Methods for sequencing of partial ribose desoxyribonucleic acid (rDNA) of mitochondria 12s genes of brachionus rotifer and identifying brachionus rotifer
A technology of mitochondria and rotifers, applied in the biological field
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[0014] The biological materials used in this method for the identification of rotifers of the genus Brachionus are Brachionus rotifers, Brachionus calyxae, Brachionus acanthus, Brachionus square, Brachionus schizopoda, and Sickle rotifers. Brachionus rotifers, Brachionus tendigitata, Brachionus occipitalis, and Brachionus citriformum, pick a single individual of each rotifer, and culture them in monoclonal manner, among which Brachionus redus and Brachionus square , Brachionus citriformum, and Brachionus angularis were formulated by Gilbert (1963), and the other species used the original local water samples as culture medium after filtration. The temperature was basically consistent with the ambient temperature when they were collected. The food used was HB -4 culture medium, Scenedesmus obliquus in the exponential growth phase, when the number of single clone rotifers reaches a certain amount, filter the rotifers with a 25 μm pore size filter, and rinse with sterile distilled ...
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