Enzymatic extraction process of swainsonine

Abstract

The invention discloses an enzymatic extraction process of swainsonine, which comprises the following steps: (1) drying the straight Astragalus membranaceus, crushing it and passing it through a 40-80 mesh sieve; (2) using water with a pH of 4.5 as a solvent , adding cellulase and the Radix Astragali prepared in the step (1) to water; the amount of cellulase added is 1%-5% of the weight of Astragalus, and the weight-to-volume ratio of Astragalus to solvent is 1:10-50g / ml; (3) React at 50°C for 1-5h to obtain the extract of swainsonine. The enzymatic extraction process of swainsonine proposed by the invention has the advantages of high extraction rate, low energy consumption and short extraction time.

Description

technical field [0001] The invention relates to a plant extract, in particular to an enzymatic extraction process of swainsonine. Background technique [0002] Stem Straight Astragalus (Astragalus strictus) is a perennial plant of the leguminous Astragalus genus. It is distributed in all parts of Tibet, and a large number of livestock are poisoned to death every year in the pastoral areas where it grows densely. It is one of the most serious poisonous weeds in the pastoral areas of Tibet. The main toxic component in Astragalus radix is ​​swainsonine, which is a highly polar indolizidine alkaloid with excellent water solubility. After being absorbed by the animal intestine, it is decomposed in the acidic environment of lysosome Because its cationic structure is similar to that of α-mannose cation, it competes with α-mannosylase, which not only inhibits the enzyme activity of α-mannosylase, but also causes mannose in lysosomes to fail to metabolize and accumulate, thus Cause...

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Problems solved by technology

[0005] As described in the above-mentioned patents, the commonly used traditional methods for extracting effective plant components include water decoction, percolation, reflux extraction, continuous reflux extraction, etc., but these methods Both have the disadvantages of low extraction rate of active ingredients, high energy consumption, and long extraction time.

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