Extraction method of kumquat polysaccharides
An extraction method and kumquat technology, applied in the field of extraction of kumquat polysaccharides
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Embodiment 1
[0017] The kumquat dry powder is passed through a 40-mesh sieve, and added to the ethanol solution with a concentration of 70%. The mass ratio of the kumquat dry powder to the ethanol solution is 1:30. It is extracted by ultrasonic enhancement. The extraction temperature is 50°C, the extraction time is 90min, and the extraction is performed once. , the ultrasonic power is 200W, then centrifuge to obtain the supernatant and filter, evaporate and concentrate to 1 / 5 of the original volume, precipitate with ethanol, filter, and centrifuge the filtrate to obtain the crude extract. The measured polysaccharide extraction rate is 4.8%.
Embodiment 2
[0019] Kumquat dry powder is passed through a 40-mesh sieve and added to a 70% ethanol solution. The mass ratio of kumquat dry powder to ethanol solution is 1:30. Ultrasonic enhanced extraction is carried out at 50°C for 80 minutes for extraction twice. , Ultrasonic power is 180W, 220W respectively, then centrifuge to obtain the supernatant and filter, evaporate and concentrate to 1 / 5 of the original volume, precipitate with ethanol, filter, and centrifuge the filtrate to obtain the crude extract. The measured polysaccharide extraction rate is 7.2%.
Embodiment 3
[0021] Kumquat dry powder is passed through a 40-mesh sieve and added to a 70% ethanol solution. The mass ratio of kumquat dry powder to ethanol solution is 1:30. Ultrasonic enhanced extraction is carried out at a temperature of 50°C and an extraction time of 60 minutes for 3 extractions. , the ultrasonic power was 160W, 200W, 240W respectively, then centrifuged to obtain the supernatant and filtered, evaporated and concentrated to 1 / 5 of the original volume, precipitated with ethanol, filtered, and the filtrate was centrifuged to obtain the crude extract. The extraction rate of polysaccharide was measured 7.6%.
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