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A poorly differentiated thyroid cancer cell line PDTC-1 and applications thereof

A thyroid cancer and cell technology, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the lack of credible evidence-based medical evidence, the difficulty of large-sample randomized controlled studies, and the low incidence of anaplastic thyroid cancer And other issues

Inactive Publication Date: 2013-06-19
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

[0003] Due to the low incidence and high mortality rate of anaplastic thyroid cancer, it is difficult to conduct randomized controlled studies with large samples. Most of the literature reports are retrospective summaries and lack of reliable evidence-based medical evidence. Therefore, an animal xenograft tumor model was established. ideal for experimental research
There are no reports on cell lines and tumor models of poorly differentiated thyroid carcinoma

Method used

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  • A poorly differentiated thyroid cancer cell line PDTC-1 and applications thereof
  • A poorly differentiated thyroid cancer cell line PDTC-1 and applications thereof

Examples

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Embodiment 1

[0019] Example 1. Poorly differentiated thyroid cancer cell line and its preparation and application

[0020] 1. Preparation and verification

[0021] The present invention establishes the poorly differentiated thyroid cancer cell line PDTC-1 by the following method:

[0022] Cell separation and screening: The tumor specimen is an isolated specimen of poorly differentiated thyroid cancer. Fresh surgical specimens are taken, and the "fish-like" tumor tissue in the non-necrotic area is selected for cell separation and screening to obtain the target cell line, which is designated as PDTC-1 .

[0023] The cell line PDTC-1 was inoculated in PRIM 1640 medium containing 10% fetal bovine serum, and cultured in a 5% CO2 incubator at 37°C for 2 weeks to obtain the first-generation cultured cells; the first-generation cultured cells Cells were re-plated in PRIM 1640 medium with 10% fetal bovine serum at 37 °C in 5% CO 2 Cultured under incubator conditions for 2 weeks to obtain the sec...

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Abstract

The invention discloses a poorly differentiated thyroid cancer cell line PDTC-1 and applications thereof. The cell line is a poorly differentiated thyroid cancer cell line, and the accession number thereof is CGMCC No. 4939. The present invention establishes a poorly differentiated thyroid carcinoma cell line, successfully establishes a nude-mouse transplanted tumor model by using the cell line, provides a screenable cell line for the study of thyroid cancer cytology, and the cell line can be used for establishing important models for screening thyroid cancer-sensitive chemotherapy drugs. On the basis of the present invention, differences of differentiated, poorly differentiated and undifferentiated thyroid cancers can be studied from the genetic level and the protein level, having important meaning for elucidating the generating mechanism and finding therapeutic targets of the thyroid cancer. The cell line of the present invention provides a platform for the study of thyroid cancer evolution and biological characteristics of poorly differentiated cancers, and is conducive to the in-depth study of pathogenesis, metastasis and recurrence of thyroid cancer.

Description

technical field [0001] The invention relates to a poorly differentiated thyroid cancer cell line PDTC-1 and its application. Background technique [0002] Anaplastic thyroid cancer is a tumor with a high mortality rate. Although it accounts for 1%-5% of thyroid malignancies, the deaths from anaplastic thyroid cancer account for more than half of the deaths from thyroid cancer. With comprehensive treatment, the median survival period is only 2.9-10 months, the 1-year survival rate is 9.7%-37.8%, and the 3-year survival rate is 11.7%-31.2%. Poorly differentiated thyroid cancer is a special type of anaplastic thyroid cancer, which is a stage of evolution from well-differentiated thyroid cancer to undifferentiated thyroid cancer, so it is of great value for studying the biological characteristics of thyroid cancer. [0003] Due to the low incidence and high mortality rate of anaplastic thyroid cancer, it is difficult to conduct randomized controlled studies with large samples. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A61K49/00C12R1/91
Inventor 马洁安常明韩志楷王铮赵平
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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