharide chains; (3) analysis of N-oligosaccharide chain fingerprints or G-Test patterns; (4) G -Test each parameter control analysis. Such as figure 2 As shown, the G-Test spectrum of human serum probably shows nearly 10 N-oligosaccharide chain peaks, and different oligosaccharide chains show different mobility due to different molecular sizes, that is, the N-oligosaccharide chains shown on the G-Test spectrum Different peaks represent different oligosaccharide chains; the relative concentration of oligosaccharide chains is expressed in the measured peak height. We have performed quantitative calculat...

Embodiment 2

[0037] G-Test analysis method can predict the occurrence and development of fibrosis

[0038] As shown in Table 8, the G-Test analysis method of the present invention can be used to detect and track the development of fibrosis in patients with liver diseases. In patients with fibrosis, the content of galactose-deleted two-antennary N-oligosaccharide chains (NGA2F+NGA2FB) increased linearly with the progression of liver fibrosis (Fig. 8A-B), while three-antennary N-oligosaccharides - The oligosaccharide chain (NA3) has a linear decreasing trend ( Figure 8 C). Thus, the ratio [(NGA2F+NGA2FB) / NA3] of G-Fibrosis, which detects and tracks the occurrence and development of fibrosis, and the ratio of the sum of the two antennas lacking in galactose to the three antennas are generated. The ratio parameter of G-Fibrosis fibrosis is directly proportional to the development of fibrosis ( Figure 9 ). Liver fibrosis is the abnormal proliferation and deposition of fibrous connective t...

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Abstract

The invention discloses a composition for monitoring liver cancer or liver fibrosis risks, which is prepared from the following N-oligosaccharide chains: NA3F, NA2F, NA2FB, NA2G, NA2GF, NGA2F, NGA2FB and NA3. The composition can be used for preparing a reagent which is used for liver cancer early-diagnosis, liver cancer staging detection or prognosis assessment and can also be applied to liver fibrosis early-diagnosis or prognosis assessment. The invention also provides a method for monitoring liver cancer or liver fibrosis risks. According to the method, the sensitivity and stability of liver cancer or liver fibrosis diagnosis can be greatly increased. The method is greatly superior to the current AFP (alpha fetoprotein) detecting technique.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a reagent or composition for monitoring the risk of liver cancer or liver fibrosis Background technique [0002] Primary liver cancer (PLC), referred to as liver cancer, includes primary hepatocellular carcinoma, cholangiocarcinoma, mixed carcinoma, and hepatoblastoma, among which primary hepatocellular carcinoma is the most common. Primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the most common cancers in the world, and it is also one of the tumors with the fastest increasing incidence and the highest mortality rate in recent years. Every year, Chinese patients account for nearly half of the global liver cancer incidence, ranking second in cancer mortality in China. When people are infected with hepatitis B virus ((hepatitis virus B, HBV) or hepatitis C virus (HCV), it often leads to chronic liver fibrosis and gradually develops into liver cirrhosis. Statis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 陈翠英
Owner 陈翠英
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