Method for rapidly identifying variety and quantity of species of moderately severe soda salinized grassland soil seed bank

A soil seed bank and salinization technology, which is applied in the preparation of test samples, germination equipment, sampling devices, etc., can solve the problems of time-consuming and difficult separation, and achieve the effect of soft texture

Inactive Publication Date: 2013-09-04
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of long time-consuming and difficult separation in the traditional method of identifying soil

Method used

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Experimental program
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specific Embodiment approach 1

[0016] Specific embodiment one: a kind of rapid identification method of the medium and severe soda salinization grassland soil seed bank species and the quantity method of this embodiment are realized according to the following steps:

[0017] 1. Take the depth from the soil surface to 5cm to 10cm in the middle and severe saline-alkali grassland, and the area is 10cm 2 ~50cm 2 The soil is a group, and every 3 to 8 groups are mixed to obtain a sample of the soil seed bank;

[0018] 2. Crush the large soil clods in the soil seed bank sample obtained in step 1, pick out the sundries other than seeds, put them in the room to air dry naturally, and then pass through a sieve with a diameter of 0.15mm, discard and sieve Soil, keep unsieved saline-alkali soil;

[0019] 3. Put the unscreened saline-alkali soil obtained in step 2 into a glass container, add water until the water surface is 0.5cm to 1cm higher than the soil surface, stir at a stirring speed of 60 / min to 120r / min for 3...

specific Embodiment approach 2

[0031]Embodiment 2: This embodiment is different from Embodiment 1 in that: in step 1, the soil is taken from the soil surface to a depth of 8 cm in the medium-severe salinization land. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0032] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: In step 3, water is added until the water surface is 0.5 cm to 0.8 cm higher than the soil surface. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a method for rapidly identifying the variety and the quantity of species of a moderately severe soda salinized grassland soil seed bank. The invention relates to a rapid identification method of the salinized grassland soil seed bank. The method disclosed by the invention aims to solve the problems that the consumed time is long and the separation is difficult in a conventional method for identifying the soil seed bank. The method disclosed by the invention comprises the following steps of: sieving the taken soil, adding acid into a soil sample water solution, agitating, filtering and standing to obtain seeds in the soil; washing by utilizing water and drying; and finally, recording and identifying the variety and the quantity of the species. Compared with a conventional direct manual picking method, the method is simple and feasible; the time is shortened by about 15 times and the efficiency is improved; the separation accuracy is guaranteed; the acid is added into the soil solution to neutralize alkaline salts in the soil; meanwhile, chemical properties and physical properties of the soil are changed and the problems that the salt content of soft soda saline-alkali soil is high, the pH (Potential of Hydrogen) is high, the physical properties are worsened and the like are solved; and the method disclosed by the invention can be used for rapidly identifying the moderately severe soda salinized grassland soil seed bank.

Description

technical field [0001] The invention relates to a rapid identification method for a salinized grassland soil seed bank. Background technique [0002] The area of ​​soda saline-alkali land in Songnen Plain is 2.333 million hectares, accounting for 15.2% of the total land area, and it is one of the three largest distribution areas of soda saline-alkali land in the world. The salt composition of saline-alkali soil is medium soda (NaHCO 3 with Na 2 CO 3 ) accounted for more than 90%, strong alkalinity, pH above 8.5, and moderate to severe saline-alkali land (pH9-10.5) accounted for the majority. The soil is highly dispersed, swells when wet, compacts when dry, and has poor permeability. In the 0-20cm soil surface layer, clay (particle size <0.002mm) and silt (0.002-0.02mm) are 57-70%, and the content of water-stable large aggregates in the soil is 0%. This type of soil mainly grows Suaeda corniculata, Puccinellia tenuiflora, Kochia sieversiana and other vegetation. The ...

Claims

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Application Information

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IPC IPC(8): G01N1/34A01C1/02G01N1/04
Inventor 马红媛吕丙盛梁正伟
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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