Method for cultivating pure aristichthys nobilis red.var groups
A breeding method and technology of golden bighead carp, which is applied in the field of breeding pure line populations of bighead carp, can solve the problems of high cost of induction method, complicated process, low hatching rate and survival rate, etc., and achieve the benefits of convenient batch processing and good quality , Improve hatching rate and survival rate
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[0044] A method for cultivating the Xiangyun golden carp pure line population of the present invention, specifically comprising the following steps:
[0045] 1. Sperm inactivation: Squeeze out the allotetraploid crucian carp that reached sexual maturity (the allotetraploid crucian carp was purchased from Hunan Xiangyun Biotechnology Co., Ltd., and its cultivation method can be found in Chinese Patent Document No. CN1263704A). In the petri dish, dilute the semen of allotetraploid crucian carp with Hank's solution, the volume ratio of Hank's solution to semen is 3:1, the diluted semen is distributed in a thin layer in the petri dish, put the petri dish containing semen Centrifuge on a shaker with ice packs at a speed of 45r / min, and irradiate under two UV lamps with a power of 15W. The distance between the UV lamp and the semen is 11cm, and the irradiation time is 30min. The tail swing of the sperm weakened and about 85% of the sperm still had good motility. At this time, the in...
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