Method for sequencing partial DNA of mitochondria ND5 gene of brachionus rotifera and authenticating brachionus rotifera
A DNA sequencing, mitochondrial technology, applied in the field of biology
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[0014] The biological materials used in this method for identifying rotifers of the genus Brachionus are Brachionus rubens, Brachionus quadridentatus, Brachionus falcatus, Brachionus diversicornis, tail process Brachionus caudatus, Brachionus caudatus, Brachionus caudatus, single individuals of each type of rotifer were picked and cultured in monoclonal manner, among them, Brachionus caudatus and Brachionus square used the formula of Gilbert (1963), and the others used the formula The original local water samples were filtered and used as culture medium. The temperature was basically the same as the ambient temperature at the time of collection. The food used was cultured with HB-4 culture medium and was in the exponential growth phase of Scenedesmus oblique. When the number of single rotifers reached When there is a certain amount, the rotifers are filtered out with a 25 μm pore size filter, rinsed with sterile distilled water, collected in Eppendof tubes, and stored in a -20°...
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