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Method for cultivating pure aristichthys nobilis red.var groups

A breeding method and technology of golden bighead carp, which is applied in the field of breeding pure line populations of bighead carp, can solve the problems of high cost of induction method, complicated process, low hatching rate and survival rate, etc., and achieve the benefits of convenient batch processing and good quality , Improve hatching rate and survival rate

Active Publication Date: 2015-04-08
HUNAN XIANGYUN BIOLOGICAL
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Problems solved by technology

But so far, there are few reports on artificially inducing bighead carp to perform gynogenesis, because the operation of gynogenesis is easy to damage fish eggs, and there are many problems such as inappropriate sperm inactivation and improper treatment of diploid haploid eggs. For these reasons, the hatching rate and survival rate of artificially induced gynogenetic fish are not high. Related reports on breeding pure line populations of bighead carp by induced fish gynogenesis

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  • Method for cultivating pure aristichthys nobilis red.var groups
  • Method for cultivating pure aristichthys nobilis red.var groups
  • Method for cultivating pure aristichthys nobilis red.var groups

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Embodiment

[0044] A method for cultivating the Xiangyun golden carp pure line population of the present invention, specifically comprising the following steps:

[0045] 1. Sperm inactivation: Squeeze out the allotetraploid crucian carp that reached sexual maturity (the allotetraploid crucian carp was purchased from Hunan Xiangyun Biotechnology Co., Ltd., and its cultivation method can be found in Chinese Patent Document No. CN1263704A). In the petri dish, dilute the semen of allotetraploid crucian carp with Hank's solution, the volume ratio of Hank's solution to semen is 3:1, the diluted semen is distributed in a thin layer in the petri dish, put the petri dish containing semen Centrifuge on a shaker with ice packs at a speed of 45r / min, and irradiate under two UV lamps with a power of 15W. The distance between the UV lamp and the semen is 11cm, and the irradiation time is 30min. The tail swing of the sperm weakened and about 85% of the sperm still had good motility. At this time, the in...

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Abstract

The invention discloses a method for cultivating pure aristichthys nobilis red.var groups. The method comprises activating roe of aristichthys nobilis red.var with diploid sperms of allotetraploid crucian carps, performing cold shock treatment after activation, and controlling the temperature through flowing water to obtain gynogenesis aristichthys nobilis red.var; feeding feeds containing male hormone after incubation for several days to obtain sex reversal male gynogenesis aristichthys nobilis red.var; and performing selfing with gynogenesis aristichthys nobilis red.var serving as the female parent and with male gynogenesis gynogenesis aristichthys nobilis red.var serving as the male parent to obtain gynogenesis pure aristichthys nobilis red.var groups. Further, the gynogenesis aristichthys nobilis red.var can serve as the female parent, a common male aristichthys nobilis red.var serves as the male parent, and the female parent and the male parent are hybridized to obtain gynogenesis pure aristichthys nobilis red.var groups. According to the method, the roe hatchability and the survival rate can be improved greatly, and the pure aristichthys nobilis red.var groups stable in body color and excellent in performance can be cultivated.

Description

technical field [0001] The invention relates to a method for cultivating a fish population, in particular to a method for cultivating a pure line population of bighead carp. Background technique [0002] Hunan Xiangyun Biotechnology Co., Ltd. discovered individuals whose body color mutated into orange-red during the cultivation of common bighead carp. After long-term breeding, they obtained a new strain of bighead carp——Xiangyun golden carp (see figure 1 ) (Aristichthys nobilis red.var). Due to the advantages of fast growth, strong stress resistance, beautiful body shape, delicious taste, and orange-red fish body color, Xiangyun golden carp has been popular among fish farmers and consumers in recent years, and scale breeding has been carried out at present. And breeding, tens of thousands of acres of experimental breeding in Sichuan, Chongqing, Hunan and other provinces and cities, the effect is good. [0003] Since Xiangyun golden carp is a gene mutation strain, although ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 孙远东周工健刘少军杨福忠谭树华张小立李梦军
Owner HUNAN XIANGYUN BIOLOGICAL
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